Abstract As genomic analysis technology has advanced, it become possible to sub-classify intrahepatic cholangiocarcinoma (ICC) at the histological or molecular level. Here, we verify recently suggested two subgroups of ICC in organoids model, compare characteristics between types. patients are subclassified into small-duct (SD) and large-duct (LD) subtype according characteristics. established, unsupervised principal component clustering separates each type ICC. Differential gene expression...

Background/Aim: Natural killer (NK) cells are one of the lymphocytes clinically used for various cancer types. Cytotoxicity NK to cholangiocarcinoma (CC), however, has not yet been studied. Nor cell therapy against CC applied. In this study, relevance anti-tumor efficacy was pre-clinically investigated. Materials and Methods: Human HuCCT-1 cells, an intrahepatic line, were xenografted into nude mice. The tumor-bearing mice then received multiple infusions ex vivo-expanded human (SMT01) in...

Cholangiocarcinoma is a devastating malignancy with fatal complications that exhibits low response and resistance to chemotherapy. Here, we evaluated the anticancer effects of CG200745, novel histone deacetylase inhibitor, either alone or in combination standard chemotherapy drugs cholangiocarcinoma cells. CG200745 dose-dependently reduced viability cells vitro decreased tumour volume weight xenograft model. Administering along other chemotherapeutic agents including gemcitabine,...

Objective Gait analysis plays a pivotal role in evaluating walking abilities, with recent advancements digital health stressing the importance of efficient data collection methods. This study aims to classify nine gait types including one normal and eight abnormal gaits, using sequential network-based models diverse feature combinations obtained from insole sensors. Methods The dataset was collected sensors subjects performing 15 m designated types. incorporated pressure inertial measurement...

The identification of novel tumor-specific proteins or antigens is great importance for diagnostic and therapeutic applications in pancreatic cancer. Using oligonucleotide microarrays, we identified a broad spectrum differentially expressed cancer-related genes. Of these, selected an overexpressed sequence taq cloned 721-bp full-length cDNA with open reading frame 196 amino acids. This gene was localized on the Homo sapiens 16p13.3 chromosomal locus, its nucleotide matched similar to common...

Hedgehog (Hh) signaling is frequently up-regulated in fibrogenic pancreatic diseases including chronic pancreatitis and cancer. Although recent series suggest exclusive paracrine activation of stromal cells by Hh ligands from epithelial components, debates still exist on how works pathologic conditions. To explore affects the pancreas, we investigated transgenic phenotypes zebrafish that over-express either Indian or Sonic along with green fluorescence protein (GFP) to enable real-time...

Carbohydrate antigen (CA) 19-9 is the only diagnostic marker used in pancreatic cancer despite its limitations. Here, we aimed to identify role of CEMIP (also called KIAA1199) combined with CA patients cancer. A retrospective analysis prospectively collected patient samples was performed determine benefit markers diagnosis We investigated and levels 324 49 normal controls using serum enzyme-linked immunosorbent assay. Median were 410.5 U/ml (40.8-3342.5) 0.67 ng/ml (0.40-1.08), respectively,...

Ongoing characterization of glioma has revealed that Akt signaling plays a crucial role in gliomagenesis. In mouse models, however, alone was not sufficient to induce glioma. We established transgenic zebrafish overexpressed dominant-active (DA) human Akt1 or Rac1G12V (DARac1) at ptf1a domain and investigated phenotypes mechanisms leading Transgene expressions were spatiotemporally restricted without any developmental abnormality embryos persisted cerebellum medulla adult zebrafish. DAAkt1...

The expression of leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 2 (LINGO2) has been reported in Parkinson’s disease; however, its role other diseases is unknown. Gastric cancer the second leading cause death. Cancer stem cells (CSC) are a subpopulation that contribute to initiation invasion cancer. We identified LINGO2 as CSC-associated gastric cancers both vitro patient-derived tissues. studied effect on cell motility, stemness,...

<abstract> <p>Early detection of the risk sarcopenia at younger ages is crucial for implementing preventive strategies, fostering healthy muscle development, and minimizing negative impact on health aging. In this study, we propose a novel technique that combines surface electromyography (sEMG) signals empirical mode decomposition (EMD) with machine learning algorithms. First, recorded preprocessed sEMG data from both at-risk individuals during various physical activities,...

Abstract Background Biliary tract cancer (BTC) is a relatively rare but aggressive gastrointestinal with high mortality rate. Cancer stem cell (CSC) populations play crucial roles in tumor biology and are responsible for the low response to anti-cancer treatment recurrence This study investigated role of Transgelin-2 ( TAGLN2 ), overexpressed CSC BTC cells, analyzed its expression patient tissues serum identify potential new targets BTC. Methods was suppressed by small-interfering or short...

Typically, tokenization is the very first step in most text processing works. As a token serves as an atomic unit that embeds contextual information of text, how to define plays decisive role performance model. Even though Byte Pair Encoding (BPE) has been considered de facto standard method due its simplicity and universality, it still remains unclear whether BPE works best across all languages tasks. In this paper, we test several strategies order answer our primary research question, is,...

Cognitive impairment in the elderly causes a significant decline quality of life and substantial economic burden on society. Yet, diagnosing cognitive is apt to be missed or delayed due its assessment being laborious. This study aimed propose new approach classifying risk using sequential gait characteristics machine learning techniques. A total 108 community-dwelling individuals participated this study. The participants were categorized into three groups based their scores mini-mental state...

// Do Yeon Lee 1 , Dawoon E. Jung 2 Sung Sook Yu 3 Yeo Song 4 Beom Ku Choi 5 and Yong Chan Department of Internal Medicine, Yonsei University College Seoul, Korea Institute Gastroenterology, Biomedical Science, Samsung Medical Research Center, Immune & Cell Therapy Branch, Division Cancer Biology, National Gyeonggi-do, Correspondence to: Lee, email: leeyc@yuhs.ac Keywords: helicobacter pylori CagA, SIRT3, HIF-1α, gastric cancer Received: December 22, 2016 Accepted: May 2017 Published: June...

Abstract Pancreatic cancer remains one of the most common and lethal cancers. Most patients (80%) present with inoperable advanced pancreatic at initial diagnosis, their early diagnosis is a significant unmet challenge. Recent studies indicate that cancer, including initiated propagated by stem cells (CSCs). CSCs are responsible not only for pathogenesis but also heterogeneity, malignant degree, anticancer therapy resistance, recurrence tumors. Therefore, identification may be crucial...

We propose a wearable sensor-based gait classification system. Our approach assumes that multiple IMU sensors attached to various body parts can capture the characteristics are used predict whether subject has foot abnormalities or athletic performance. first transform raw sensor signals into spectrogram images and feed this visual representation deep CNN models. The data were acquired from 7 pelvis, thighs, shanks, feet while 69 people in three groups walked on 20-m straight path....

This phase I/II study evaluated the safety and efficacy of a new histone deacetylase (HDAC) inhibitor, ivaltinostat, in combination with gemcitabine erlotinib for advanced pancreatic ductal adenocarcinoma (PDAC). Patients diagnosed unresectable, histologically confirmed PDAC who had not undergone previous therapy were eligible. Phase I 3 + dose escalation design to determine maximum tolerable (MTD) ivaltinostat (intravenously on days 1, 8 15) (1000 mg/m2 intravenously (100 mg/day, orally)...

Embigin is a member of the immunoglobulin superfamily and encodes transmembrane glycoprotein. There have been reports involvement in neuromuscular junction formation plasticity; however, molecular functions other organs are unknown. Our aim was to investigate possible role pancreatic cancer. In ductal adenocarcinoma tissues, expression higher than that normal tissues. Immunohistochemical analysis revealed cancer cells, as well monocarboxylate transporter 2 (MCT2) To gain further insight, we...

Faced with the rapidly aging world population, frailty has emerged as a major health burden among elderly. This study aimed to investigate feasibility of using temporal gait characteristics and long short-term memory network for assessing frailty. Seventy-four community-dwelling elderly individuals participated in this study. The participants were categorized into three groups by their FRAIL scale: robust, pre-frail, frail groups. completed 7-meter walking at self-selected pace gyroscope on...

Severe combined immunodeficiency (SCID) mice have widely been used as hosts for human tumor cell xenograft study. This animal model, however, is labor intensive. As zebrafish largely emerging a promising model system studying diseases including cancer, developing efficient immunocompromised strains study are also demanded in zebrafish. Here, we created the Prkdc-null SCID which provides stable immune-deficient background required xenotransplantation of cell. In this study, two transcription...

Human gait has served as a useful barometer of health. Existing studies for automatic categorization have been limited to binary classification pathological and non-pathological provided low accuracy in multi-classification. This study aimed propose novel approach that can multi-classify with no visually discernible difference characteristics. Sixty-nine participants without disturbance were recruited. Twenty-nine the semi-professional athletes, 19 ordinary people. The remaining 21 people...

Objective To examine the cardiorespiratory responses of patients with spinal cord injury (SCI) paraplegia using a motor driven rowing machine. Method Ten SCI [A (n=6), B (n=1), and C (n=3) by American Spinal Injury Association impairment scale] were selected. Two techniques used. The first used fixed seat achieved only upper extremity movement (fixed rowing). second an automatically moving seat, facilitating active passive lower via motorized (motor Each patient performed two randomly...

Human gait has been regarded as a useful behavioral biometric trait for personal identification and authentication. This study aimed to propose an effective approach classifying gait, measured using wearable inertial sensors, based on neural networks. The 3-axis accelerometer gyroscope data were acquired at the posterior pelvis, both thighs, shanks, feet while 29 semi-professional athletes, 19 participants with normal foot, 21 patients foot deformities walked 20-meter straight path....