A5045015921- Epigenetics and DNA Methylation
- DNA Repair Mechanisms
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- Computational Drug Discovery Methods
- 14-3-3 protein interactions
- Microtubule and mitosis dynamics
- Ubiquitin and proteasome pathways
- Monoclonal and Polyclonal Antibodies Research
- Peptidase Inhibition and Analysis
- Cancer-related Molecular Pathways
- MicroRNA in disease regulation
- Bioinformatics and Genomic Networks
- Histone Deacetylase Inhibitors Research
- Cancer Mechanisms and Therapy
- Viral Infectious Diseases and Gene Expression in Insects
- HER2/EGFR in Cancer Research
- DNA and Nucleic Acid Chemistry
- Bacterial Genetics and Biotechnology
- Melanoma and MAPK Pathways
- Carcinogens and Genotoxicity Assessment
- Cancer-related gene regulation
- Acute Myeloid Leukemia Research
- Synthesis and biological activity
- Photosynthetic Processes and Mechanisms
Advanced Centre for Treatment, Research and Education in Cancer
2016-2025
Tata Memorial Hospital
2016-2025
Amity University
2024
Homi Bhabha National Institute
2023
Indian Institute of Information Technology Allahabad
2018
Cancer Research Institute
2015
Indian Institute of Science Bangalore
2011
Histones are abundant nuclear proteins that essential for the packaging of eukaryotic DNA into chromosomes. Different histone variants, in combination with their modification 'code', control regulation gene expression diverse cellular processes. Several enzymes catalyze addition and removal multiple modifications have been discovered past decade, enabling investigations role(s) normal processes pathological conditions. This sudden influx data, however, has resulted need an updated...
Epigenetics research is progressing in basic, pre-clinical and clinical studies using various model systems. Hence, updating the knowledge integration of biological data emerging from silico, vitro vivo for different epigenetic factors essential. Moreover, new drugs are being discovered which target proteins, tested studies, trials approved by FDA. It brings distinct challenges as well opportunities to update existing HIstome database implementing applying enormous biomedical...
Recent global health concern motivated the exploration of natural medicinal plant resources as an alternative target for treating COVID-19 infection and associated inflammation. In current study, a phytochemical, 6-shogaol [1-(4-hydroxy-3-methoxyphenyl)dec-4-en-3-one; 6-SHO] was investigated potential anti-inflammatory anti-COVID-19 agent. virus release assay, 6-SHO efficiently (94.5%) inhibited SARS-CoV2 replication. When tested in inflammasome activation model, displayed mechanistic action...
Why cancer cells disproportionately accumulate polyubiquitinated proteotoxic proteins despite high proteasomal activity is an outstanding question. While mis-regulated ubiquitination a contributing factor, here we show that structurally-perturbed and sub-optimally functioning proteasome at the core of altered proteostasis in tumors. By integrating gene coexpression signatures subunits breast (BrCa) patient tissues with atomistic details 26S holocomplex, find transcriptional deregulation...
Glycogen synthase kinase-3 (GSK-3) has been known to regulate various cellular and metabolic processes, including neuronal plasticity. Dysregulation of the GSK-3β isoform leads development neurological disorders such as Alzheimer’s, Parkinson’s, Huntington’s disease. Thus, gained major attention for therapeutic intervention in disorders. The present study aimed explore natural derivatives value potential inhibitors consideration treatment Using an In silico approach through virtual...
Mutations in the TP53 gene may lead to loss of its tumor suppressor function and acquisition oncogenic properties. The enhanced stability mutant p53 (mutp53) is one pivotal factors for functions, rendering proteins implicated mutp53 stabilization as promising targets therapeutic intervention. Although deubiquitinases (DUBs) are commonly deregulated various cancers, their specific impact on remains largely unexplored. In this study, we demonstrated involvement DUBs—USP5 USP9X in—enhancing...
<title>Abstract</title> Many HER2-positive breast cancer (BC) patients relapse within a year of trastuzumab or neratinib treatment. We identified specific pathogenic mutations in the dimerization domains II and IV HER2 receptor that contribute to treatment resistance. Mutations G309A, S310Y, P523S induce significant structural alterations, disrupting crucial HER2:HER2 binding pockets. HER3-preferring mutants exhibited increased HER2:HER3 interactions, as confirmed by proximity ligation assay...
HtrA2, a trimeric proapoptotic serine protease is involved in several diseases including cancer and neurodegenerative disorders. Its unique ability to mediate apoptosis via multiple pathways makes it an important therapeutic target. In C-terminal PDZ domain upon substrate binding regulates its functions through coordinated conformational changes the mechanism of which yet be elucidated. Although allostery has been found some homologs, not characterized HtrA2 so far. Here, with silico...
The oral cancer gene database has been compiled to enable fast retrieval of updated information and role the genes implicated in cancer.The first version with 242 was published Online Journal Bioinformatics 8(1), 41-44, 2007.In second version, enlarged include 374 by adding 132 entries.The architecture format is similar earlier includes external hyperlinks for all genes.The functional interaction network important biological processes molecular functions rebuilt based on using 'String...
Genome-wide expression profiling studies have identified four core molecular subgroups of medulloblastoma: WNT, SHH, Group 3 and 4. Molecular markers are necessary for accurate risk stratification in the non-WNT due to underlying heterogeneity genetic alterations overall survival. MiR-204 was evaluated molecularly classified 260 medulloblastomas from an Indian cohort 763 MAGIC cohort, SickKids, Canada. Low miR-204 / 4 tumors identify a highly aggressive subset having poor survival, two...
The maintenance of DNA sequence integrity is critical to avoid accumulation cancer-causing mutations. Inactivation Mismatch Repair (MMR) genes (e.g., MLH1 and MSH2) common among many cancers, including colorectal cancer (CRC) the driver classic microsatellite instability (MSI) in tumors. Somatic MSH3 alterations have been linked a specific form MSI called elevated at selected tetranucleotide repeats (EMAST) that associated with patient poor prognosis African American (AA) rectal patients....
The distinct functional effects of the replication-dependent histone H2A isoforms have been demonstrated; however, mechanistic basis non-redundancy remains unclear. Here, we investigated specific contribution isoform H2A1H, which differs from another H2A2A3 in identity only three amino acids. H2A1H exhibits varied expression levels different normal tissues and human cancer cell lines (H2A1C humans). It also promotes proliferation a context-dependent manner when exogenously overexpressed. To...
Abstract Tobacco‐related oral cancer is the most common among Indian males, gingivo‐buccal complex (GBC) being affected subsite due to habit of chewing tobacco. Proteins from lysates microdissected normal and transformed epithelium clinically well‐characterized tissue samples GBC were separated by two‐dimensional gel electrophoresis identify differentially expressed proteins. Eleven protein spots showed differential expression, which could withstand stringency statistical evaluation. The...
Deinococcus RecA (DrRecA) protein is a key repair enzyme and contributes to efficient DNA of radiodurans. Phosphorylation DrRecA at Y77 (tyrosine 77) T318 (threonine 318) residues modifies the structural conformational switching that impart efficiency activity DrRecA. Dynamics comparisons with its phosphorylated analogues support idea phosphorylation sites could change dynamics conformation plasticity Furthermore, docking studies showed increases binding preference towards dATP versus ATP...
BRCA1 associated ring domain protein 1(BARD1) is a tumor suppressor having wide role in cellular processes like cell-cycle checkpoint, DNA damage repair and maintenance of genomic integrity. Germ-line mutation Gln 564 His discovered linker region BARD1 leads to loss binding Cleavage stimulating factor (CstF50), which turn instigates the premature mRNA transcript formation apoptosis. We have studied dynamics ARD present wild-type mutant association with CstF50 using biophysical, biochemical...
Abstract Medulloblastoma, a common pediatric malignant brain tumor, consists of four distinct molecular subgroups WNT, SHH, Group 3 and 4. Exome sequencing 11 WNT subgroup medulloblastomas from an Indian cohort identified mutations in several chromatin modifier genes, including genes the mammalian SWI/SNF complex. The genome tumors is known to be stable except for monosomy 6. Two tumors, having 6, carried loss function mutation ARID1B gene located on chromosome expression also lower compared...
Histone modifications occur in precise patterns, with several known to affect the binding of proteins. These interactions chromatin structure, gene regulation, and cell cycle events. The dual on H3 tail, serine10 phosphorylation, lysine14 acetylation (H3Ser10PLys14Ac) are reported be crucial for interaction 14-3-3ζ. However, mechanism by which H3Ser10P along neighboring site-specific acetylation(s) is targeted its regulatory proteins, including kinase phosphatase, not fully understood. We...
Acute myeloid leukemia (AML) with NPM1 mutation is a disease driving genetic alteration good prognosis. Although it has been suggested that induces chemosensitivity in leukemic cells, the underlying cause for better survival of mutated patients still not clear. Mutant AML unique microRNA and their target gene (mRNA) signature compared to wild-type . Dynamic regulation miRNA‐mRNA reported influence prognostic outcome. In present study, silico expression data miRNA mRNA was retrieved from...