A5073656126- Bacterial Infections and Vaccines
- Amoebic Infections and Treatments
- Vector-borne infectious diseases
- Reproductive tract infections research
- Mosquito-borne diseases and control
- Antimicrobial Peptides and Activities
- Vibrio bacteria research studies
- Cell Adhesion Molecules Research
- Viral Infections and Vectors
- Neonatal and Maternal Infections
- Complement system in diseases
- Toxin Mechanisms and Immunotoxins
- Clostridium difficile and Clostridium perfringens research
- Immune Response and Inflammation
- PARP inhibition in cancer therapy
- interferon and immune responses
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Influenza Virus Research Studies
- Calcium signaling and nucleotide metabolism
- Neuroinflammation and Neurodegeneration Mechanisms
- Tryptophan and brain disorders
- Toxoplasma gondii Research Studies
- Aquaculture disease management and microbiota
- Hepatitis B Virus Studies
Boston Children's Hospital
2021-2024
University of Virginia
2012-2024
Harvard University
2021-2024
Boston Children's Museum
2022
Virginia Commonwealth University
2011-2012
Abstract CR3 (CD11b/CD18; αmβ2 integrin) is a conserved phagocytic receptor. The active conformation of binds the iC3b fragment complement C3 as well many host and microbial ligands, leading to actin-dependent phagocytosis. There are conflicting reports about how engagement affects fate phagocytosed substrates. Using imaging flow cytometry, we confirmed that binding internalization iC3b-opsonized polystyrene beads by primary human neutrophils was CR3-dependent. did not stimulate neutrophil...
ABSTRACT Anaplasma phagocytophilum is the tick-transmitted obligate intracellular bacterium that causes human granulocytic anaplasmosis (HGA). A. binding to sialyl Lewis x (sLe ) and other sialylated glycans decorate P selectin glycoprotein 1 (PSGL-1) glycoproteins critical for infection of mammalian host cells. Here, we demonstrate importance outer membrane protein A (OmpA) APH_0338 in OmpA transcriptionally induced during transmission feeding -infected ticks on mice upregulated invasion...
Symptomatic infection by Neisseria gonorrhoeae (Gc) produces a potent inflammatory response, resulting in neutrophil-rich exudate. A population of Gc can survive the killing activities neutrophils for reasons not completely understood. Unlike other Gram-negative bacteria, releases monomeric peptidoglycan (PG) extracellularly, dependent on two nonessential, nonredundant lytic transglycosylases (LTs), LtgA and LtgD. PG released LtgD stimulate host immune responses. We report that ΔltgAΔltgD...
The mucosal inflammatory response to Neisseria gonorrhoeae (Gc) is characterized by recruitment of neutrophils the site infection. Gc survives exposure limiting ability make antimicrobial products and expressing factors that defend against these products. multiple transferable resistance (Mtr) system a tripartite efflux pump, comprised inner membrane MtrD, periplasmic attachment protein MtrC, outer channel MtrE. MtrCDE exports diverse array substrates, including certain detergents, dyes,...
ABSTRACT Anaplasma phagocytophilum , a member of the family Anaplasmataceae is tick-transmitted obligate intracellular bacterium that causes human granulocytic anaplasmosis. The life cycle A. biphasic, transitioning between noninfectious reticulate cell (RC) and infectious dense-cored (DC) forms. We analyzed bacterium's DC surface proteome by selective biotinylation proteins, NeutrAvidin affinity purification, mass spectrometry. Transcriptional profiling selected outer membrane protein...
Anaplasma phagocytophilum is an obligate intracellular bacterium that invades neutrophils to cause the emerging infectious disease human granulocytic anaplasmosis. A. undergoes a biphasic developmental cycle, transitioning between dense-cored cell (DC) and noninfectious reticulate (RC). To gain insights into organism's biology pathogenesis during myeloid infection, we conducted proteomic analyses on organisms purified from HL-60 cells. A total of 324 proteins were unambiguously identified,...
Neisseria gonorrhoeae (the gonococcus, Gc) causes the sexually transmitted infection gonorrhea. Gc is a prominent threat to human health by causing severe lifelong sequelae, including infertility and chronic pelvic pain, which amplified emergence of “superbug” strains resistant all current antibiotics. highly adapted colonize mucosal surfaces, where it survives despite initiating robust inflammatory response influx polymorphonuclear leukocytes (PMNs, neutrophils) that typically clear...
The bacterial pathogen Neisseria gonorrhoeae (Gc) infects mucosal sites rich in antimicrobial proteins, including the cell wall-degrading enzyme lysozyme. Certain Gram-negative bacteria produce protein inhibitors that bind to and inhibit Here, we identify Ng_1063 as a new inhibitor of lysozyme Gc, define its functions light second, recently identified inhibitor, Ng_1981. In silico analyses indicated bears sequence structural homology MliC-type Recombinant inhibited lysozyme-mediated killing...
An emerging theme among vacuole-adapted bacterial pathogens is the ability to hijack ubiquitin machinery modulate host cellular processes and secure pathogen survival. Mono- polyubiquitination differentially dictate subcellular localization, activity, fate of protein substrates. Monoubiquitination directs membrane traffic from plasma endosome has been shown promote autophagy. Anaplasma phagocytophilum an obligate intracellular bacterium that replicates within a cell-derived vacuole co-opts...
The Gram-negative pathogen Neisseria gonorrhoeae (gonococcus [Gc]) colonizes lysozyme-rich mucosal surfaces. Lysozyme hydrolyzes peptidoglycan, leading to bacterial lysis. Gc expresses two proteins, SliC and NgACP, that bind inhibit the enzymatic activity of lysozyme. is a surface-exposed lipoprotein, while NgACP found in periplasm also released extracellularly. Purified similarly However, whereas mutation ngACP increases susceptibility lysozyme, sliC mutant only susceptible lysozyme when...
ABSTRACT Neisseria gonorrhoeae (the gonococcus, Gc) is the causative agent of sexually transmitted infection gonorrhea. Gc a prominent threat to human health by causing severe and lifelong clinical sequelae, including infertility chronic pelvic pain, which amplified emergence “superbug” strains that are resistant all current antibiotics. highly adapted colonize mucosal surfaces, where it survives despite initiating robust inflammatory response influx polymorphonuclear leukocytes (PMNs or...
Single-stranded DNA containing CGT/A motifs binds to the helicase domain of Schlafen 11 (SLFN11) initiate cell death and cytokine production via SLFN11 ribonuclease activity (see related Research Article by Zhang et al. ).
ABSTRACT Complement receptor 3 (CR3; CD11b/CD18; α m β 2 integrin) is a conserved phagocytic receptor. The active conformation of CR3 binds the iC3b fragment complement C3 as well many host and microbial ligands, leading to actin-dependent phagocytosis. There are conflicting reports about how engagement affects fate phagocytosed substrates. Using imaging flow cytometry, we confirmed that binding internalization iC3b-opsonized polystyrene beads by primary human neutrophils was CR3-dependent....
Central to health and disease are innate immune receptors that bind bacterial molecules initiate inflammation host defense. Beyond pathogens their membrane disruptive activities, mechanisms enabling access in the cytoplasm unknown. Here, we describe cytoplasmic cyclic dinucleotide (CDN)-binding protein STING as a common sensor. Irrespective of virulence after bacteriolysis phagolysosomes, CDNs produced during infections with evolutionarily diverse bacteria activate STING. Of several known...