A5064464080- DNA Repair Mechanisms
- Effects of Radiation Exposure
- Cancer therapeutics and mechanisms
- Pluripotent Stem Cells Research
- Mitochondrial Function and Pathology
- Cancer, Hypoxia, and Metabolism
- Cellular Mechanics and Interactions
- Congenital heart defects research
- Single-cell and spatial transcriptomics
- Telomeres, Telomerase, and Senescence
- Liver Disease Diagnosis and Treatment
- Lipid metabolism and disorders
- Cancer-related Molecular Pathways
- Carcinogens and Genotoxicity Assessment
- Gout, Hyperuricemia, Uric Acid
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- 3D Printing in Biomedical Research
- Cancer Cells and Metastasis
- Amyotrophic Lateral Sclerosis Research
- Adipose Tissue and Metabolism
- Radiation Therapy and Dosimetry
- ATP Synthase and ATPases Research
- Prostate Cancer Treatment and Research
- DNA and Nucleic Acid Chemistry
- Redox biology and oxidative stress
Nara Medical University
2019-2023
Kyoto University
2019
Oita University
2014-2016
Nagasaki University
2009-2011
The brainstem is a posterior region of the brain, composed three parts, midbrain, pons, and medulla oblongata. It critical in controlling heartbeat, blood pressure, respiration, all which are life-sustaining functions, therefore, damages to or disorders can be lethal. Brain organoids derived from human pluripotent stem cells (hPSCs) recapitulate course brain development expected useful for medical research on central nervous system disorders. However, existing organoid models limited extent...
Radiation-induced cell death is thought to be caused by nuclear DNA damage that cannot repaired. However, in this study we found a delayed increase of mitochondrial reactive oxygen species (ROS) responsible for some the radiation-induced normal human fibroblast cells. We have previously reported there (·)O2(-), measured using MitoSOX™ Red reagent, due gamma irradiation. This dependent on Drp1 localization mitochondria. Here, show knockdown expression reduces level double-strand breaks (DSBs)...
Ischemic stroke is one of the most common neurological diseases. However, impact ischemic on human cerebral tissue remains largely unknown due to a lack brain samples. In this study, we applied organoids derived from induced pluripotent stem cells evaluate effect oxygen-glucose deprivation/reoxygenation (OGD/R). Pathway analysis showed relationships between vitamin digestion and absorption, fat peroxisome proliferator-activated receptor (PPAR) signaling pathway, complement coagulation...
The excretion and reabsorption of uric acid both to from urine are tightly regulated by transporters. Metabolic syndrome conditions, such as obesity, hypercholesterolemia, insulin resistance, believed regulate the expression transporters decrease acid. However, mechanisms driving cholesterol impacts on have been unknown. Here, we show that metabolite 27-hydroxycholesterol (27HC) upregulates transporter URAT1 encoded SLC22A12 via estrogen receptors (ER). Transcriptional motif analysis showed...
Cellular senescence has long been considered to act as a tumor suppressor or suppression mechanism and described phenomenon of irreversible cell cycle arrest. senescence, however, is now have physiological functions other than suppression; it found be involved in embryogenesis, tissue/organ aging, wound healing. Surprisingly, cellular also demonstrated progressive role certain situations. Senescent cells exhibit secretory phenotypes called senescence-associated phenotype (SASP), which...
Proline:arginine (PR) poly-dipeptides from the GGGGCC repeat expansion in C9orf72 have cytotoxicity and bind intermediate filaments (IFs). However, it remains unknown how PR affect cytoskeletal organization focal adhesion (FA) formation. Here, we show that changes to cytoskeleton FA by result alteration of cell stiffness mechanical stress response. increased junctions branches IF network stiffness. They also changed distribution actin size intracellular calcium concentration. or an inhibitor...
The anticancer agent 5-fluorouracil (5-FU) is cytotoxic and often used to treat various cancers. 5-FU thought inhibit the enzyme thymidylate synthase, which plays a role in nucleotide synthesis has been found induce single- double-strand DNA breaks. ATR Ser/Thr kinase (ATR) principal damage response activated UV– chemotherapeutic drug–induced replication stress, but its cellular responses unclear. In this study, we examined effect of inhibition on sensitivity mammalian cells. Using...
We examined mechanisms involved in delayed mutagenesis CHO-LacZeo cells harboring the fusion gene between bacterial LacZ and Zeocin-resistance genes. After X irradiation, Zeocin-resistant primary colonies were isolated, clones subjected to secondary colony formation absence of Zeocin. found that surviving showed a significantly higher mutation frequency compared with those derived from nonirradiated cells. The spectrum was analyzed by gene-specific PCR. more than 90% spontaneous direct...
Abstract Elevated levels of uric acid, a metabolite purine in humans, is related to various diseases, such as gout, atherosclerosis and renal dysfunction. The excretion reabsorption acid to/from urine tightly regulated by transporters. amino sequences transporters, URAT1/ SLC22A12 , OAT4/ SLC22A11 OAT10/ SLC22A13 share closer phylogenic relationship, whereas the gene promoter are distant relationship. Through single-cell RNA-sequencing analysis an adult human kidney, we found that only small...
Summary The brainstem controls heartbeat, blood pressure and respiration, which are life-sustaining functions, therefore, disorders of the can be lethal. Brain organoids derived from human pluripotent stem cells recapitulate course brain development expected to useful for medical research on central nervous system disorders. However, existing organoid models have limitations, hampering elucidation diseases affecting specific components brain. Here, we developed a method generate (hBSOs),...
The goal of this study was to determine whether in vivo X irradiation induces nontargeted effects, such as delayed effects and bystander ICR mouse lymphocytes. We first examined the generation DNA double-strand breaks (DSBs) lymphocytes, isolated from mice exposed 1 Gy irradiation, by enumeration p53 binding protein (53BP1) foci, observed that number 53BP1 foci reached their maximum 3 days postirradiation decreased background level 30 postirradiation. However, significantly increased...
Abstract Amyotrophic lateral sclerosis (ALS) is an irreversible neurodegenerative disease caused by the degeneration of motor neurons, and cytoskeletal instability considered to be involved in neurodegeneration. A hexanucleotide repeat expansion C9orf72 , one most common causes familial ALS, produces toxic proline:arginine (PR) poly-dipeptides. PR poly-dipeptides binds polymeric forms low complexity sequences intracellular puncta, thereby altering intermediate filaments (IFs). However, how...
ABSTRACT The anticancer agent, 5-fluorouracil (5-FU), is typically applied in the treatment of various types cancers because its properties. Thought to be an inhibitor enzyme thymidylate synthase which plays a role nucleotide synthesis, 5-FU has been found induce single- and double-strand DNA breaks. activation ATR occurs as reaction UV- chemotherapeutic drug-induced replication stress. In this study, we examined effect inhibition on sensitivity. Using western blotting, that led...