A5053101130- Protein Kinase Regulation and GTPase Signaling
- Cellular transport and secretion
- Muscle Physiology and Disorders
- PI3K/AKT/mTOR signaling in cancer
- Cell death mechanisms and regulation
- Cardiomyopathy and Myosin Studies
- Microtubule and mitosis dynamics
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Nuclear Structure and Function
- RNA and protein synthesis mechanisms
- Pancreatic function and diabetes
- Proteoglycans and glycosaminoglycans research
- HIV Research and Treatment
- Genomics and Phylogenetic Studies
- Cellular Mechanics and Interactions
- Neurogenetic and Muscular Disorders Research
- Osteoarthritis Treatment and Mechanisms
- Genomics and Rare Diseases
- Skin and Cellular Biology Research
- Metabolism, Diabetes, and Cancer
- Cancer-related gene regulation
- Cell Adhesion Molecules Research
- Chromosomal and Genetic Variations
- Fungal and yeast genetics research
- DNA and Nucleic Acid Chemistry
Institut Jacques Monod
2002-2019
Université Paris Cité
1980-2019
Centre National de la Recherche Scientifique
1996-2013
Sorbonne Paris Cité
2013
Délégation Paris 7
2012
European Molecular Biology Laboratory
2002
Laboratoire de Microbiologie et Génétique Moléculaires
1996-1999
Inserm
1984-1994
Krev-1 is known to suppress transformation by ras. However, the mechanism of suppression unclear. The protein product Krev-1, Rap1A-p21, identical Ras-p21 proteins in region where interaction with guanosine triphosphatase (GTPase) activating (GAP) believed occur. Therefore, ability GAP interact Rap1A-p21 was tested. not activated but bound tightly and an effective competitive inhibitor GAP-mediated Ras-GTPase activity. Binding strictly triphosphate (GTP)-dependent. bind may account for This...
ABSTRACT Among the small GTPases of Ras family, Rap proteins exhibit highest homology with p21Ras. The four so far identified constitute two subgroups, comprising Rap1(A,B) and Rap2(A,B) proteins. intracellular location Rap1A, Rap1B Rap2A was investigated in mammalian cells by confocal immunofluorescence microscopy. Using a specific anti-Rap1 affinity-purified antibody, both Rap1A were localized to late endocytic compartments (late endosomes/lysosomes) fibroblasts. localization B transiently...
Journal Article Nucleotide sequence of a human cDNA encoding ras-related protein (rap1B) Get access V. Pizon, Pizon INSERM U-248, Faculté de Médecine LariboisiÈreSaint-Louis 10, avenue verdun, 75010 Paris, France Search for other works by this author on: Oxford Academic PubMed Google Scholar I. Lerosey, Lerosey P. Chardin, Chardin A. Tavitian Nucleic Acids Research, Volume 16, Issue 15, 11 August 1988, Page 7719, https://doi.org/10.1093/nar/16.15.7719 Published: 1988 history Accepted: 08 July
Assembly of muscle sarcomeres is a complex dynamic process and involves large number proteins. A growing these have regulatory functions are transiently present in the myofibril. We show here that novel tubulin-associated RING/B-box protein MURF2 associates with microtubules, myosin titin during sarcomere assembly. During assembly, first microtubules at exclusion tyrosinated tubulin. Then, MURF2-labelled associate sarcomeric later A-band when non-striated myofibrils differentiate into mature...
How a given Ras prreotein coordinates multiple signaling inputs and outputs is fundamental issue of specificity. Schizosaccharomyces pombe contains one Ras, Ras1, that has two distinct outputs. Ras1 activates Scd1, presumptive guanine nucleotide exchange factor (GEF) for Cdc42, to control morphogenesis chromosome segregation, Byr2, component mitogen-activated protein kinase cascade, mating. So far there only established GEF, Ste6. Paradoxically, ste6 null (ste6Δ) mutants are sterile but...
We have investigated the sequence organization of very small genome (DNA content/haploid cell c = 0.4-0.5 pg) a tetraodontid fish, Arotron diadematus, by using two main experimental approaches. The first one, renaturation kinetics, showed that slowly reassociating, intermediate, fast and foldback sequences represented 87%, 7%, 5% 1%, respectively, A. diadematus DNA, which is, so far, vertebrate DNA lowest in repeated sequences. second approach, centrifugation Cs2SO4/BAMD density gradients...
The ubiquitin proteasome system and macroautophagy are proteolytic pathways essential in the maintenance of cellular homeostasis during differentiation remodelling skeletal muscle. In both pathways, proteins to be degraded tagged with polyubiquitin. muscles, MURF2 display E3 ligase structure suggesting that they may covalently attach polypeptides still unknown target proteins. So far only MURF2A isoforms were studied shown interact p62/SQSTM1, a protein implicated macroautophagic...
Three human cDNAs encoding new RAS-related cDNAs, designated RAP1A, RAP1B, and RAP2, have been isolated previously. The encoded proteins are highly related to RAS in the effector region share an overall identity with of approximately 50%. Using complete or parts thereof as probes, each RAP gene has localized on chromosomes by situ hybridization. three genes RAP2 assigned chromosome bands 1p12----p13, 12q14, 13q34, respectively.
Abstract Ras oncogenes encode 21‐kDa (p21s) GTP binding proteins that are capable of transforming immortalized cells in culture. The ras ‐related rap 1A/Krev‐1/smgp21A protein, exhibits a similar structural organization and contains the same effector domain as proteins, antagonizes ‐transformation. In order to investigate whether closely related (61% identical) rap2 protein had capacities, corresponding cDNA was inserted into constitutive well inducible mammalian expression vectors. Neither...
The centrosome regulates cell spatial organisation by controlling the architecture of microtubule (MT) cytoskeleton. Conversely, position within depends on cytoskeletal networks it helps organizing. In mammalian cells, positioning involves a population MT stably anchored at centrioles, core components centrosome. An MT-anchoring complex containing proteins ninein and Cep170 is enriched subdistal appendages (SAP) that decorate older centriole (called mother centriole) proximal ends. Here, we...
Mechanics is now recognized as crucial in cell function. To date, the mechanical properties of cells have been inferred from experiments which investigate roles actin and microtubules ignoring intermediate filaments (IFs) contribution. Here, we analyse myoblasts behaviour context myofibrillar myopathy resulting p.D399Y desmin mutation disorganizes IF network muscle cells. We compare response expressing either mutated or wild-type to cyclic stretch. Cells are cultivated on supports submitted...
Similarity between the carboxyl‐terminal portion of human epidermal growth factor (EGF) receptor and deduced protein sequence chicken‐derived oncogene v‐ erbB , avian erythroblastosis virus strain H, has suggested that chicken cellular locus, c‐ might be part a longer EGF‐receptor gene in chicken, whose entire coding capacity remained to defined. The locus spans more than 20 × 10 3 base pairs (20 kbp) DNA contains at least 1.8 kbp homologous oncogene. We show here EGF cDNA genomic share...
The GTPase activity of membranes isolated from differentiated HL‐60 cells was investigated to obtain information about the possible involvement membrane‐bound GTP‐binding proteins in regulation NADPH oxidase. A more than tenfold increase rate hydrolysis GTP observed when cytosol and arachidonic acid were added simultanously, i.e. under same conditions where oxidase becomes activated. There parallel changes activities concentration or species fatty varied different detergents applied....