A5011610277- Nicotinic Acetylcholine Receptors Study
- Cholinesterase and Neurodegenerative Diseases
- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Chemical synthesis and alkaloids
- Receptor Mechanisms and Signaling
- Telomeres, Telomerase, and Senescence
- Cellular transport and secretion
- Genetics, Aging, and Longevity in Model Organisms
- Computational Drug Discovery Methods
- Neuroscience and Neural Engineering
- Neurogenesis and neuroplasticity mechanisms
- Pain Mechanisms and Treatments
- Calcium signaling and nucleotide metabolism
- Neuroinflammation and Neurodegeneration Mechanisms
- Genomics and Chromatin Dynamics
- Mercury impact and mitigation studies
- RNA Research and Splicing
- Single-cell and spatial transcriptomics
- Ion Channels and Receptors
- Vagus Nerve Stimulation Research
- Stress Responses and Cortisol
- MicroRNA in disease regulation
- Autism Spectrum Disorder Research
- interferon and immune responses
Instituto Cajal
2022-2024
Universidad Autónoma de Madrid
2009-2022
Hospital Universitario de La Princesa
2018
University of Castilla-La Mancha
2018
Calcium (Ca(2+))-dependent endocytosis has been linked to preferential Ca(2+) entry through the L-type (α(1D), Ca(V)1.3) of voltage-dependent channels (VDCCs). Considering that Ca(2+)-dependent exocytotic release neurotransmitters is mostly triggered by N-(α(1B), Ca(V)2.2) or PQ-VDCCs (α(1A), Ca(V)2.1) and exocytosis are coupled, supposition different channel subtypes specialized control cell functions attractive. Here we have explored this hypothesis in primary cultures bovine adrenal...
Amount evidence indicates that α7 nicotinic acetylcholine receptor (nAChRα7) activation reduces production of inflammatory mediators. This work aimed to verify the influence endogenous nAChRα7 on regulation full-blown muscular inflammation in mdx mouse with Duchenne dystrophy. We used mice 3 weeks-old at height myonecrosis, and C57 nAChRα7+/+ wild-type nAChRα7−/− knockout injury induced 60 µL 0.5% bupivacaine (bp) gastrocnemius muscle. Pharmacological treatment included selective agonist...
A series of multitarget directed propargylamines, as well other differently susbstituted piperidines have been screened potential modulators neuronal nicotinic acetylcholine receptors (nAChRs). Most them showed antagonist actions on α7 nAChRs. Especially, compounds 13, 26, and 38 displayed submicromolar IC50 values homomeric nAChRs, whereas they were less effective heteromeric α3β4 α4β2 nAChRs (up to 20-fold higher in the case 13). Antagonism was concentration dependent noncompetitive,...
Plant extracts of Eugenia punicifolia (Kunth) DC., Myrtaceae, are used in Amazon region Brazil to treat diarrhea and stomach disturbances, as hypoglycemic medicine. We have recently shown that an aqueous extract E. augmented cholinergic neurotransmission a rat phrenic nerve-diaphragm preparation. In this study, we investigated the effects dichloromethane (EPEX) neuronal model neurotransmission, bovine adrenal chromaffin cell. EPEX release catecholamine triggered by acetylcholine (ACh) pulses...
The inhibition of nicotinic acetylcholine receptors (nAChRs) has been proposed as a potential strategy to develop new antidepressant drugs. This is based on the observation that antidepressants selectively block noradrenaline (NA) or serotonin (5-HT) reuptake also inhibit nAChRs. Dual blocking both NA and 5-HT were shorten delay in exerting their clinical effects; whether duloxetine, prototype dual antidepressants, blocks nAChRs unknown. Here we explored this question bovine chromaffin cells...
Alzheimer´s disease (AD) is characterized by neuronal function loss and degeneration. The integrity of the axon initial segment (AIS) essential to maintain output. AIS alterations are detected in human post-mortem AD brains mice models, as well as, neurodevelopmental mental disorders. However, mechanisms leading deregulation extrinsic glial origin elusive. We studied early postnatal differences cellular/molecular wild-type or APP/PS1 combined neuron-astrocyte co-cultures. observed...
Alzheimer’s disease (AD), the most common form of dementia, is becoming a global health problem and public priority. In advanced stages AD, besides initial cognitive symptoms, behavioral problems, particularly agitation aggressiveness, become prevalent in AD patients. These non-cognitive symptoms could be related to noradrenergic overactivation. this study, we used chromaffin cells (CCs) isolated from adrenal gland 3xTg model mice characterize potential alterations autocrine-paracrine...