A5089168647- Neurogenesis and neuroplasticity mechanisms
- Pluripotent Stem Cells Research
- Nerve injury and regeneration
- Neuroinflammation and Neurodegeneration Mechanisms
- Metabolism and Genetic Disorders
- Lysosomal Storage Disorders Research
- Growth Hormone and Insulin-like Growth Factors
- Developmental Biology and Gene Regulation
- Nuclear Receptors and Signaling
- Diet and metabolism studies
- Neuroscience and Neuropharmacology Research
- Adipose Tissue and Metabolism
- Anesthesia and Neurotoxicity Research
- Cellular transport and secretion
- Mesenchymal stem cell research
- Axon Guidance and Neuronal Signaling
- Autophagy in Disease and Therapy
- Mitochondrial Function and Pathology
- MicroRNA in disease regulation
- Alzheimer's disease research and treatments
- Pancreatic function and diabetes
- Diabetic Foot Ulcer Assessment and Management
- Parkinson's Disease Mechanisms and Treatments
- Spine and Intervertebral Disc Pathology
- RNA Interference and Gene Delivery
Biomedical Research Networking Center on Neurodegenerative Diseases
2014-2024
Instituto Cajal
2014-2024
Consejo Superior de Investigaciones Científicas
2013-2024
Instituto de Salud Carlos III
2009-2024
Centro de Investigación Biomédica en Red
2022-2023
Centro Científico Tecnólogico - Rosario
2017
Center for Biomedical Research of La Rioja
2012
Hospital General Nuestra Señora del Prado
2007
Hospital Clínico San Carlos
2003-2007
Centro de Investigaciones Biológicas Margarita Salas
2004-2006
Cell cultures were used to analyze the role of brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) in development synaptic transmission. Neurons obtained from embryonic day 18 (E18) rat hippocampus cultured for 2 weeks exhibited extensive spontaneous activity. By comparison, neurons E16 expressed very low levels or evoked Neurotrophin treatment produced a sevenfold increase number functional connections cultures. BDNF induced formation both excitatory inhibitory synapses,...
Mammalian cells harbor three highly homologous and widely expressed members of the ras family (H-ras, N-ras, K-ras), but it remains unclear whether they play specific or overlapping cellular roles. To gain insight into such functional roles, here we generated analyzed H-ras null mutant mice, which were then also bred with N-ras knockout animals to ascertain viability properties potential double mutations in both loci. Mating among heterozygous H-ras(+/-) mice produced H-ras(-/-) offspring a...
Abstract Background Heterozygous mutations in the GBA1 gene, which encodes lysosomal enzyme β‐glucocerebrosidase‐1, increase risk of developing Parkinson's disease, although underlying mechanisms remain unclear. The aim this study was to explore impact N370S ‐GBA1 mutation on cellular homeostasis and vulnerability a patient‐specific model PD. Methods We isolated fibroblasts from 4 PD patients carrying N370S/wild type 6 controls autophagy‐lysosome pathway, endoplasmic reticulum stress, Golgi...
After late embryogenesis, new neurons are continuously added to the olfactory bulb (OB) from stem cells located in forebrain subventricular zone. Nonetheless, have not been described within embryonic bulb. Here we report isolation of local day 12.5–14.5 mouse embryo. These were 99.2% nestin positive and proliferated extensively culture at least 150 cell doublings. Clonal analysis demonstrated that (TuJ1 + ), astrocytes (GFAP oligodendrocytes (O4 ) could be generated single-plated cells,...
Neural stem cells depend on insulin-like growth factor I (IGF-I) for differentiation. We analysed how activation and inhibition of the PI 3-kinase–Akt signalling affects number differentiation mouse olfactory bulb (OBSCs). Stimulation pathway with insulin and/or IGF-I, led to an increase in Akt phosphorylated residues Ser473 Thr308 (P-AktSer473 P-AktThr308, respectively) proliferating OBSCs, differentiating cells. Conversely, P-AktSer473 levels decreased by 50% OB embryonic day 16.5-18.5...
Abstract While insulin‐like growth factor‐I (IGF‐I) supports neuronal and glial differentiation in the CNS, it is largely unknown whether IGF‐I also influences migration positioning. We show here that pattern of olfactory bulb (OB) layering altered Igf‐I −/− mice. In these animals, Tbr1 + ‐glutamatergic neurons are misplaced mitral cell layer (ML) external plexiform (EPL). addition, there fewer interneurons glomerular EPL mice, newborn incorporated into OB from forebrain subventricular zone...
Lipid and cholesterol metabolism might play a role in the pathogenesis of Parkinson disease (PD). However, association between PD is not clearly established. Cholesterol accumulation closely related to expression multilamellar bodies (MLBs). Also, controls autophagosome transport. Thus, impaired trafficking lead robust autophagic vacuole accumulation. Our recent work provides first evidence that presence N370S GBA mutation produces an cholesterol, which alters autophagy-lysosome function...
During the development of CNS, a salient issue is whether neuronal phenotype defined by lineage or environment precursor cells. Transplants permit these two possibilities to be tested, as cell fate can examined in new location. Dissociated cerebellar cells from newborn rats treated with tritiated thymidine NSE-lacZ transgenic mice were grafted into dentate gyrus developing hippocampus. Implanted integrated granule layer, which contains bodies host neurons. Immunohistochemistry showed that...
Methamphetamine (METH), a commonly abused psychostimulant, causes dopamine neurotoxicity in humans, rodents, and nonhuman primates. This study examined the selective neuroanatomical pattern of dopaminergic induced by METH mouse striatum. We effect on tyrosine hydroxylase (TH) transporter (DAT) immunoreactivity different compartments striatum nucleus accumbens. The levels its metabolites, 3,4-dihidroxyphenylacetic acid homovanillic acid, as well serotonin (5-HT) metabolite,...
Abstract Stem cell technology promises new and rapid advances in therapy drug discovery. Clearly, the value of this approach will be limited by differentiated functions displayed progeny stem cells. The foetal adult central nervous system (CNS) harbour cells that can expanded vitro differentiate into immature neurons glia. Surprisingly, we do not know if derived from form synapses, a definitive feature neuronal function. Neuronal differentiation is complex process paper establish conditions...
We have recently demonstrated that embryonic E16 hippocampal neurons grown in cultures are unable to form fast synaptic connections unless treated with BDNF or NT-3. This experimental system offers an opportunity define the roles of neurotrophins processes leading formation functional connections. used ultrastructural and electrophysiological methods explore cellular locations underlying neurotrophin action on maturation. The rate spontaneous miniature excitatory postsynaptic currents...
Abstract : The utilization of lactate, glucose, 3‐hydroxybutyrate, and glutamine has been studied in isolated brain cells from early newborn rats. Isolated actively utilized these substrates, showing saturation at concentrations near physiological levels during the perinatal period. rate lactate was 2.5‐fold greater than that observed for or glutamine, suggesting is main metabolic substrate immediately after birth. apparent K m glucose suggested this process limited by activity hexokinase....
Abstract Cell lineages generated during development and tissue maintenance are derived from self-renewing stem cells by differentiation of their committed progeny. Recent studies suggest that epigenetic mechanisms, in particular the Polycomb group (PcG) genes, play important roles controlling cell self-renewal. Here, we address PcG regulation self-renewal through inactivation Ring1B, a histone H2A E3 monoubiquitin ligase, embryonic neural (NSCs) olfactory bulb conditional mouse mutant line....
The specific actions of insulin-like growth factor-I (IGF-I) and the role brain-derived IGF-I during hippocampal neurogenesis have not been fully defined. To address influence on stages neurogenesis, we studied a postnatal/adult global Igf-I knockout (KO) mice (Igf-I(-/-) ) nervous system conditional KO (Igf-I(Δ/Δ) ). In both found an accumulation Tbr2(+) -intermediate neuronal progenitors, some which were displaced in outer granule cell layer (GCL) molecular (ML) dentate gyrus (DG)....
During the embryonic period, many olfactory bulb (OB) interneurons arise in lateral ganglionic eminence (LGE) from precursor cells expressing Dlx2,Gsh2 and Er81 transcription factors. Whether GABAergic dopaminergic are also generated within OB has not been studied thoroughly. In contrast to abundant Dlx2 Gsh2 expression eminences (GE), proteins expressed E12.5-13.5 mouse OB, whereas telencephalic pallial domain marker Pax6 is abundant. We found neurons originating dividing E13.5 short-term...
The paired type homeobox 6 (Pax6) transcription factor (TF) regulates multiple aspects of neural stem cell (NSC) and neuron development in the embryonic central nervous system. However, less is known about role Pax6 maintenance differentiation adult NSCs neurogenesis. Using +/Sey(Dey) mouse, we have analyzed how heterozygosis influences self-renewal proliferation olfactory bulb cells (aOBSCs). In addition, assessed its influence on differentiation, neuronal incorporation, death OB, both vivo...