A5079544290- Cellular transport and secretion
- Parkinson's Disease Mechanisms and Treatments
- Autophagy in Disease and Therapy
- Neurotransmitter Receptor Influence on Behavior
- Receptor Mechanisms and Signaling
- Neuroscience and Neuropharmacology Research
- Lysosomal Storage Disorders Research
- Gestational Diabetes Research and Management
- Congenital heart defects research
- Epigenetics and DNA Methylation
- Pancreatic function and diabetes
- Biomedical and Engineering Education
- Neurological disorders and treatments
- Health and Medical Research Impacts
- RNA modifications and cancer
- Health Systems, Economic Evaluations, Quality of Life
- Pluripotent Stem Cells Research
- Botulinum Toxin and Related Neurological Disorders
- Adenosine and Purinergic Signaling
- Folate and B Vitamins Research
- RNA Interference and Gene Delivery
- Cancer-related molecular mechanisms research
- Cannabis and Cannabinoid Research
- Lung Cancer Diagnosis and Treatment
- Nuclear Receptors and Signaling
Consejo Superior de Investigaciones Científicas
2010-2023
Instituto de Salud Carlos III
2013-2023
Biomedical Research Networking Center on Neurodegenerative Diseases
2017-2023
Instituto Cajal
2013-2023
Centro de Investigación Biomédica en Red
2020-2023
Agencia de Evaluación de Tecnologías Sanitarias
2023
Digital Research Alliance of Canada
2023
Precision for Medicine (United States)
2023
Fundación Progreso y Salud
2023
Andalusian Health Service
2023
Abstract Background Heterozygous mutations in the GBA1 gene, which encodes lysosomal enzyme β‐glucocerebrosidase‐1, increase risk of developing Parkinson's disease, although underlying mechanisms remain unclear. The aim this study was to explore impact N370S ‐GBA1 mutation on cellular homeostasis and vulnerability a patient‐specific model PD. Methods We isolated fibroblasts from 4 PD patients carrying N370S/wild type 6 controls autophagy‐lysosome pathway, endoplasmic reticulum stress, Golgi...
Lipid and cholesterol metabolism might play a role in the pathogenesis of Parkinson disease (PD). However, association between PD is not clearly established. Cholesterol accumulation closely related to expression multilamellar bodies (MLBs). Also, controls autophagosome transport. Thus, impaired trafficking lead robust autophagic vacuole accumulation. Our recent work provides first evidence that presence N370S GBA mutation produces an cholesterol, which alters autophagy-lysosome function...
Adenosine A2A receptors (A2AR) are located postsynaptically in striatopallidal GABAergic neurons, antagonizing dopamine D2 receptor functions, and also presynaptically at corticostriatal terminals, facilitating glutamate release. To address the hypothesis that these two A2AR populations differently control action of psychostimulants, we characterized modulation cocaine-induced effects level DARPP-32 phosphorylation Thr-34 Thr-75, c-Fos expression, psychomotor activity using lines cell-type...
Abstract Dopamine receptors play an important role in motivational, emotional, and motor responses. In addition, growing evidence suggests a key of hippocampal dopamine learning memory. It is well known that associative synaptic plasticity CA3-CA1 requires the D1 receptor (D1R). However, specific D2 (D2R) on memory-related neuroplasticity processes still undefined. Here, by using two models D2R loss, knockout mice (Drd2−/−) with intrahippocampal injections Drd2-small interfering RNA...
Introduction Alzheimer’s disease remains the most common neurodegenerative disorder, depicted mainly by memory loss and presence in brain of senile plaques neurofibrillary tangles. This is related to several cellular alterations like synapses, neuronal death, disruption lipid homeostasis, mitochondrial fragmentation, or raised oxidative stress. Notably, changes autophagic pathway have turned out be a key factor early development disease. The aim this research determine impact APOE allele ε4...
Abstract Repeated cocaine exposure causes long‐lasting neuroadaptations that involve alterations in cellular signaling and gene expression mediated by dopamine different brain regions, such as the striatum. Previous studies have pointed out to D1 receptor one major player psychostimulants‐induced behavioral, cellular, molecular changes. However, role of other receptors has not been fully characterized. Here we used D2 knockout (D2 −/− ) mice explore (D2R) behavioral sensitization its...
Abstract Oxidative stress constitutes a major cause for increased risk of congenital malformations associated to severe hyperglycaemia during pregnancy. Mutations in the gene encoding transcription factor ALX3 craniofacial and neural tube defects. Since oxidative lack favour excessive embryonic apoptosis, we investigated whether ALX3-deficiency further increases damage gestational mice. We found that are enhanced diabetic pregnancies. Increased expression genes stress-scavenging enzymes...
During embryonic development, the aristaless-type homeodomain protein Alx3 is expressed in forehead mesenchyme and contributes to regulation of craniofacial development. In adult, pancreatic islets where it participates control glucose homoeostasis. present study, we investigated transcriptional gene expression these two cell types. We found that promoter contains E-box regulatory elements, named EB1 EB2, provide binding sites for basic helix–loop–helix transcription factors Twist1, E47, USF...
Introduction Personalized precision medicine (PPM) is an innovative approach to disease diagnosis, prognosis, and treatment of individual or group characteristics using diverse data sources. While omics technologies are integral PPM, they pose challenges. Therefore, developing appropriate methodology assess these crucial for patient safety, resource efficiency, clinical decision-making within the Spanish National Health System. Methods This health technology assessment (HTA) procedure was...