A5089986964- Neonatal Respiratory Health Research
- Tissue Engineering and Regenerative Medicine
- Xenotransplantation and immune response
- Congenital Diaphragmatic Hernia Studies
- Transplantation: Methods and Outcomes
- Electrospun Nanofibers in Biomedical Applications
- Pulmonary Hypertension Research and Treatments
- Organ Transplantation Techniques and Outcomes
- Respiratory Support and Mechanisms
- Mesenchymal stem cell research
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Anesthesia and Neurotoxicity Research
- Heme Oxygenase-1 and Carbon Monoxide
- Infant Nutrition and Health
- Muscle Physiology and Disorders
- Clinical Nutrition and Gastroenterology
- Virus-based gene therapy research
- Cardiac Structural Anomalies and Repair
- Congenital heart defects research
- Organ and Tissue Transplantation Research
- Congenital Heart Disease Studies
- Congenital Anomalies and Fetal Surgery
- Esophageal Cancer Research and Treatment
- Pluripotent Stem Cells Research
- Mitochondrial Function and Pathology
University of Padua
2013-2023
Città della Speranza Foundation
2013
European Society for Organ Transplantation
2004-2011
Consorzio per la Ricerca Sanitaria
2004-2008
Istituto Zooprofilattico Sperimentale delle Venezie
2004
University of Sheffield
2004
Northwick Park Institute for Medical Research
2004
Mesenchymal stem cells (MSCs) prevent the onset of bronchopulmonary dysplasia (BPD) in animal models, an effect that seems to be mediated by their secreted extracellular vesicles (EVs). The aim this study was compare protective effects intratracheally (IT) administered MSCs versus MSC-EVs a hyperoxia-induced rat model BPD. At birth, rats were distributed as follows: animals raised ambient air for 2 wk ( n = 10), and exposed 60% oxygen treated with IT-administered physiological solution or...
Early therapeutic effect of intratracheally (IT)-administered extracellular vesicles secreted by mesenchymal stem cells (MSC-EVs) has been demonstrated in a rat model bronchopulmonary dysplasia (BPD) involving hyperoxia exposure the first 2 postnatal weeks. The aim this study was to evaluate protective effects IT-administered MSC-EVs long term. EVs were produced from MSCs following GMP standards. At birth, rats distributed three groups: (a) animals raised ambient air for 6 weeks (n = 10);...
Muscle tissue engineering can provide support to large congenital skeletal muscle defects using scaffolds able allow cell migration, proliferation and differentiation. Acellular extracellular matrix (ECM) scaffold generate a positive inflammatory response through the activation of anti-inflammatory T-cell populations M2 polarized macrophages that together lead local pro-regenerative environment. This immunoregulatory effect is maintained when acellular matrices are transplanted in xenogeneic...
Abstract Background Treatment of bronchopulmonary dysplasia (BPD) remains as yet an unmet clinical need and recently stem cells have been proposed a therapeutic tool in animal models. We investigated the role amniotic fluid (AFS) adult rat model hyperoxia lung injury. Methods Fifty Sprague–Dawley rats were, at birth, randomly exposed to moderate or room air for 14 days single dose human (hAFS) Fibroblasts (hF), was delivered intratracheally (P21). At P42 animals were euthanized tissue...
The study aimed to evaluate the efficacy of porcine small intestine submucosa extracellular matrix (SIS-ECM) in a murine model, as possible vascular patch for clinical use reconstructive and potentially cardiac surgery.Fifteen adult male Sprague Dawley rats five green fluorescent protein (GFP) were enrolled this study. SIS-ECM graft (6 mm long, 4 wide) was implanted plasty on abdominal aorta animal, after excising part its anterior wall. Histology immunohistochemistry used results at 15, 30,...
Skeletal muscle tissue engineering (TE) aims to efficiently repair large congenital and acquired defects. Biological acellular scaffolds are considered a good tool for TE, as decellularization allows structural preservation of extracellular matrix (ECM) conservation its unique cytokine reservoir the ability support angiogenesis, cell viability, proliferation. This represents major advantage compared synthetic scaffolds, which can acquire these features only after modification show limited...
Congenital diaphragmatic hernia (CDH) is a neonatal defect in which the diaphragm muscle does not develop properly, thereby raising abdominal organs into thoracic cavity and impeding lung development function. Large defects require correction with prosthetic patches to close malformation. This treatment leads consequent generation of unwelcomed mechanical stress repaired recurrences, resulting high morbidity significant mortality rates. We proposed specific diaphragm-derived extracellular...
Hydrogels are biomaterials that, thanks to their unique hydrophilic and biomimetic characteristics, used support cell growth attachment promote tissue regeneration. The use of decellularized extracellular matrix (dECM) from different tissues or organs significantly demonstrated be far superior other types hydrogel since it recapitulates the native tissue’s ECM composition bioactivity. Different muscle injuries malformations require application patches fillers replenish defect boost Herein,...
<h2>Abstract</h2> The aim of this study was to analyze the incidence ureteral stenosis in a life-supporting human decay-accelerating factor (hDAF) transgenic pig-to-cynomolgus monkey kidney transplantation model and determine role possible immunological events its pathogenesis. Thirty consecutive bi-nephrectomized cynomolgus monkeys received from hDAF pigs with or without stent. Four were euthanized prematurely after transplantation. In remaining 26 cases, mean survival 24 ± 19 days. Except...
Abstract Oxidative stress and fibrosis are important responses that characterize bronchopulmonary dysplasia (BPD), a disease for which only therapy but not cure has been developed. In this work, we investigated the effects of mesenchymal stromal cells-derived extracellular vesicles (MSC-EVs) on lung brain compartment in an animal model hyperoxia-induced BPD. Rat pups were intratracheally injected with MSC-EVs produced by human umbilical cord-derived MSC, following Good Manufacturing...
Premature newborns are frequently exposed to hyperoxic conditions and experimental data indicate modulation of liver metabolism by hyperoxia in the first postnatal period. Conversely, nothing is known about possible growth factors signaling molecules involved other responses no available effects haematopoiesis. The aim study was analyse tissue (hepatocytes haemopoietic cells) investigate changes expression Vascular Endothelial Growth Factor (VEGF), Matrix Metalloproteinase 9 (MMP-9),...
Hyperinsulinemia could have a role in the growing incidence of esophageal adenocarcinoma (EAC) and its pre-cancerous lesion, Barrett's Esophagus, possible consequence Gastro-Esophageal Reflux Disease. Obesity is known to mediate carcinogenesis through different mechanisms including insulin-resistance leading hyperinsulinemia, which may cancer progression via insulin/insulin-like growth factor axis. We used hyperinsulinemic non-obese FVB/N (Friend leukemia virus B strain) MKR (muscle...
No papers are available about potentiality of fractal analysis in quantitative assessment alveolarization bronchopulmonary dysplasia (BPD). Thus, we here performed a comparative between [fractal dimension (D) and lacunarity] stereological [mean linear intercept (Lm), total volume alveolar air spaces, number alveoli, mean volume, surface area septa, septal thickness] parameters experimental hyperoxia-induced models BPD. At birth, rats were distributed the following groups: 1) raised ambient...
The production of skeletal muscle constructs useful for replacing large defects in vivo, such as congenital diaphragmatic hernia (CDH), is still considered a challenge. standard application prosthetic material presents major limitations, recurrences remarkable number CDH patients. With this work, we developed tissue engineering approach based on decellularized and human cells the vitro generation diaphragmatic-like tissues proof-of-concept new option surgical treatment diaphragm defects. A...
Abstract Stem cell niche refers to the microenvironment where stem cells reside in living organisms. Several elements define and regulate characteristics, such as stromal support cells, gap junctions, soluble factors, extracellular matrix proteins, blood vessels neural inputs. In last years, different studies demonstrated presence of cKit + human murine amniotic fluid, which have been defined fluid (AFS) cells. Firstly, we characterized present both amnion. Secondly, analyze AFS...
Objectives: The aim of this study was to investigate the contribution host-derived circulating cells cardiac repair after tissue damage using model heterotopic heart transplantation between transgenic recipient rats expressing green fluorescent protein (GFP) and wild-type donors.
Abstract Hamster‐to‐rat heterotopic cardiac xenotransplantation is widely used as an experimental model to study xenograft rejection, accommodation, and tolerance, well in studies aimed at developing immunosuppressive strategies xenotransplantation. Despite its widespread application, no detailed description of a surgical technique for this has been provided the literature. Indeed, all publications so far on use species combination refer rat allotransplantation technique. Hence present paper...
Cardiomyocytes expressing host markers, such as the Y chromosome in sex-mismatched transplants, have been described human allografts, suggesting that circulating cells can contribute to cardiac regeneration. It has not established, however, whether host-derived cardiomyocytes result from transdifferentiation of stem or cell fusion. To address this issue, we used heterotopic heart xenografts and looked for markers donor recipient cells. Golden Syrian hamsters transgenic mice nuclear...
Aim of the study: The lung architecture newborns appears to be affected by an inflammatory reaction maternal choriodecidual layer infection. L-citrulline (L-Cit) was administered pregnant rats exposed intra-amniotic lipopolysaccharide (LPS)-induced chorioamnionitis investigate its effect on neonatal injury. Materials and Methods: pups were assigned four experimental groups: 1- NaCl but not postnatal L-Cit (Controls); 2 - as well treatment (L-Cit group); 3 prenatal LPS (LPS); 4- (LPS +...
Abstract: Methotrexate (MTX) has been used successfully as an immunosuppressant in rodent xenotransplantation models, but the data generated so far with MTX pig‐to‐baboon cardiac transplantation studies have disappointing. The potential of this agent was consequently explored a life‐supporting pig‐to‐primate renal model using cynomolgus monkey recipient species. Introductory vitro and vivo pharmacokinetic pharmacodynamic were conducted three monkeys. Subsequently,10 recipients kidney from...
Apoptotic phenomena observed in vitro following isolation and transplantation contribute significantly to islet graft loss. Strategies reduce apoptosis of tissue prior posttransplantation may improve survival function the amount necessary achieve insulin independence. The expression cytoprotective proteins is one such strategy that prolong survival. In this light, heme-oxygenase 1 (HO-1) upregulation has been studied both allo- xenotransplantation models. study, effect HO-1 on neonatal...