A5100667522- Veterinary Oncology Research
- Fluorine in Organic Chemistry
- Infectious Diseases and Mycology
- Synthesis and Biological Evaluation
- Synthesis and Reactions of Organic Compounds
- RNA Interference and Gene Delivery
- Cyclopropane Reaction Mechanisms
- Sarcoma Diagnosis and Treatment
- MicroRNA in disease regulation
- Advanced NMR Techniques and Applications
- Advanced MRI Techniques and Applications
- Synthetic Organic Chemistry Methods
- Virus-based gene therapy research
- Synthesis and Catalytic Reactions
- Oral and Maxillofacial Pathology
- Bone Tumor Diagnosis and Treatments
- Electron Spin Resonance Studies
- Salivary Gland Tumors Diagnosis and Treatment
- Immunotherapy and Immune Responses
- Synthesis and Reactivity of Heterocycles
- Chemical Reactions and Isotopes
- Gastrointestinal Tumor Research and Treatment
- Cancer-related molecular mechanisms research
- Wnt/β-catenin signaling in development and cancer
- Asymmetric Synthesis and Catalysis
Gifu University
2016-2025
Nara Institute of Science and Technology
2018
The University of Tokyo
1993-2015
Yamaguchi University
2015
Kobe University
2014-2015
Wakayama Medical University
2009-2011
Sapporo Medical University
2011
University of Tsukuba
2008
Bunkyo University
2003-2007
Tohoku University
2002-2006
MicroRNAs regulate gene expression by repressing translation or directing sequence-specific degradation of their complementary mRNA. We recently reported that miR-203 is down-regulated, and its exogenous inhibits cell growth in canine oral malignant melanoma tissue specimens as well human cells. A microRNA target database predicted E2F3 ZBP-89 putative targets microRNA-203 (miR-203). The levels E2F3a, E2F3b, were markedly up-regulated Mewo cells compared with those epidermal melanocytes....
Abstract In human and dogs, bladder cancer ( BC ) is the most common neoplasm affecting urinary tract. Dog resembles muscle‐invasive in histopathological characteristics gene expression profiles, could be an important research model for this disease. Cancer patient‐derived organoid culture can recapitulate organ structures maintains profiles of original tumor tissues. a previous study, we generated dog prostate organoids using urine samples, however had never been produced. Therefore aimed...
A novel agent, (R)-(-)-2-propyloctanoic acid (ONO-2506), has a unique property in that it modulates functions of activated cultured astrocytes, including pronounced inhibition S-100beta synthesis. The present study examined whether administration this agent would mitigate the delayed expansion infarct volume and neurologic deficits after permanent middle cerebral artery occlusion (pMCAO) rats. Daily intravenous ONO-2506 (10 mg/kg) abolished between 24 168 hours pMCAO, whereas acute until was...
Bladder cancer (BC), a main neoplasm of urinary tract, is usually inoperable and unresponsive to chemotherapy. As novel experimental model for muscle-invasive BC, we previously established culture method dog BC organoids. In the present study, detailed in vitro vivo anti‐tumor effects trametinib were investigated by using this model. each organoid strain, epidermal growth factor receptor (EGFR)/ERK signaling was upregulated compared with normal bladder cells. Trametinib even at low...
An astrocytic protein S-100β enhances the expression of inducible nitric oxide synthase in cultured astrocytes at micromolar concentrations, leading to oxide-mediated death cocultured neurons. The present study examined whether production by reactive accumulating within periinfarct area was related delayed expansion infarct volume after permanent middle cerebral artery occlusion rat. After rapid increases during initial 24 hours, increase then decelerated while maintaining increasing...
MicroRNA-145 (miRNA-145; miR-145) is aberrantly expressed in most of human cancers and plays a significant role carcinogenesis cancer progression. In the current study, we focused on how miR-145 canine malignant melanomas. MiR-145 was significantly downregulated melanoma tissues cell lines, as well lines tested. The ectopic expression showed growth inhibition both cells tested, effect achieved partly by suppressing c-MYC LMeC A2058 Mewo cells. At same time, suppressive tendency migration...
Colorectal cancer is one of the leading causes cancer-related death worldwide. Previous studies have shown that miR-92a has an oncogenic function in several cancers and its up-regulation correlated with malignant clinicopathologic behaviors colorectal cancer. It also been suggested circulating patients' plasma can be a potential biomarker for However, precise roles intracellular extracellular are not yet understood. In this study, we examined expression levels tumors (38 specimens 56 adenoma...
Dog spontaneously develop prostate cancer ( PC ) like humans. Because most dogs with have a poor prognosis, they could be used as translational model for advanced in Stem cell‐derived 3‐D organoid culture recapitulate organ structures and physiology. Using patient tissues, human system was established. Recent study has shown that urine cells also possess the characteristic of stem cells. However, organoids never been produced. Therefore, we generated using dog samples. Urine were...
Circulating microRNAs in the blood may provide diagnostic and prognostic information about canine neoplastic diseases, their profiles be conserved between human species. We performed RT-qPCR to obtain of circulating plasma microRNA-214 -126 total 181 cases diseases healthy controls. MicroRNA-214 levels were high 2 epithelial tumours (thyroid mammary carcinomas) 4 non-epithelial (osteosarcoma, histiocytic sarcoma, chondrosarcoma, hemangiosarcoma). In contrast, microRNA-126 6 (mammary,...
Abstract Three-dimensional (3D) organoid culture holds great promises in cancer precision medicine. However, Matrigel and stem cell-stimulating supplements are necessary for culturing 3D cells. It costs a lot of money consumes more time effort compared with 2D cultured Therefore, the establishment cheaper Matrigel-free that can maintain characteristics part organoids is demanded. In previous study, we established dog bladder (BC) system by using their urine samples. Here, successfully...
Dog bladder cancer (BC) is mostly muscle-invasive (MI) with poor prognosis, and its pathogenesis close to human MIBC. Three-dimensional (3D) organoid culture ensures novel knowledge on diseases including BC. Recently, we have established dog BC organoids (BCO) using their urine samples. BCO recapitulated the epithelial structures, characteristics, drug sensitivity of BC-diseased dogs. However, from normal epithelium are not yet. Therefore, present study aimed establish (NBO) for further...
[reaction: see text] A ruthenium catalyst precursor bearing a bulky and electron-donating pentamethylcyclopentadienyl (Cp) ligand, typically CpRuH3(PPh3), mediates hydrosilylation of several 1-alkynes with novel regioselectivity to give preferentially 2-silyl-1-alkenes.
In order to analyze the detailed mechanisms responsible for macrophage activation by chitin derivatives, resident peritoneal macrophages were prepared and stimulated with chitin, chitosan low-molecular weight chitosan. Our findings as follows: (i) induced apoptosis of macrophages, but this did not occur when or water soluble used; (ii) treatment markers, such major histocompatibility complex (MHC) class I, II, Fc receptors, transferrin receptor, mannose Fas, inflammatory protein (MIP)-2,...
microRNA (miR)-205 is downregulated and acts as a tumor suppressor in human melanoma cells. Previously, for clinical application, we added aromatic benzene-pyridine (BP-type) analogs to the 3′-overhang region of RNA-strand changed sequences passenger strand miR-143 duplex. Here, demonstrated antitumor effect vitro vivo miR-205 that was also chemically modified by BP had altered sequence. In experiments, transfection with synthetic (miR-205BP/S3) significantly inhibited growth Exogenous...
Malignant melanoma (MM) is one of the most aggressive cancers in dogs and humans. However, molecular mechanisms its development progression remain unclear. Presently, we examined expression profile microRNAs (miRs) canine oral MM tissues paired normal mucosa by using microRNA-microarray assay quantitative RT-PCR. Importantly, a decreased miR-203 was significantly associated with shorter survival time. Also, -205 were markedly down-regulated human cell lines tested. Furthermore, ectopic...
Although intracranial and spinal cord meningioma prognoses have been reported, few studies evaluated the outcomes of orbital optic nerve meningiomas in dogs. We aimed to evaluate canine meningiomas. The seven dogs included were cytologically or histopathologically diagnosed with Four underwent first-line surgery; three received postoperative radiation therapy. remaining therapy; however, 372 days after therapy, one dog surgery as lesion progressed size. Five surgical resection, had...
A new monoclonal antibody (MAb), 22-1-1, acts against a novel tumor-associated antigen (Ag) strongly expressed in human uterine cervical adenocarcinomas. cDNA encoding the Ag recognized by 22-1-1 MAb has been isolated and called RCAS1 (receptor-binding cancer on SiSo cells). can induce growth arrest apoptosis receptor-positive cells including T natural killer vitro. These results suggest that is involved tumor escape from immune system. Immunohistochemical analysis revealed relationship...
Disfavored 5-endo-trig cyclizations were achieved in 2-trifluoromethyl-1-alkenes with a nucleophilic nitrogen, oxygen, sulfur, or carbon atom through 1) intramolecular S(N)2' reaction loss of fluoride ion 2) addition to the vinylic group. This manifold provides versatile method for synthesis indolines, indoles, pyrrolidines, tetrahydrofurans, 2,3-dihydrobenzo[b]thiophenes, tetrahydrothiophenes, and cyclopentanes that bear fluorinated one-carbon unit such as difluoromethylene, difluoromethyl,...