A5080854195- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- Bacteriophages and microbial interactions
- Network Security and Intrusion Detection
- Cancer Genomics and Diagnostics
- Peer-to-Peer Network Technologies
- RFID technology advancements
- Bacterial Genetics and Biotechnology
- Genomics and Phylogenetic Studies
- Genetics, Bioinformatics, and Biomedical Research
- Full-Duplex Wireless Communications
- Genomic variations and chromosomal abnormalities
- Cell Image Analysis Techniques
- Microbial Metabolic Engineering and Bioproduction
- Advanced Fluorescence Microscopy Techniques
- Energy Harvesting in Wireless Networks
- Image Processing Techniques and Applications
- Microbial Community Ecology and Physiology
- Pancreatic and Hepatic Oncology Research
- Lung Cancer Treatments and Mutations
- Evolutionary Game Theory and Cooperation
- Internet Traffic Analysis and Secure E-voting
- Fungal and yeast genetics research
Uppsala University
2022-2024
Science for Life Laboratory
2023-2024
Weizmann Institute of Science
2016-2021
Tel Aviv University
2012-2013
In experimental evolution, scientists evolve organisms in the lab, typically by challenging them to new environmental conditions. How best a desired trait? Should challenge be applied abruptly, gradually, periodically, sporadically? one apply chemical mutagenesis, and do strains with high innate mutation rate faster? What are ideal population sizes of evolving populations? There endless strategies, beyond those that can exposed individual labs. We therefore arranged community challenge,...
RNA splicing is a key process in eukaryotic gene expression, which an intron spliced out of pre-mRNA molecule to eventually produce mature mRNA. Most intron-containing genes are constitutively spliced, hence efficient crucial for regulation expression. Here we use large synthetic oligo library ~20,000 variants explore how different intronic sequence features affect efficiency and mRNA expression levels S. cerevisiae. Introns defined by three functional sites, the 5' donor site, branch 3'...
The localization of mRNAs encoding secreted/membrane proteins (mSMPs) to the endoplasmic reticulum (ER) likely facilitates co-translational translocation secreted proteins. However, studies have shown that mSMP recruitment ER in eukaryotes can occur a manner is independent ribosome, translational control, and signal recognition particle, although mechanism remains largely unknown. Here, we identify cis-acting RNA sequence motif enhances appears increase mRNA stability, both synthesis...
Splicing expands, reshapes, and regulates the transcriptome of eukaryotic organisms. Despite its importance, key questions remain unanswered, including following: Can splicing evolve when organisms adapt to new challenges? How does evolution optimize inefficiency introns' machinery? To explore these questions, we evolved yeast cells that were engineered contain an inefficiently spliced intron inside a gene whose protein product was under selection for increased expression level. We...
Abstract The DNA of bacterial cells is organized in a highly dynamic chromosome structure. To guarantee its propagation, the must replicate, segregate, and accommodate other biological processes like gene expression. Therefore, to understand causal relationship between organization function, it essential follow dynamics. Live-cell imaging fluorescent labels allows tracking specific genomic locations, but current imaging-based approaches study over cell cycle are limited their throughput....
Abstract The intracellular position of genes may impact their expression, but it has not been possible to accurately measure the 3D chromosomal loci. In 2D, loci can be tracked using arrays DNA-binding sites for transcription factors (TFs) fused with fluorescent proteins. However, same 2D data result from different trajectories. Here, we have developed a deep learning method super-resolved astigmatism-based localization in live E. coli cells which enables precision better than 61 nm at...
Abstract Optical pooled screening is an important tool to study dynamic phenotypes for libraries of genetically engineered cells. However, the desired engineering often requires that barcodes used in situ genotyping are expressed from chromosome. This has not been possible bacteria. Here we describe a method with genomic Escherichia. coli . The applied measure intracellular maturation time 81 red fluorescent proteins.
The computational modeling of chromatin structure is highly complex and challenging due to the hierarchical organization chromatin, which reflects its diverse biophysical principles, as well inherent dynamism, underlies complexity. variety methods for modeling, are based on different approaches, assumptions scales suggests that there a necessity comprehensive benchmark. This inspired us conduct project at NIH-funded 4D Nucleome Hackathon March 18-21, 2024 University Washington in Seattle,...
The availability of end-hosts and their assigned routable IP addresses has impact on the ability to fight spammers attackers, peer-to-peer application performance. Previous works study hosts mostly by using either active pinging or studying access a mail service, both approaches suffer from inherent inaccuracies. We take different approach measuring periodically reported uniquely identified group running DIMES agent. This fresh provides chance measure true dynamics addresses. Using two month...
Abstract RNA splicing is a key process in eukaryotic gene expression. Most Intron-containing genes are constitutively spliced, hence efficient of an intron crucial for Here we use large synthetic oligo library ~20,000 variants to explore how different intronic sequence features affect efficiency and mRNA expression levels S. cerevisiae . Using combinatorial design introns demonstrate non-consensus splice site sequences each the three sites. We then show that machinery tends select...