A5045527843- Molecular Biology Techniques and Applications
- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- Monoclonal and Polyclonal Antibodies Research
- Electron Spin Resonance Studies
- Advanced Fiber Optic Sensors
- Identification and Quantification in Food
- Respiratory viral infections research
- Viral gastroenteritis research and epidemiology
- Coenzyme Q10 studies and effects
- Hepatitis C virus research
- Free Radicals and Antioxidants
- Viral Infections and Immunology Research
- Nanoparticles: synthesis and applications
- Analytical Chemistry and Sensors
- Food Quality and Safety Studies
- Virus-based gene therapy research
- Animal Disease Management and Epidemiology
- Influenza Virus Research Studies
- Biosensors and Analytical Detection
- Glycosylation and Glycoproteins Research
- RNA Research and Splicing
- Freezing and Crystallization Processes
- Vitamin C and Antioxidants Research
- Air Quality and Health Impacts
National Institute of Advanced Industrial Science and Technology
2008-2024
Gifu University
2017-2021
Yamagata University
2008
Shionogi (Japan)
1995
Proteins containing unnatural amino acids have immense potential in biotechnology and medicine. We prepared several histidine analogues including a novel analogue, β‐(1,2,3‐triazol‐4‐yl)‐dl‐alanine. These were assayed for translational activity histidine‐auxotrophic Escherichia coli strain UTH780. observed that analogues, our efficiently incorporated into the protein vivo; however, other rejected. results suggest hydrogen atom at specific position seriously affects incorporation.
Recent reports have suggested that 5-aminolevulinic acid (5-ALA), which is a precursor to protoporphyrin IX (PpIX), leads selective accumulation of PpIX in tumor cells and acts as radiation sensitizer vitro vivo mouse models melanoma, glioma, colon cancer. In this study, we investigated the effect under X-ray irradiation through ROS generation DNA damage. by interaction between was evaluated two kinds probes, 3′-(p-aminophenyl) fluorescein (APF) for hydroxyl radical (•OH) detection...
Highly sensitive viral analytical techniques are essential tools for preventing the spread of infections. In this study, we established a digital enzyme-linked immunosorbent assay (ELISA) system to quantify norovirus proteins with high sensitivity. We used norovirus-like particles (noro-VLPs) as surrogate and constructed two ELISA systems using different antibody pairs. The quantitative performance noro-VLP measurement each was evaluated. Both exhibited sensitivity, good linearity,...
The minus-IRES ((-)IRES), corresponding to the 3'-terminal end of negative strand hepatitis C virus (HCV) RNA, is well conserved among HCV subtypes. higher order structure (-)IRES essential for replication, because viral RNA dependent polymerase, NS5B, recognizes it as initiation site plus-strand synthesis genome. To inhibit "de novo" molecules, we performed an in vitro selection procedure aptamers that are specific domain I. Among selected aptamers, one aptamer had two binding sites We...
Abstract X-ray responsivity resulting in the generation of reactive oxygen species (ROS) was investigated 9600 organic compounds that were selected by considering their structural diversity. We focused on superoxides primarily detected using dihydroethidium (DHE) and hydroxyl radicals, identified fluorometrically 3’-(p-aminophenyl) Fluorescein (APF). Many discovered responded to DHE and/or APF assay irradiation. These results suggest some these emit either or radicals whereas others both...
The translation of HCV starts at the internal ribosomal entry site (IRES) within 5' untranslated region and IRES is well-conserved in strains. We developed a novel selection strategy using biotinylated oligonucleotide probe obtained RNA aptamers that bind domain II III-IV, respectively. Selected specifically bound to target sequence via RNA-RNA interactions. These inhibited IRES-depend vitro. Especially, 3-07 aptamer, which IIId, showed strong inhibition. Structure/function relationship...
本研究の目的は,制菌効果のある静水圧下,制菌効果のある炭酸ガス圧下において,小麦自身がもつ内在性酵素の活性を利用するドウの新規調製法の可能性を見いだすことである.静水圧処理は50 MPa 30℃,炭酸ガス圧処理は1.0 40℃で制菌効果を確認することができた.本加圧条件下において,小麦粉内在性酵素による澱粉の分解作用によると考えられる糖の増加が確認できたのに加えて,呈味性遊離アミノ酸として知られるグルタミン酸の増加が確認できた.これらのことから良好な甘みと香ばしい外皮,そしてイーストの活性促進というメリットが期待できる.さらに,加圧処理状態においてドウに人為的にアミラーゼなどの酵素を添加することで,さらなる糖の増加や,グルテン分解によるアレルギー対策に寄与するといった活用法の開発が期待される.
The hybrid method upon combining rolling circle amplification and loop-mediated isothermal (RCA-LAMP) was developed to quantify very small amount of different type RNAs, such as miRNAs. RCA-LAMP can help detect short sequences through padlock probe (PLP) circularization exhibit powerful DNA amplification. However, one the factors that determines detection limit is non-specific In this study, we improved accuracy applying RNase H-dependent PCR (rhPCR) technology. method, suppressed by using...
Highly sensitive viral analytical techniques are essential tools for preventing the spread of infections. In this study, we established a digital enzyme-linked immunosorbent assay (ELISA) system to quantify norovirus proteins...
The translation of HCV starts at the internal ribosomal entry site (IRES) within 5' untranslated region and domain II IRES is essential for activity. However, information function limited. We attempted to obtain RNA that bind II. Selected aptamers will give us much information, such as RNA-RNA interaction between other cellular RNAs well target sites anti-HCV drugs. developed a novel selection method using biotinylated DNA probe. This can be applied many would identify most suitable position...
AbstractWe measured Asc.− (ascorbate radical) produced by the reaction of AscH− (ascorbic acid) with HO. or O.−2 after irradiation in mouse. It is possible to measure easily using ESR and a dialysis method which was collected at room temperature interstitial fluid through membrane. After irradiation, increased both normal muscle tumour tissues (SCC-VII) proportion radiation dose. treatment agents H2O2 FeCl2, while it decreased SOD catalase. These results suggest that amount reflected...
In this paper, we propose a new evaluation method using external standard RNA for quality control of the extracted RNA. Integrity Number and UV absorption are generally used as basis control; however, these methods do not always reflect mRNA. While is supposedly designed on mRNA, it has potential to be evaluate study, took into consideration three essential factors, viz., yield inhibition DNA polymerase, degradation mRNA determining It would possible know enzyme reaction by adding before...
Ribonucleic acid (RNA) quantification is an essential technique in biology. There has been remarkable progress RNA techniques over the recent years; however, specificity of these to quantify a very small amount doubtful because factors which can inhibit precise quantification. To develop that leads most sensitive quantification, problems must be overcome. In this article, we first review RNA: quality RNA, secondary structure efficiency enzyme reaction, annealing conditions, limitations...