A5005582000- Genetics, Aging, and Longevity in Model Organisms
- RNA Research and Splicing
- DNA and Nucleic Acid Chemistry
- RNA and protein synthesis mechanisms
- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- X-ray Diffraction in Crystallography
- Spaceflight effects on biology
- Crystallization and Solubility Studies
- Crystallography and molecular interactions
- Cancer-related gene regulation
- Physiological and biochemical adaptations
- RNA modifications and cancer
- Nuclear Structure and Function
- Alzheimer's disease research and treatments
- Bacteriophages and microbial interactions
- Renal Diseases and Glomerulopathies
- Circadian rhythm and melatonin
- Pancreatic and Hepatic Oncology Research
- Chromosomal and Genetic Variations
- Inflammatory Myopathies and Dermatomyositis
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Inflammatory mediators and NSAID effects
National Institute of Advanced Industrial Science and Technology
2018-2024
Nippon Soken (Japan)
2019
RIKEN
2019
The Memory Clinic
2017
Sumitomo Dainippon Pharma (Japan)
2014
Kyushu University
2010-2011
Kyoto University
2001
Hiroshima University
1998
Showa University Fujigaoka Hospital
1988-1990
Guy's Hospital
1987
Animals facing conflicting sensory cues make a behavioral choice between competing alternatives through integration of the cues. Here, we performed genetic screen to identify genes important for two cues, attractive odorant diacetyl and aversive stimulus Cu 2+ , found that membrane-bound guanylyl cyclase GCY-28 receptor tyrosine kinase SCD-2 regulate these in Caenorhabditis elegans . The gcy-28 mutants scd-2 show an abnormal bias although their responses each individual cue are similar those...
Actin is a major structural component of the cytoskeleton in eukaryotic cells, and filamentous actin (F-actin) forms variety types cellular structures. Many probes have been developed to visualize F-actin architectures cells. However, it known that double-stained images obtained by two different often show partial inconsistencies. While developing probes, we observed merged three distinct each labeled with fluorescent dye (red, green blue), enabled various be distinguished based on color....
Liquid–liquid phase separation (LLPS) of proteins and DNA has recently emerged as a possible mechanism underlying the dynamic organization chromatin. We herein report role quadruplex folding in liquid droplet formation via LLPS induced by interactions between linker histone H1 (H1), key regulator chromatin organization. Fluidity measurements inside droplets, binding assays using G-quadruplex-selective probes, structural analyses based on circular dichroism demonstrated that structures, such...
Abstract Nuclear import receptors (NIRs) not only transport RNA-binding proteins (RBPs) but also modify phase transitions of RBPs by recognizing nuclear localization signals (NLSs). Toxic arginine-rich poly-dipeptides from C9orf72 interact with NIRs and cause nucleocytoplasmic deficit. However, the molecular basis for toxicity toward function as modifiers remains unidentified. Here we show that impede ability to RBPs. Isothermal titration calorimetry size-exclusion chromatography revealed...
A monoclonal antibody, designated TES101, was raised by immunizing BALB/c mice with an allogenic mouse testicular homogenate followed immunohistochemical selection as the initial screening method. By searching expressed sequence tag (EST) database N-terminal amino acid of TES101 reactive protein, we found that predicted encoded a EST clone matched protein sequence. Sequence analysis revealed no homologous molecule in DNA/protein database. Based on data obtained from derived contained signal...
Uncovering the sequence-encoded molecular grammar that governs liquid–liquid phase separation (LLPS) of proteins is a crucial issue to understand dynamic compartmentalization in living cells and emergence protocells. Here, we present model LLPS system induced by electrostatic interactions between anionic nucleic acids cationic oligolysine peptides modified with 12 different non-ionic amino acids, aim creating an index "phase-separation propensity" represents contribution LLPS. Based on...
Abstract The Drosophila behavior/human splicing protein family is involved in numerous steps of gene regulation. In humans, this consists three proteins: SFPQ, PSPC1, and NONO. Hemizygous loss-of-function (LoF) variants NONO cause a developmental delay with several complications (e.g., distinctive facial features, cardiac symptoms, skeletal symptoms) an X-linked recessive manner. Most the reported have been LoF variants, two missense as likely deleterious but no functional validation. We...
Liquid-liquid phase separation (LLPS) of proteins and DNAs has been recognized as a fundamental mechanism for the formation intracellular biomolecular condensates. Here, we show role constituent DNA components, i.e., phosphate groups, deoxyribose sugars, nucleobases, in LLPS with polycationic peptide, linker histone H1, known key regulator chromatin condensation. A comparison behavior mixtures H1 single-stranded DNA-based oligomers which one or more moieties were removed demonstrated that...
The dynamic properties of protein molecules are involved in the relationship between their structure and function. Time-resolved X-ray observation enables capturing structures biomolecules with picometre-scale precision. However, this technique has yet to be implemented living animals. Here, we examined diffracted blinking (DXB) tracking (DXT) observe dynamics a located on intestinal cells adult Caenorhabditis elegans. This vivo tissue-specific DXB was at temperatures from 20 °C -10 for...
IgG, IgA and IgM class antibodies to mouse laminin human fibronectin in sera from patients with various glomerular diseases (50 cases of nephropathy, 5 minimal-change nephrotic syndrome; 6 membranous systemic lupus erythematosus, 2 Henoch-Schönlein purpura, 3 poststreptococcal nephritis 4 preeclampsia) 30 normal controls were tested using a solid-phase enzyme-linked immunosorbent assay method. antimouse antibody titers nephropathy significantly higher (p < 0.05) than controls. There no...
Ultraviolet light is quite toxic to all the animals and evoke avoidance behavior of UV. The soil nematode Caenorhabditis elegans senses UV known avoid by using four sensory neurons. However, it not clear what signaling molecules act for in neuronal pathway constituted In addition, whether this harmful environmental signal can be associated with other benefit signals such as food. study, newly developed assay system, we found that C. associate food changes behavioral strategy against signal....
Post-translational modifications (PTMs) of histone proteins are recognized as critical factors governing the regulation chromatin condensation through liquid-liquid phase separation (LLPS). Here, we report impact acetylation sites within N-terminal H3 peptide on LLPS with nucleosomal linker DNA. In our model system mimicking partial structures, non-acetylated and DNA undergo driven primarily by electrostatic interactions. We found that enzymatic significantly inhibits LLPS, further...
Understanding the cellular environment as molecular crowding that supports structure-specific functional expression of biomolecules has recently attracted much attention. Time-resolved X-ray observations have remarkable capability to capture structural dynamics with subnanometre precision. Nevertheless, measurement intracellular within live organisms remains a challenge. Here, we explore potential utilizing crystallized proteins spontaneously form crystals investigate their via time-resolved...
Newborn neurons mature by distinct and sequential steps through the timely induction of specific gene expression programs in concert with epigenetic changes. However, it has been difficult to investigate relationship between changes at a single-cell resolution during neuronal maturation. In this study, we investigated maturation hermaphrodite-specific (HSNs) C. elegans, which provided link chromatin dynamics, expression, degree resolution. Our results demonstrated that composition promoter...
Liquid-liquid phase separation (LLPS) of proteins and DNA has recently emerged as a possible mechanism underlying the dynamic organization chromatin. We herein report role quadruplex folding in liquid droplet formation via LLPS induced by interactions between linker histone H1 (H1), key regulator chromatin organization. Fluidity measurements inside droplets binding assays using G-quadruplex-selective probes demonstrated that structures, such G-quadruplex i-motif, promote with decrease...
Accelerated anti-glomerular basement membrane nephritis was induced in rabbits, and the immunological, clinical histological evolution studied relation to urinary immunoreactive thromboxane B2 (i-TXB2) prostaglandin E2 (i-PGE2) excretion. In control nephritic animals, i-TXB2 increased 5-fold on day +1, but normal again by +5. The showed a positive correlation with creatinine clearance (CCr), proteinuria anti-sheep immunoglobuhn antibody. Urinary i-PGE2 excretion 50% indistinguishable later...
Abstract Dipeptide repeats (DPRs) that are gene products from abnormal hexanucleotide repeat expansion in C9orf72 trigger amyotrophic lateral sclerosis (ALS) through unknown mechanism. This study highlights, importin Karyopherinβ2 (Kapβ2), which is responsible for nuclear transport and phase modification of RNA-binding proteins (RBPs), as a major DPR target. We demonstrate accumulation the nucleus via Kapβ2-mediated transport, results dose-dependent toxicity observed nematode yeast models....
Post-translational modifications (PTMs) of histone proteins play a pivotal role in the regulation chromatin condensation, with evidence increasingly pointing to involvement liquid–liquid phase separation (LLPS). Here, we report significant impact acetylation site N-terminal H3 peptide on LLPS nucleosomal-linker DNA. In our model system, which mimics part structure chromatin, non-acetylated and DNA undergo LLPS, driven primarily by electrostatic interactions. Acetylation significantly...
Recently, advances in next-generation sequencing technologies have enabled genome-wide analyses of epigenetic modifications; however, it remains difficult to analyze the states histone modifications at a single-cell resolution living multicellular organisms because heterogeneity within cellular populations. Here we describe simple method visualize on specific sequence target locus Caenorhabditis elegans, by combining LacO/LacI system and genetically-encoded H4K20me1-specific probe,...
In most animals, avoiding pathogenic bacteria is crucial for better health and a long life span. For this purpose, animals should be able to quickly sense the presence or uptake of pathogens. The intestine could candidate organ induce escape behaviors; however, intestinal signaling mechanism acute regulation neuronal activity not well understood. Here, we show that adult Caenorhabditis elegans can respond bacterium Pseudomonas aeruginosa within 30 min exposure. This behavior was much faster...
Liquid-liquid phase separation (LLPS) of proteins and DNA has recently emerged as a possible mechanism underlying the dynamic organization chromatin. We herein report role quadruplex folding in liquid droplet formation via LLPS induced by interactions between linker histone H1 (H1), key regulator chromatin organization. <a>Fluidity measurements inside droplets, binding assays using G-quadruplex-selective probes, structural analyses based on circular dichroism demonstrated that...
Abstract ATPase family, AAA domain containing 2 (ATAD2), is a member of the bromodomain-containing protein family. ATAD2 associates with co-factors such as hormone receptors, E2Fs and c-Myc to control specific subset genes. Overexpression copy number alteration are observed in many types cancer, these alterations correlated poor clinical outcome breast, lung, endometrial cancers. Here, we present an integrated genomic analysis using Oncomine™ database that shows cancer-associated features...