A5103257445- Immunotherapy and Immune Responses
- Epigenetics and DNA Methylation
- Infrastructure Maintenance and Monitoring
- Zebrafish Biomedical Research Applications
- Kruppel-like factors research
- Advanced Breast Cancer Therapies
- Reliability and Maintenance Optimization
- T-cell and B-cell Immunology
- Meta-analysis and systematic reviews
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Cytokine Signaling Pathways and Interactions
- Facilities and Workplace Management
- Phagocytosis and Immune Regulation
- Ethics in Clinical Research
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Pancreatic and Hepatic Oncology Research
- Cancer Genomics and Diagnostics
- Hedgehog Signaling Pathway Studies
- Artificial Intelligence in Healthcare and Education
- Lung Cancer Research Studies
- Cancer Immunotherapy and Biomarkers
- Erythropoietin and Anemia Treatment
- Management of metastatic bone disease
- Extracellular vesicles in disease
Yonsei University
2015-2025
University of Auckland
2024
CHA Vaccine Institute (South Korea)
2023-2024
Severance Hospital
2013-2020
University Health System
2020
Yonsei University Health System
2020
Core Research Center
2013
Korea Institute of Brain Science
2013
Norwegian Womens Public Health Association
2013
Korea University
2013
Background/Aim: Natural killer (NK) cells are one of the lymphocytes clinically used for various cancer types. Cytotoxicity NK to cholangiocarcinoma (CC), however, has not yet been studied. Nor cell therapy against CC applied. In this study, relevance anti-tumor efficacy was pre-clinically investigated. Materials and Methods: Human HuCCT-1 cells, an intrahepatic line, were xenografted into nude mice. The tumor-bearing mice then received multiple infusions ex vivo-expanded human (SMT01) in...
Chronic inflammation is an important process leading to tumorigenesis. Therefore, targeting and controlling can be a promising cancer therapy. Inflammation often caused by variety of inflammatory cytokine such as the interleukin (IL)-6, pleiotrophic known involved in In this study, vivo hepatic tumorigenesis model zebrafish was generated demonstrate direct consequence human IL6 expression causing hepatocarcinogenesis. To do this, elevated hIL6 gene established specifically target hepatocytes...
Hedgehog (Hh) signaling is frequently up-regulated in fibrogenic pancreatic diseases including chronic pancreatitis and cancer. Although recent series suggest exclusive paracrine activation of stromal cells by Hh ligands from epithelial components, debates still exist on how works pathologic conditions. To explore affects the pancreas, we investigated transgenic phenotypes zebrafish that over-express either Indian or Sonic along with green fluorescence protein (GFP) to enable real-time...
We present an application of systematic probing for selecting optimal maintenance, repair, and reconstruction (MR&R) policies systems infrastructure facilities under model uncertainty. use open-loop feedback control approach, where the parameters are updated sequentially after every inspection round. The improves convergence by ensuring that all permissible actions applied to condition state. results parametric analyses demonstrate MR&R converge earlier when is used. However, savings in...
Ongoing characterization of glioma has revealed that Akt signaling plays a crucial role in gliomagenesis. In mouse models, however, alone was not sufficient to induce glioma. We established transgenic zebrafish overexpressed dominant-active (DA) human Akt1 or Rac1G12V (DARac1) at ptf1a domain and investigated phenotypes mechanisms leading Transgene expressions were spatiotemporally restricted without any developmental abnormality embryos persisted cerebellum medulla adult zebrafish. DAAkt1...
Sirt1, a member of the sirtuin gene family, encodes most conserved mammalian NAD+-dependent deacetylase enzyme responsible for removing acetyl groups from many proteins. The Sirt1 is known as longevity whose knockout in mice leads to decreased lifespan relative wild type. This study aimed explore phenotypic changes zebrafish Sirt1-knockouts and investigate function gene. Targeted (Danio rerio) was achieved using CRISPR-Cas9 genome editing system. We created 4-bp insertion-homozygote...
Severe combined immunodeficiency (SCID) mice have widely been used as hosts for human tumor cell xenograft study. This animal model, however, is labor intensive. As zebrafish largely emerging a promising model system studying diseases including cancer, developing efficient immunocompromised strains study are also demanded in zebrafish. Here, we created the Prkdc-null SCID which provides stable immune-deficient background required xenotransplantation of cell. In this study, two transcription...
TLR agonists have emerged as an efficient cancer vaccine adjuvant system that induces robust immune responses. L-pampo™, a proprietary of TLR2 and TLR3 agonists, promotes strong humoral cellular responses against infectious diseases. In this study, we demonstrate vaccines formulated with L-pampo™ affect the recruitment activation dendritic cells (DCs) in draining lymph nodes (dLNs) leading to antigen-specific T-cell anti-tumor efficacy. We analyzed DC maturation proliferation using flow...
Iroquois homeobox 1 (IRX1) belongs to the family known play an important role during embryonic development. Interestingly, however, recent studies have suggested that IRX1 also acts as a tumor suppressor. Here, we use homozygous knockout mutants of zebrafish demonstrate gene is true suppressor and mechanism suppression mediated by repressing cell cycle progression. In this study, found Irx1 induced hyperplasia tumorigenesis in multiple organs where was expressed. On other hands,...
Dendritic cells (DCs) are key antigen-presenting that prime naive T and initiate adaptive immunity. Although the genetic deficiency transgenic overexpression of granulocyte macrophage-colony stimulating factor (GM-CSF) signaling were reported to influence homeostasis DCs, in vivo development DC subsets following injection GM-CSF has not been analyzed detail. Among treatment mice with different hematopoietic cytokines, only generates a distinct subset XCR1 - 33D1 DCs which make up majority...
Frequent neutropenia hinders uninterrupted palbociclib treatment in patients with hormone receptor (HR)-positive breast cancer. We compared the efficacy outcomes multicenter cohorts of metastatic cancer (mBC) receiving following conventional dose modification or limited modified schemes for afebrile grade 3 neutropenia.Patients HR-positive, human epidermal growth factor 2-negative mBC (n=434) letrozole as first-line therapy were analyzed and classified based on management follows: group 1...
Dendritic cells (DCs) in peripheral tissues may have a unique role to regulate innate and adaptive immune responses antigens that enter the tissues. Peritoneal cavity is body compartment surrounding various organs housing diverse cells. Here, we investigated specialized features of classical DC (cDC) subsets following intraperitoneal injection model antigen ovalbumin (OVA). cDC1s were superior cDC2s activating OVA-specific CD8 T cells, while both cDCs similar stimulating CD4 Each peritoneal...
Abstract Introduction: Immunotherapy is a promising therapeutic approach for wide range of cancers. However, some tumors called "cold tumors" lack infiltrating T-cells and are not responsive to immune checkpoint inhibitors (ICIs), leading worse clinical outcomes. This study demonstrates that L-pampo, novel TLR2/3 agonist, promotes an anti-tumor response in cold tumors. Also, combining L-pampo with ICIs can improve the efficacy more safely than standard-of-care (SoC) chemotherapies. Method:...
Abstract Introduction: Despite advances in anticancer treatments, there has been urgent need to identify novel prognostic markers for HER2-negative AGC. We propose AI based deep learning and machine pipeline explore genetic that predict patient response chemotherapy Methods: retrospectively assessed 179 stage IV AGC patients who have treated with first-line Yonsei Cancer Center, Korea between 2015 2021. In-house targeted sequencing panel data (CancerSCANTM CancerMaster) were used candidate...
Allergic asthma was typically considered as an inflammatory disease mediated by type 2 immunity. However, recent studies revealed that is a complex displaying variety of phenotypes and endotypes.We examined cellular in the mouse model allergic sensitized with different adjuvants. The aim our study to determine immunologic characteristics models induced ovalbumin (OVA) adjuvants.Mice were intraperitoneally admixture OVA various adjuvants such Alhydrogel (alum), papain, lipopolysaccharide...
441 Background: Circulating tumor DNA (ctDNA) is a minimally invasive approach, effective for the real-time follow-up of cancer. However, clinical significance in gastric cancer not yet determined. Therefore, we investigated both ctDNA and tissue biopsy NGS at diagnosis stage IV patients to evaluate relationship with parameters concordance NGS. Methods: All samples were sequenced targeted panel sequencing – CancerMaster Panel V2 (in-house 522 gene panel, Kwon et al., ASCO GI 2021) or...