A5048646076- Neuroscience and Neuropharmacology Research
- Adenosine and Purinergic Signaling
- Retinal Development and Disorders
- Wnt/β-catenin signaling in development and cancer
- Neuroscience of respiration and sleep
- Alzheimer's disease research and treatments
- Neurogenesis and neuroplasticity mechanisms
- Pain Mechanisms and Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Cellular Mechanics and Interactions
- Cancer-related gene regulation
- Prion Diseases and Protein Misfolding
- Sleep and Wakefulness Research
- Neurological diseases and metabolism
- Parkinson's Disease Mechanisms and Treatments
- Pharmacological Receptor Mechanisms and Effects
- Biochemical effects in animals
- Vestibular and auditory disorders
- Skin and Cellular Biology Research
- Ion channel regulation and function
- Retinoids in leukemia and cellular processes
- Luminescence Properties of Advanced Materials
- Receptor Mechanisms and Signaling
- Axon Guidance and Neuronal Signaling
- Nuclear Receptors and Signaling
National Institute of Advanced Industrial Science and Technology
1998-2021
University of Gothenburg
2010
Brigham and Women's Hospital
2002-2003
National Academy of Medicine
2003
Harvard University
2003
Harvard University Press
2002
Fuchu Hospital
1993-1998
University of Tsukuba
1989-1994
Tokyo Metropolitan Institute of Medical Science
1993
Actin is a major structural component of the cytoskeleton in eukaryotic cells, and filamentous actin (F-actin) forms variety types cellular structures. Many probes have been developed to visualize F-actin architectures cells. However, it known that double-stained images obtained by two different often show partial inconsistencies. While developing probes, we observed merged three distinct each labeled with fluorescent dye (red, green blue), enabled various be distinguished based on color....
Monoclonal antibodies were generated against the adenosine A1 receptor (A1R) purified from rat brain. In immunoblot analyses of or partially A1R preparations brain, these recognized a solitary band, size which corresponded to that expected for A1R. These not only native form but also deglycosylated Immunocytochemical analysis Chinese hamster ovarian cells transfected stably with cDNA showed their cell bodies stained intensely by antibodies, whereas nontransfected not. detected naturally...
δ-Catenin is a neuronal protein that contains 10 Armadillo motifs and binds to the juxtamembrane segment of classical cadherins. We report δ-catenin interacts with cortactin in tyrosine phosphorylation–dependent manner. This interaction occurs within region sequence also essential for neurite elongation effects. Src family kinases can phosphorylate bind through its polyproline tract. Under conditions when phosphorylation reduced, cells extend unbranched primary processes. Conversely,...
Abstract Transcriptional profiling (TP) offers a powerful approach to identify genes activated during memory formation and, by inference, the molecular pathways involved. Trace eyeblink conditioning is well suited for study of regional gene expression because it requires hippocampus, whereas highly parallel task, delay conditioning, does not. First, we determined when was most regulated trace conditioning. Rats were exposed 200 trials per day paired and unpaired stimuli each 4 days. Changes...
Delta-catenin is a synaptic adherens junction protein pivotally positioned to serve as signaling sensor and integrator. Expression of delta-catenin induces filopodia-like protrusions in neurons. Here we show that the small GTPases Rho family act coordinately downstream effectors delta-catenin. A dominant negative Rac prevented delta-catenin-induced protrusions, Cdc42 activity was dramatically increased by expression. kinase dead LIMK (LIM kinase) mutant Cofilin also protrusions. To link...
Abstract The intracellular accumulation of amyloid-β (Aβ) oligomers critically contributes to disease progression in Alzheimer’s (AD) and can be the potential target AD therapy. Direct observation molecular dynamics Aβ vivo is key for drug discovery research, however, it has been challenging because aggregation inhibits fluorescence from fusion proteins. Here, we developed 1-42 -GFP proteins that are oligomerized visualize their inside cells even when aggregated. We examined states Aβ-GFP...
Cranial radiotherapy in children often leads to progressive cognitive decline. We have established a rodent model of irradiation-induced injury the young brain. A single dose 8 Gy was administered left hemisphere postnatal day 10 (P10) mice. Harlequin (Hq) mice, carrying hypomorphic apoptosis-inducing factor AIFHq mutation, express 60% less AIF at P10 and displayed significantly fewer dying cells subventricular zone (SVZ) 6 h after IR, compared with wild type (Wt) littermates. Irradiated...
1. Activity of inspiratory neurons was recorded extracellularly from the caudal portion nucleus ambiguous (0-3.5 mm rostral to obex) in decerebrated, spontaneously breathing cats. Using a micropressure ejection method, we tested responsiveness direct applications serotonin (5-HT) and noradrenaline (NA) comparison with glutamate control artificial cerebrospinal fluid (ACSF) by means multibarreled micropipette. 2. We made detailed examinations 52 that were excited but did not react ACSF. Those...
Calcium/calmodulin-dependent protein kinase II is a prominent enzyme in the mammalian brain that phosphorylates variety of substrate proteins. In present study, monoclonal antibodies specifically recognize either alpha or beta isoforms this were used to determine distribution these within rat and monkey spinal cord. rat, corticospinal tract consists two components: dorsal tract, which occupies ventralmost aspect funiculus; ventral an area adjacent median fissure. Both fibers strongly...
Abstract Oligomers of intracellular amyloid β protein (Aβ) are strongly cytotoxic and play crucial roles in synaptic transmission cognitive function Alzheimer’s disease (AD). However, there is currently no AD model mouse which to specifically analyze the Aβ oligomers only. We have now developed a novel mouse, an Aβ-GFP transgenic (Aβ-GFP Tg), that expresses GFP-fused human 1-42 protein, forms only within neurons throughout their life. The fusion proteins expressed mainly hippocampal CA1-CA2...
Adenosine has an anticonvulsant effect in various models of epilepsy. This appears to be mediated through the activation adenosine A1 receptors (A1Rs). We immunohistochemically investigated changes A1Rs expression kainate-treated and hippocampus-kindled rats as chronic In normal hippocampus, a predominant was detected CA2/CA3a field. The immunoreactivity this field began decline drastically approximately 4 weeks after kainate treatment remained minimal 8 treatment. animals, stimulated side...
Intracellular amyloid-β (Aβ) oligomers are key therapeutic targets because they strongly cytotoxic and play crucial roles in the cognitive function Alzheimer's disease (AD). Anthocyanins, polyphenolic flavonoids with antioxidant neuroprotective properties, potential candidates for AD. Here, we investigated effects of anthocyanin-enriched extracts from fruits mulberry (Morus alba Linn.) Thailand against neurotoxicity Aβ oligomers. Using monitoring system aggregation, showed that extract...