A5020425919- Estrogen and related hormone effects
- Liver Disease Diagnosis and Treatment
- Menopause: Health Impacts and Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Nuclear Receptors and Signaling
- Hypothalamic control of reproductive hormones
- Genomics, phytochemicals, and oxidative stress
- NF-κB Signaling Pathways
- Birth, Development, and Health
- Cholesterol and Lipid Metabolism
- Phytochemicals and Antioxidant Activities
- Adipose Tissue and Metabolism
- SARS-CoV-2 and COVID-19 Research
- Regulation of Appetite and Obesity
- Wound Healing and Treatments
- Lysosomal Storage Disorders Research
- Diet and metabolism studies
- Effects and risks of endocrine disrupting chemicals
- Reproductive System and Pregnancy
- Cytokine Signaling Pathways and Interactions
- SARS-CoV-2 detection and testing
- Skin Protection and Aging
- Advanced biosensing and bioanalysis techniques
- Digestive system and related health
- Phenothiazines and Benzothiazines Synthesis and Activities
University of Milan
2014-2024
Sapienza University of Rome
2009
Istituto di Chimica Biomolecolare
2009
University of Padua
2008
Institut Clinique de la Souris
2008
University of Parma
2008
Collège de France
2003
Estrogen is an immunoregulatory agent, in that hormone deprivation increases while 17beta-estradiol (E2) administration blocks the inflammatory response; however, underlying mechanism still unknown. The transcription factor p65/relA, a member of nuclear kappaB (NF-kappaB) family, plays major role inflammation and drives expression proinflammatory mediators. Here we report novel action E2 inflammation. We observe macrophages lipopolysaccharide-induced DNA binding transcriptional activity p65...
Abstract Fatty liver disease (FLD) caused by metabolic dysfunction is the leading cause of and prevalence rising, especially in women. Although during reproductive age women are protected against FLD, for still unknown understudied reasons some develop rapidly progressive at menopause. The patatin-like phospholipase domain-containing 3 ( PNPLA3 ) p.I148M variant accounts largest fraction inherited FLD variability. In present study, we show that there a specific multiplicative interaction...
Beyond the key role in reproductive and cognitive functions, estrogens have been shown to protect against neurodegeneration associated with acute chronic injuries of adult brain. Current hypotheses reconcile this activity a direct effect 17β-estradiol (E 2 ) on neurons. Here we demonstrate that brain macrophages are also involved E action Systemic administration hormone prevents, time- dose-dependent manner, activation microglia recruitment peripheral monocytes induced by intraventricular...
It has been previously demonstrated that 17beta-estradiol (E(2)) inhibits the response of microglia, resident brain macrophages, to acute injuries in specific regions. We here show effect E(2) inflammation is widespread and hormone reduces expression inflammatory mediators, such as monocyte chemoattractant protein-1, macrophage protein-2, TNF-alpha, induced by lipopolysaccharide, demonstrating microglia are a direct target estrogen action brain. Using APP23 mice, an animal model Alzheimer's...
Sex impacts on liver physiology with severe consequences for energy metabolism and response to xenobiotic, hepatic, extra-hepatic diseases. The comprehension of the biology subtending sex-related hepatic differences is therefore very relevant in medical, pharmacological, dietary perspective. extensive application metabolomics paired transcriptomics here shows that, case short-term fasting, decision maintain lipid synthesis using amino acids (aa) as a source fuel key discriminant male female...
Lipoprotein synthesis is controlled by estrogens, but the exact mechanisms underpinning this regulation and role of hepatic estrogen receptor α (ERα) in cholesterol physiology are unclear. Utilizing a mouse model involving selective ablation ERα liver, we demonstrate that couples lipid metabolism to reproductive cycle. We show regulates transport proteins, enzymes for lipoprotein remodeling, receptors uptake. Additionally, indispensable during proestrus generation high-density lipoproteins...
Although several lines of evidence have indicated that menopause is associated with increased susceptibility to neurological disorders, the mechanisms involved in this phenomenon remain be elucidated. Because neuroinflammation a common feature number brain diseases, we hypothesized cessation ovarian functions and consequent decrease estrogen receptor (ER)-mediated antiinflammatory activity may represent trigger for postmenopausal dysfunctions. The aim present study was investigate effects...
Among obesity-associated metabolic diseases, non-alcoholic fatty liver disease (NAFLD) represents an increasing public health issue due to its emerging association with atherogenic dyslipidemia and cardiovascular diseases (CVDs). The lower prevalence of NAFLD in pre-menopausal women compared men or post-menopausal led us hypothesize that the female-inherent ability counteract this pathology might strongly rely on estrogen signaling. In female mammals, receptor alpha (ERα) is highly expressed...
The loss of ovarian functions defining menopause leads to profound metabolic changes and heightens the risk developing dysfunction-associated steatotic liver disease (MASLD). Although estrogens primarily act on female through estrogen receptor alpha (ERα), specific contribution impaired ERα signaling in triggering MASLD after remains unclear.
In the search for xenoestrogens within food additives, we have analyzed Joint FAO-WHO expert committee database, containing 1500 compounds, using an integrated in silico and vitro approach. This analysis identified 31 potential estrogen receptor α ligands that were reduced to 13 upon applying a stringent filter based on ligand volume binding mode. Among xenoestrogens, four already known exhibit estrogenic activity, other nine assayed vitro, determining affinity biological effects. Propyl...
// Valeria Benedusi 1 , Elisa Martini 2 Marinos Kallikourdis 2, 3 Alessandro Villa Clara Meda Adriana Maggi Center of Excellence on Neurodegenerative Diseases and Department Pharmacological Biomolecular Sciences, University Milan, 20133, Italy Adaptive Immunity Laboratory, Humanitas Clinical Research Center, 20089 Rozzano, Medical Biotechnologies Translational Medicine, Correspondence to: Maggi, e-mail: adriana.maggi@unimi.it Keywords: female mammals, ovariectomy, life span, inflammation,...
Abstract In female mammals, the cessation of ovarian functions is associated with significant metabolic alterations, weight gain, and increased susceptibility to a number pathologies ageing. The molecular mechanisms triggering these systemic events are unknown because most tissues responsive lowered circulating sex steroids. As it has been demonstrated that isoform alpha estrogen receptor (ERα) may be activated by both estrogens amino acids, we test effects diet enriched in specific acids...
All the different coronavirus SARS-CoV-2 variants isolated so far share same mechanism of infection mediated by interaction their spike (S) glycoprotein with specific residues on cellular receptor: angiotensin converting enzyme 2 (ACE2). Therefore, steric hindrance this receptor created a bulk macromolecule may represent an effective strategy for prevention viral spreading and onset severe forms Corona Virus disease 19 (COVID-19). Here, we applied systematic evolution ligands exponential...
Abstract We have recently identified nip‐2 as a gene target for 17β‐oestradiol activity in the neuroblastoma SK‐ER 3 cells expressing oestrogen receptor (ER) α. Here we show treatment of and rat embryo neurones culture blocks increase mRNA induced by apoptotic stimuli prevents cell death indicated counting, 3,(4,5‐dimethylthiazol‐2‐yl)2,5‐diphenil‐tetrazoliumbromide DNA fragmentation assays. Neither these effects are observed presence specific ER antagonist ICI 182,780, absent not ER....
Estrogens play an important role in the regulation of energy homeostasis female mammals and a reduced ovarian function, due to natural aging or surgery, is associated with body weight increase fat redistribution. This disruption may constitute trigger for several pathologies known be climacterium; however, so far, limited attention has been devoted ability estrogen replacement therapies (ERT) reinstate balanced metabolism characteristic cycling mammals. The purpose present study was compare...
Non-alcoholic fatty liver disease (NAFLD) represents a public health issue, due to its prevalence and association with other cardiometabolic diseases. Growing evidence suggests that NAFLD alters the production of hepatokines, which, in turn, influence several metabolic processes. Despite accumulating on major role estrogen signaling sexually dimorphic nature NAFLD, dependency hepatokine expression sex estrogens has been poorly investigated. Through vitro vivo analysis, we determined extent...
Estrogen is known to affect vascular function and diabetes development, but the relative contribution of estrogen receptor (ER) isoforms unclear. The aim this study was determine how individual ER modulate inflammatory enzymes in wall control streptozotocin (STZ)-injected rodents. Primary cultures rat aortic smooth muscle cells (SMCs) were stimulated with agents presence or absence increasing concentrations ERα ERβ-selective agonists...
We previously demonstrated that Npy1rrfb mice, which carry the conditional inactivation of Npy1r gene in forebrain principal neurons, display a sexually dimorphic phenotype, with male mice showing metabolic, hormonal and behavioral effects females being only marginally affected. Moreover, exposure to high-fat diet (HFD) increased body weight growth, adipose tissue, blood glucose levels caloric intake compared Npy1r2lox controls. used knockout control examine whether disruption affects...
Microglia are heterogenous cells characterized by distinct populations each contributing to specific biological processes in the nervous system, including neuroprotection. To elucidate impact of sex-specific microglia heterogenicity susceptibility neuronal stress, we video-recorded with time-lapse microscopy changes shape and motility occurring primary derived from mice both sexes response pro-inflammatory or neurotoxic stimulations. With this morpho-functional analysis, documented...
New 17β-estradiol (E2) derivatives 1−11 were synthesized from an estrone derivative by addition of organometallic reagents prepared protected α,ω-alkynols and further elaboration the products. The estrogenic activity these novel compounds was determined using in vitro binding competition assay transactivation analysis. Among E2 synthesized, compound 2 showed highest potency therefore tested for its ability to modulate cutaneous wound healing vivo. Compound 2's accelerate ovariectomized mice...