Phosphorylated ATM immunofluorescence staining was used to investigate the dose-response relationship for number of DNA double-strand breaks (DSBs) induced in primary normal human fibroblasts irradiated with doses from 1.2 200 mGy. The induction DSBs showed a supralinear relationship. Radiation-induced bystander effects may explain these findings. To test this hypothesis, cells treated lindane, an inhibitor radiation-induced effects, prior X irradiation assessed; not observed. Moreover,...

Our previous study suggested that the DNA double-strand breaks (DSBs) induced by very low X-ray doses are largely due to bystander effects. The aim of this was verify whether DSBs created radiation-induced effects likely be repaired. We examined generation in cells enumeration phosphorylated ataxia telangiectasia mutated (ATM) foci, which correlated with DSB repair, normal human fibroblast (MRC-5) after X irradiation at ranging from 1 1000 mGy. At 24 h irradiation, 100% (1.2 mGy), 58% (20...

We examined the delayed induction of telomere instability in hTERT-immortalized normal human fibroblast (BJ1-hTERT) cells exposed to X-rays. BJ1-hTERT were irradiated with 2 Gy X-rays, and chromosome aberrations analyzed 24 hours after irradiation surviving 14 days X-ray exposure. found that X-ray-surviving showed an increased frequency chromatid gaps breaks fragments compared control cells. Furthermore, centromere- telomere-FISH revealed loss duplication significantly level. Because no...

Abstract To clarify the health risks of internal radiation exposure, it is important to investigate radiological effects local exposure at cell levels from radioactive materials taken up by organs. Focusing on response populations post-irradiation, X-ray microbeams are very effective reproducing within an in vitro. The present study aims investigating normal human (MRC-5) irradiated with different beam sizes DNA damage. MRC-5 were locally 1 Gy 0.02–1.89 mm 2 field sizes, and analyzed whether...

Computed tomography (CT) dose calculators such as the WAZA-ARI are useful for estimating radiation from CT examination. This study determined correction coefficients organ doses to patients of any size attended in daily clinical practice. To this end, authors constructed voxel phantoms based on images different and simulated transport examinations obtain using Monte Carlo simulation. The results show that linear relationship between effective diameter can predict patient-specific doses....

Radiation-induced acute myeloid leukemia (rAML) in C3H mice is commonly developed through inactivation of PU.1 transcription factor encoded Sfpi1 on chromosome 2. involves two steps: hemizygous deletion the gene (DSG) and point mutation allele (PMASG). In this study, we investigated dose-rate dependence frequency both DSG PMASG hematopoietic stem cells (HSCs) that received a total 3 Gy gamma-ray exposure at dose rates 20 mGy/day, 200 mGy/day or 1,000 mGy/min. All were followed for 250 days...

We determined the frequencies of dicentric chromosomes per cell in non-dividing confluent normal human fibroblasts (MRC-5) irradiated with a single 1 Gy dose or fractionated (10X0.1 Gy, 5X0.2 and 2X0.5 Gy). The interval between fractions was min to 1440 min. After completion X-irradiation, cells were incubated for 24 hours before re-plating at low density. Then, demecolcine administrated 6 hours, first mitotic collected 42 hours. Our study demonstrated that different significantly reduced if...

Background: A NaI(Tl) survey meter was used in the pediatric thyroid screening conducted after Fukushima Daiichi Nuclear Power Plant accident. However, this measurement method has weakness that it is difficult to selectively identify <sup>131</sup>I. In study, we analyzed performance of an energy-analyzable spectrometer using Particle and Heavy Ion Transport code System (PHITS), which allows Monte Carlo simulation radiation transport.Materials Methods: The spectrum energy emitted...

The effectiveness of skin care to radiation dermatitis (RD) on patients who received radiotherapy for cancer has not been clarified. purpose this study was investigate the effect moisturizers and washing barrier function possibly leading development RD using X-ray irradiated hairless mice.Nine-week-old mice were with 10 ​Gy X-rays, group had applied or soap from day irradiation during observations. condition observed evaluate RD. Skin evaluated by measuring temperature transepidermal water...

The goal of this study was to determine whether in vivo X irradiation induces nontargeted effects, such as delayed effects and bystander ICR mouse lymphocytes. We first examined the generation DNA double-strand breaks (DSBs) lymphocytes, isolated from mice exposed 1 Gy irradiation, by enumeration p53 binding protein (53BP1) foci, observed that number 53BP1 foci reached their maximum 3 days postirradiation decreased background level 30 postirradiation. However, significantly increased...

Purpose Several past studies using a mouse model of radiation-induced AML (rAML) have shown that hemizygous deletion the Sfpi1 gene (HDSG) is an initiating event for development rAML. In this study, we examined difference in frequency HDSG hematopoietic stem cells (HSCs) Rich Cell population (HRCs) from bone marrow (BM) and spleen C3H mice irradiated with 3 Gy X-rays.Materials methods 8-weeks old male were 3Gy whole body X-ray (1 Gy/min) sacrificed at 1, 4, 8, 26 weeks. Then, HSPCs isolated...