Actin is a major structural component of the cytoskeleton in eukaryotic cells, and filamentous actin (F-actin) forms variety types cellular structures. Many probes have been developed to visualize F-actin architectures cells. However, it known that double-stained images obtained by two different often show partial inconsistencies. While developing probes, we observed merged three distinct each labeled with fluorescent dye (red, green blue), enabled various be distinguished based on color....

To treat malignant glioma, standard fractionated radiotherapy (RT; 60 Gy/30 fractions over 6 weeks) was performed post-surgery in combination with temozolomide to improve overall survival. Malignant glioblastoma recurrence rate is extremely high, and most recurrent tumors originate from the excision cavity high-dose irradiation region. In our previous study, protoporphyrin IX physicochemically enhanced reactive oxygen species generation by ionizing radiation combined treatment...

The genetic basis of sexual isolation that contributes to speciation is one the unsolved questions in evolutionary biology. Drosophila ananassae and pallidosa are closely related, postmating has not developed between them. However, females both species discriminate their mating partners, this discrimination strong By using surgical treatments, we demonstrate male courtship songs play a dominant role female mate discrimination. absence song D. dramatically increased interspecies with but...

Propolis, a resinous substance collected by honeybees mixing their saliva with plant sources, including tree bark and leaves then mixed secreted beeswax, possesses variety of bioactivities. Whereas caffeic acid phenethyl ester (CAPE) has been recognized as major bioactive ingredient in New Zealand propolis, Brazilian green on the other hand, artepillin C (ARC). In this study, we report that, similar to CAPE, ARC docks into abrogates mortalin-p53 complexes, causing activation p53 growth...

Abstract The intracellular accumulation of amyloid-β (Aβ) oligomers critically contributes to disease progression in Alzheimer’s (AD) and can be the potential target AD therapy. Direct observation molecular dynamics Aβ vivo is key for drug discovery research, however, it has been challenging because aggregation inhibits fluorescence from fusion proteins. Here, we developed 1-42 -GFP proteins that are oligomerized visualize their inside cells even when aggregated. We examined states Aβ-GFP...

Propylthiouracil (PTU) is known to cause vasculitis as a rare complication. We report the case of patient who developed alveolar haemorrhage and haematuria whilst treated with PTU. The serum was positive for antineutrophil cytoplasmic antibody (ANCA) myeloperoxidase (MPO) specificity (MPO-ANCA). All symptoms resolved completely after discontinuation Alveolar or pulmonary-renal syndrome associated may be new complication propylthiouracil therapy.

Due to the huge number of neuronal cells in brain and their complex circuit formation, computer simulation activity is indispensable understanding whole dynamics. Recently, various computational models have been developed based on whole-brain calcium imaging data. However, these analyses monitor only cell bodies treat as point unit. This point-neuron model inexpensive costs, but unrealistically simplistic at representing intact neural activities brain. Here, we describe a novel three-unit...

Drosophila ananassae and its relatives have many advantages as a model of genetic differentiation speciation. In this report, we examine evolutionary relationships in the species subgroup using multi-locus molecular data set, karyotypes, meiotic chromosome configuration, chromosomal inversions, morphological traits, patterns reproductive isolation. We describe several new taxa that are closest known D. ananassae. Analysis Y-chromosomal mitochondrial haplotypes, shared arrangements,...

Abstract The heterozygous deletion of 15q13.3 is a recurrently observed microdeletion syndrome associated with relatively mild phenotype including learning disability and language impairment. In contrast, the homozygous extremely rare much severer that includes epileptic encephalopathy, profound intellectual disability, hypotonia. Which genes within deleted interval responsible for more severe features when biallelically currently unknown. Here, we report patient hypotonia, seizures who had...

Colorectal cancer (CRC) is a heterogenous disease, and patients have differences in therapeutic response. However, the mechanisms underlying interpatient heterogeneity response to chemotherapeutic agents remain be elucidated, molecular tumor characteristics are required select for specific therapies. Patient-derived organoids (PDOs) established from CRCs recapitulate various biological of tissues, including cellular chemotherapy. show morphologies, but there no criteria defining these which...

Abstract. The courtship song emitted by male wing vibration has been regarded as one of the most important signals in sexual isolation species Drosophila melanogaster complex. Inter- and intraspecific crosses were observed using males whose wings removed (mute) or females aristae (deaf). Females D. melanogaster, simulans, mauritiana mated with heterospecific song-present condition (cross between normal winged males) more often than no-song wingless aristaless they showed no preference two...

A supramolecular nanohybrid based on carbon nanotubes and liposomes that is highly biocompatible capable of permeation through cells described. The can be loaded with a variety functional molecules structurally controlled by near-infrared laser irradiation for the release from nanohybrids in targeted manner via microscopy. We implemented demonstrated remote regulation photoinduced functions. As proof principle, amiloride were successfully used spatiotemporally blocking amiloride-sensitive...

Abstract Ice-binding proteins (IBPs) are capable of binding ice crystals and inhibiting their growth at freezing temperatures. IBPs also thought to stabilize the cell membrane non-freezing temperatures near 0 °C. These two effects have been assumed reduce cold- freezing-induced damage cells tissues. However, knowledge regarding IBP on living animals is limited. Here, we characterized relationship between physiological role by using nematode Caenorhabditis elegans . The expression fish...

Abstract Oligomers of intracellular amyloid β protein (Aβ) are strongly cytotoxic and play crucial roles in synaptic transmission cognitive function Alzheimer’s disease (AD). However, there is currently no AD model mouse which to specifically analyze the Aβ oligomers only. We have now developed a novel mouse, an Aβ-GFP transgenic (Aβ-GFP Tg), that expresses GFP-fused human 1-42 protein, forms only within neurons throughout their life. The fusion proteins expressed mainly hippocampal CA1-CA2...

Recent reports have suggested that 5-aminolevulinic acid (5-ALA), which is a precursor to protoporphyrin IX (PpIX), leads selective accumulation of PpIX in tumor cells and acts as radiation sensitizer vitro vivo mouse models melanoma, glioma, colon cancer. In this study, we investigated the effect under X-ray irradiation through ROS generation DNA damage. by interaction between was evaluated two kinds probes, 3′-(p-aminophenyl) fluorescein (APF) for hydroxyl radical (•OH) detection...

The neural networks that regulate animal behaviors are encoded in terms of neuronal excitation and inhibition at the synapse. However, how temporal activity circuits is dynamically precisely characterized by each signaling interaction via excitatory or inhibitory synapses, both synaptic patterns organized to achieve fine regulation circuit activities unclear. Here, we show Caenorhabditis elegans, excitatory/inhibitory switch from asymmetric sensory neurons (ASEL/R) following changes NaCl...

Sexual isolation is one of the most important mechanisms that may lead to speciation. Drosophila ananassae and D. pallidosa are useful for study sexual isolation, because their sympatric distribution no postmating between them. Courtship songs considered play a crucial role in pallidosa. We recorded analyzed male courtship eight four geographical strains, respectively. behaviors two species were consistent with those previously described, however, male's middle leg shaking, which had not...

Trypanosoma cruzi has three distinct life cycle stages; epimastigote, trypomastigote, and amastigote. Amastigote is the replication stage in host mammalian cells, hence this of parasite clinical significance drug development research. Presence extracellular amastigotes (EA) their infection capability have been known for some decades. Here, we demonstrate that EA can be utilized as an axenic culture to aid stage-specific study T. cruzi. Amastigote-like property amastigote sustained LIT medium...