A5089100479- Lipoproteins and Cardiovascular Health
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Cholesterol and Lipid Metabolism
- Cancer, Lipids, and Metabolism
- Peroxisome Proliferator-Activated Receptors
- Drug Transport and Resistance Mechanisms
- Sphingolipid Metabolism and Signaling
- Lipid metabolism and disorders
- Lysosomal Storage Disorders Research
- Adipokines, Inflammation, and Metabolic Diseases
- Cardiac Ischemia and Reperfusion
- Liver Disease Diagnosis and Treatment
- Metabolism and Genetic Disorders
- Calcium signaling and nucleotide metabolism
- Atherosclerosis and Cardiovascular Diseases
- Acute Myocardial Infarction Research
- Nitric Oxide and Endothelin Effects
- Phytoestrogen effects and research
- Apelin-related biomedical research
- Diabetes and associated disorders
- Pancreatic function and diabetes
- Mitochondrial Function and Pathology
- Analytical Methods in Pharmaceuticals
- Paraoxonase enzyme and polymorphisms
- Diet, Metabolism, and Disease
University of Milan
2014-2023
Centre for Human Drug Research
2017
Northwestern University
2014
Azienda Socio Sanitaria Territoriale Grande Ospedale Metropolitano Niguarda
2007-2013
University of Parma
2010
Istituti di Ricovero e Cura a Carattere Scientifico
2007
Centro Cardiologico Monzino
2007
National Institutes of Health
2007
University of Milano-Bicocca
2003
Paraoxonase-1 (PON1) is a high density lipoprotein (HDL)-associated serum enzyme that protects low lipoproteins from oxidative modifications. There relative lack of information on mechanisms implicated in PON1 release cells. The present study focused model derived stable transfection CHO cells, to avoid co-secretion apolipoprotein (apo) A-I and lipids, which could lead formation HDL-like complexes. Our results indicate that, the absence an appropriate acceptor, little released. designate HDL...
Lipoprotein synthesis is controlled by estrogens, but the exact mechanisms underpinning this regulation and role of hepatic estrogen receptor α (ERα) in cholesterol physiology are unclear. Utilizing a mouse model involving selective ablation ERα liver, we demonstrate that couples lipid metabolism to reproductive cycle. We show regulates transport proteins, enzymes for lipoprotein remodeling, receptors uptake. Additionally, indispensable during proestrus generation high-density lipoproteins...
The incidence and severity of primary cardiac events are inversely related to the plasma concentration high-density lipoproteins (HDLs). We investigated whether HDLs may exert a direct cardioprotection in buffer-perfused isolated rat hearts, which underwent 20-minute low-flow ischemia followed by 30-minute reperfusion. administration at physiological concentrations (0.5 1.0 mg/mL) during 10 minutes immediately before rapidly remarkably improved postischemic functional recovery decreased...
The present study was aimed to evaluate the effect of plant proteins (lupin protein or pea protein) and their combinations with soluble fibres (oat fibre apple pectin) on plasma total LDL-cholesterol levels. A randomised, double-blind, parallel group design followed: after a 4-week run-in period, participants were randomised into seven treatment groups, each consisting twenty-five participants. Each consumed two bars containing specific protein/fibre combinations: reference casein+cellulose;...
The purpose of this study was to investigate whether the expression cellular adhesion molecules (CAMs) is enhanced in individuals with low HDL cholesterol (HDL-C). Plasma levels soluble vascular cell molecule-1 (sVCAM-1), intercellular (sICAM-1), and E-selectin (sE-selectin) were measured subjects (below 10th percentile for Italian population), average, or high (above 90th percentile) HDL-C. Average sICAM-1 sE-selectin significantly higher two groups 65 levels, either hyperlipidemic...
To assess the role of apolipoprotein (apo) E in macrophage reverse cholesterol transport (RCT) vivo.ApoE exerts an antiatherosclerotic activity by regulating lipoprotein metabolism and promoting cell efflux. We discriminated between systemic apoE contribution using assay RCT mice. The complete absence lead to overall impairment process and, similarly, exclusively macrophages resulted reduction mobilization from plasma, liver, feces. Conversely, expression is sufficient promote normal even...
Objective— Aim of this study was to evaluate changes in LCAT (lecithin:cholesterol acyltransferase) concentration and activity patients with an acute coronary syndrome, investigate if these are related the compromised capacity HDL (high-density lipoprotein) promote endothelial nitric oxide (NO) production, assess rhLCAT (recombinant human LCAT) can rescue defective vasoprotective function. Approach Results— Thirty ST-segment–elevation myocardial infarction (STEMI) were enrolled, plasma...
Carriers of the apolipoprotein A-I(Milano) (A-I(M)) variant present with severe reductions plasma HDL levels, not associated premature coronary heart disease (CHD). Sera from 14 A-I(M) carriers and matched controls were compared for their ability to promote ABCA1-driven cholesterol efflux J774 macrophages human fibroblasts. When both cell types are stimulated express ABCA1, through this pathway is greater than control sera (3.4 +/- 1.0% versus 2.3 in macrophages; 5.2 2.4% 1.9 0.1%...
The aim of the present study was to evaluate high-density lipoprotein (HDL) structure and endothelial NO synthase (eNOS) activation capacity in ST-elevation myocardial infarction (STEMI) patients with different acute-phase inflammatory response (APR).Forty-five STEMI were stratified quartiles according delta CRP level, calculated by subtracting value at admission from peak (APR peak). HDL stimulate production evaluated APR peak. a low had completely preserved ability activate eNOS promote...
Lecithin:cholesterol acyltransferase (LCAT) is the enzyme responsible for cholesterol esterification in plasma. LCAT a major factor HDL remodeling and metabolism, it has long been believed to play critical role macrophage reverse transport (RCT). The effect of on human atherogenesis still controversial. In present study, plasma concentration was measured all subjects (n = 540) not drug treatment at time enrollment multicenter, longitudinal, observational IMPROVE study. Mean maximum...
Acute phase reaction (APR) is a systemic inflammation triggered by several conditions associated with lipid profile alterations. We evaluated whether APR also associates changes in cholesterol synthesis and absorption, HDL structure, composition, efflux capacity (CEC). analyzed 59 subjects related to infections, oncologic causes, or autoimmune diseases 39 controls. detected no difference markers of absorption. Conversely, significant reduction LpA-I- LpAI:AII-containing (-28% -44.8%,...
Lipid accumulation in hepatocytes is reduced by the activation of peroxisome proliferator-activated receptor (PPAR) α, which associated with increased lysosomal acid lipase (LAL) activity, transcription factor EB (TFEB) expression, and mitochondrial β-oxidation.Aim study was to assess whether three isoforms PPAR, i.e. δ γ, share same ability reduce lipid clarify involvement autophagy activation, hydrolysis, β-oxidation clearance induced PPARs. HepG2 cells were treated oleate/palmitate (O/P)...
Apolipoprotein A-I (apoA-I) mimetic peptides may represent an alternative to apoA-I for large-scale production of synthetic high-density lipoproteins (sHDL) as a therapeutic agent. In this study, the cardioprotective activity sHDL made with either L37pA peptide or its d-stereoisomer, D37pA, was compared apoA-I. The were reconstituted palmitoyl-oleoyl-phosphatidylcholine, which yielded particles comparable in diameter, molecular weight, and α-helical content. Pretreatment endothelial cells...
Mutations in the CETP gene resulting defective activity have been shown to cause remarkable elevations of plasma HDL-C levels, with accumulation large, buoyant HDL particles enriched apolipoprotein E. Genetic deficiency thus represents a unique tool evaluate how structural alterations impact on atheroprotective functions. Aim present study was assess ability obtained from CETP-deficient subjects protect endothelial cells development dysfunction. isolated one homozygous and seven heterozygous...
Recent evidence suggests that oxidative stress can play a role in the pathogenesis and progression of prostate cancer (PCa). Reactive oxygen species (ROS) generation is higher PCa cells compared to normal epithelial this increase proportional aggressiveness phenotype. Since high density lipoproteins (HDL) are known exert antioxidant activities, their ability reduce ROS levels consequent impact on cell proliferation was tested lines. HDL significantly reduced basal H2O2-induced normal,...