A5060472191- Pluripotent Stem Cells Research
- Adipose Tissue and Metabolism
- Congenital heart defects research
- Single-cell and spatial transcriptomics
- Nuclear Structure and Function
- RNA Research and Splicing
- Barrier Structure and Function Studies
- Diet and metabolism studies
- RNA and protein synthesis mechanisms
- 3D Printing in Biomedical Research
Nara Medical University
2019-2021
The brainstem is a posterior region of the brain, composed three parts, midbrain, pons, and medulla oblongata. It critical in controlling heartbeat, blood pressure, respiration, all which are life-sustaining functions, therefore, damages to or disorders can be lethal. Brain organoids derived from human pluripotent stem cells (hPSCs) recapitulate course brain development expected useful for medical research on central nervous system disorders. However, existing organoid models limited extent...
Abstract Nuclear import receptors (NIRs) not only transport RNA-binding proteins (RBPs) but also modify phase transitions of RBPs by recognizing nuclear localization signals (NLSs). Toxic arginine-rich poly-dipeptides from C9orf72 interact with NIRs and cause nucleocytoplasmic deficit. However, the molecular basis for toxicity toward function as modifiers remains unidentified. Here we show that impede ability to RBPs. Isothermal titration calorimetry size-exclusion chromatography revealed...
Ischemic stroke is one of the most common neurological diseases. However, impact ischemic on human cerebral tissue remains largely unknown due to a lack brain samples. In this study, we applied organoids derived from induced pluripotent stem cells evaluate effect oxygen-glucose deprivation/reoxygenation (OGD/R). Pathway analysis showed relationships between vitamin digestion and absorption, fat peroxisome proliferator-activated receptor (PPAR) signaling pathway, complement coagulation...
Summary The brainstem controls heartbeat, blood pressure and respiration, which are life-sustaining functions, therefore, disorders of the can be lethal. Brain organoids derived from human pluripotent stem cells recapitulate course brain development expected to useful for medical research on central nervous system disorders. However, existing organoid models have limitations, hampering elucidation diseases affecting specific components brain. Here, we developed a method generate (hBSOs),...
Abstract Ischemic stroke is one of the most common neurological disease. However, impact ischemic on human cerebral tissue remains largely unknown; due to a lack brain samples. In this study, we used organoids derived from induced pluripotent stem cells evaluate effect oxygen-glucose deprivation/reoxygenation (OGD/R). We identified 15 differentially expressed genes (DEGs); and found that all DEGs were downregulated. Pathway analysis showed relationship vitamin digestion absorption, fat...