A5056913678- Cancer-related molecular mechanisms research
- Neuroscience and Neuropharmacology Research
- RNA Research and Splicing
- Neuroinflammation and Neurodegeneration Mechanisms
- Receptor Mechanisms and Signaling
- Adipose Tissue and Metabolism
- Cannabis and Cannabinoid Research
- Signaling Pathways in Disease
- Circular RNAs in diseases
- Nuclear Receptors and Signaling
- MicroRNA in disease regulation
- Histone Deacetylase Inhibitors Research
- Microtubule and mitosis dynamics
- RNA modifications and cancer
- Memory and Neural Mechanisms
- Exercise and Physiological Responses
- Retinal Development and Disorders
- Mitochondrial Function and Pathology
- Biochemical Acid Research Studies
- Sleep and Wakefulness Research
- Lysosomal Storage Disorders Research
- RNA regulation and disease
- Autophagy in Disease and Therapy
- Muscle Physiology and Disorders
- Cardiovascular Effects of Exercise
Centro Andaluz de Biología Molecular y Medicina Regenerativa
2023-2025
Universidad Pablo de Olavide
2023-2025
Universidad de Sevilla
2023-2025
Consejo Superior de Investigaciones Científicas
2010-2024
Scripps Institution of Oceanography
2021-2024
The Herbert Wertheim UF Scripps Institute for Biomedical Innovation & Technology
2023-2024
Scripps (United States)
2021-2024
Scripps Research Institute
2019-2021
Instituto Cajal
2010-2020
Instituto de Salud Carlos III
2010-2020
Associative learning depends on multiple cortical and subcortical structures, including striatum, hippocampus, amygdala. Both glutamatergic dopaminergic neurotransmitter systems have been implicated in memory consolidation. While the role of glutamate is well established, dopamine its receptors these processes less clear. In this study, we used two models D(1) receptor (D(1)R, Drd1a) loss, D(1)R knock-out mice (Drd1a(-/-)) with intrahippocampal injections Drd1a-siRNA (small interfering RNA),...
Abstract Background Heterozygous mutations in the GBA1 gene, which encodes lysosomal enzyme β‐glucocerebrosidase‐1, increase risk of developing Parkinson's disease, although underlying mechanisms remain unclear. The aim this study was to explore impact N370S ‐GBA1 mutation on cellular homeostasis and vulnerability a patient‐specific model PD. Methods We isolated fibroblasts from 4 PD patients carrying N370S/wild type 6 controls autophagy‐lysosome pathway, endoplasmic reticulum stress, Golgi...
An lncRNA is synaptically transported in a cAMP-dependent manner and linked to cytoskeletal components of structural plasticity.
Long noncoding RNAs (lncRNAs) play crucial roles in maintaining cell homeostasis and function. However, it remains largely unknown whether how neuronal activity impacts the transcriptional regulation of lncRNAs, or if this leads to synapse-related changes contributes formation long-term memories. Here, we report identification a lncRNA, SLAMR, which becomes enriched CA1-hippocampal neurons upon contextual fear conditioning but not CA3 neurons. SLAMR is transported along dendrites via...
Abstract Dopamine receptors play an important role in motivational, emotional, and motor responses. In addition, growing evidence suggests a key of hippocampal dopamine learning memory. It is well known that associative synaptic plasticity CA3-CA1 requires the D1 receptor (D1R). However, specific D2 (D2R) on memory-related neuroplasticity processes still undefined. Here, by using two models D2R loss, knockout mice (Drd2−/−) with intrahippocampal injections Drd2-small interfering RNA...
The antioxidant and CB2 receptor agonist properties of Δ9-tetrahydrocannabivarin (Δ9-THCV) afforded neuroprotection in experimental Parkinson's disease (PD), whereas its CB1 antagonist profile at doses lower than 5 mg/kg caused anti-hypokinetic effects. In the present study, we investigated anti-dyskinetic potential Δ9-THCV (administered i.p. 2 for two weeks), which had not been before. This objective was after inducing dyskinesia by repeated administration L-DOPA (i.p. 10 mg/kg) a genetic...
Abstract ATP-citrate lyase is a central integrator of cellular metabolism in the interface protein, carbohydrate, and lipid metabolism. The physiological consequences as well molecular mechanisms orchestrating response to long-term pharmacologically induced Acly inhibition are unknown. We report here that inhibitor SB-204990 improves metabolic health physical strength wild-type mice when fed with high-fat diet, while healthy diet results imbalance moderated insulin resistance. By applying...
Synaptic structural plasticity, key to long-term memory storage, requires translation of localized RNAs delivered by long-distance transport from the neuronal cell body. Mechanisms and regulation this system remain elusive. Here, we explore roles KIF5C KIF3A, two members kinesin superfamily molecular motors (Kifs), find that loss function either decreases dendritic arborization spine density whereas gain enhances it. is a rate-determining component local associated with ∼650 RNAs, including...
Abstract Plakophilin-2 (PKP2) is a key component of desmosomes, which, when defective, known to promote the fibro-fatty infiltration heart muscle. Less attention has been given its role in adipose tissue. We report here that levels PKP2 steadily increase during fat cell differentiation, and are compromised if adipocytes exposed pro-inflammatory milieu. Accordingly, expression subcutaneous tissue diminishes patients with obesity, normalizes upon mild-to-intense weight loss. further show...
We studied, in behaving mice, the contribution of CB1 receptors to activity-dependent changes induced at hippocampal CA3-CA1 synapse by associative learning and following experimentally evoked long-term potentiation (LTP). Mice were classically conditioned evoke eyelid responses with a trace paradigm using tone as stimulus (CS) an electric shock unconditioned (US). Field excitatory postsynaptic potentials (fEPSPs) during CS-US interval across training. Conditioning was performed presence...
ATP citrate lyase (ACLY) inhibitors have the potential of modulating central processes in protein, carbohydrate, and lipid metabolism, which can relevant physiological consequences aging age-related diseases. Here, we show that hepatic phospho-active ACLY correlates with overweight Model for End-stage Liver Disease score humans. Wild-type mice treated chronically inhibitor potassium hydroxycitrate exhibited delayed early mortality. In AML12 hepatocyte cultures, hydroxycitrate, SB-204990,...
Abstract Molecular and cellular mechanisms underlying the role of prelimbic cortex in contextual fear memory remain elusive. Here we examined kinesin family molecular motor proteins (KIFs) for their mediating fear, a form associative memory. KIFs function as critical mediators synaptic transmission plasticity by ability to modulate microtubule transport gene products. However, regulation insofar consolidation is not known. We find that within one hour conditioning, expression KIF3B...
Abstract Activity-dependent changes in neuronal connections are fundamental to learning and long-term memory storage. However, the precise contribution of long noncoding RNAs (lncRNAs) these modifications remains unclear. In this study, we assessed role lncRNA ADEPTR, a cAMP-modulated localized dendrites, which is crucial for synapse morphology. By generating two different mouse models—one with deletion ADEPTR (L-ADEPTR) one its protein interaction region (S-ADEPTR)—we investigated...
Abstract LncRNAs are involved in critical processes for cell homeostasis and function. However, it remains largely unknown whether how the transcriptional regulation of long noncoding RNAs results activity-dependent changes at synapse facilitate formation long-term memories. Here, we report identification a novel lncRNA, SLAMR, that becomes enriched CA1- but not CA3-hippocampal neurons upon contextual fear conditioning. SLAMR is transported to dendrites via molecular motor KIF5C recruited...
Abstract Activity-dependent structural plasticity at the synapse requires specific changes in neuronal transcriptome. While much is known about role of coding elements this process, long-noncoding transcriptome remains elusive. Here we report discovery an intronic long noncoding RNA (lncRNA)—termed ADEPTR—whose expression upregulated and synaptically transported a cAMP/PKA-dependent manner hippocampal neurons, independent its protein-coding host gene. Loss ADEPTR function suppresses...
Synaptic structural plasticity, a key component of long-term memory storage, requires translation localized RNAs delivered by long-distance transport from the neuronal cell body. However,the mechanisms and regulation this process that ensures plasticity remain elusive. Here,we explored roles Kif5C Kif3A, two members Kinesin superfamily machinery (Kifs), foundthat loss function either onedecreased plasticity,whereas gain enhanced it. Importantly, is rate-determining local mediates ~650 RNAs,...