A5000402254- Bacterial Infections and Vaccines
- Tuberculosis Research and Epidemiology
- Complement system in diseases
- Reproductive tract infections research
- Autophagy in Disease and Therapy
- Monoclonal and Polyclonal Antibodies Research
- Cell Adhesion Molecules Research
- Mycobacterium research and diagnosis
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Animal Nutrition and Physiology
- Immune cells in cancer
- Phagocytosis and Immune Regulation
- Glycosylation and Glycoproteins Research
- Probiotics and Fermented Foods
- Herbal Medicine Research Studies
- RNA and protein synthesis mechanisms
- interferon and immune responses
- Sarcoma Diagnosis and Treatment
- Blood disorders and treatments
- Autoimmune and Inflammatory Disorders Research
- Cytokine Signaling Pathways and Interactions
- Antifungal resistance and susceptibility
- Peptidase Inhibition and Analysis
- Sarcoidosis and Beryllium Toxicity Research
- Biochemical Acid Research Studies
University of Virginia
2013-2023
Washington University in St. Louis
2022-2023
Institute for Women's Policy Research
2023
Luhansk State Medical University
2018
New York State College of Veterinary Medicine
2010
Cornell University
2010
Kaplan Medical Center
1999-2007
Hebrew University of Jerusalem
2003-2006
Schneider Children's Medical Center
2001
Mucin glycoproteins play a key role in the regular function of epithelium gastrointestinal tract, and this study, ontogenesis development mucin producing cells was examined broiler. Mucin-producing were observed small intestine from 3 d before hatch, at time contained only acidic mucin. After hatch until Day 7 posthatch, proximal, middle, distal segments similar proportions goblet neutral mucins. A gradient cell density increasing along duodenal to ileal axis. Delayed access feed for 48 h...
The protective mucus layer covers the entire surface of gastrointestinal tract. also acts as a medium for molecule transport between luminal contents and enterocytes; therefore it has major role in nutrient absorption. main component, mucin glycoproteins, is produced by mucous-secreting goblet cells. In chicken small intestine, functional development cells enterocytes occurs late embryonic immediate posthatch period. Presence crucial mucosal development. Feed deprivation immediately after...
The immune response to Mycobacterium tuberculosis infection determines disease outcomes, yet we have an incomplete understanding of what factors contribute a protective response. Neutrophilic inflammation has been associated with poor prognosis in humans and animal models during M. and, therefore, must be tightly regulated. ATG5 is essential autophagy protein that required innate cells control neutrophil-dominated promote survival infection; however, the mechanistic basis for how regulates...
1. In order to determine the feasibility of using high fibre diets in turkey rations, three crude dietary concentrations were fed hens at ages and performance, digestibility small intestinal morphology determined. 2. Growth rate feed efficiency decreased when contained 80 90 g fibre/kg; however, growth did not change 60 fibre/kg was between 1 4 weeks or 6 8 enhanced 11 14 age. 3. Digestibilities protein, fat gross energy (GE) depressed intakes g/kg but later ages. Crude increased with age...
Chlamydiae replicate within a nonacidified vacuole, termed an inclusion. As obligate intracellular bacteria, chlamydiae actively modify their vacuole to exploit host signaling and trafficking pathways. Recently, we demonstrated that several Rab GTPases are targeted the To define biological roles of inclusion localized GTPases, have begun identify inclusion-localized effectors. Here demonstrate oculocerebrorenal syndrome Lowe protein 1 (OCRL1), Golgi complex-localized phosphatidylinositol...
Abstract CR3 (CD11b/CD18; αmβ2 integrin) is a conserved phagocytic receptor. The active conformation of binds the iC3b fragment complement C3 as well many host and microbial ligands, leading to actin-dependent phagocytosis. There are conflicting reports about how engagement affects fate phagocytosed substrates. Using imaging flow cytometry, we confirmed that binding internalization iC3b-opsonized polystyrene beads by primary human neutrophils was CR3-dependent. did not stimulate neutrophil...
Neisseria gonorrhoeae (Gc) is a human-specific pathogen that causes the sexually transmitted infection gonorrhea. Gc survives in neutrophil-rich gonorrheal secretions, and recovered bacteria predominantly express phase-variable, surface-expressed opacity-associated (Opa) proteins (Opa+). However, expression of Opa like OpaD decreases survival when exposed to human neutrophils ex vivo. Here, we made unexpected observation incubation with normal serum, which found inflamed mucosal enhances...
ABSTRACT Neisseria gonorrhoeae (the gonococcus, Gc) triggers a potent inflammatory response and recruitment of neutrophils to the site infection. Gc survives exposure despite these cells' antimicrobial products, such as reactive oxygen species (ROS). ROS production in is initiated by NADPH oxidase, which converts into superoxide. The subunits oxidase are spatially separated between granules (gp91 phox /p22 ) cytoplasm (p47 , p67 p40 ). Activation promotes coassembly at phagosome and/or...
Neutrophils are the most abundant cell type in airways of tuberculosis patients. Mycobacterium (Mtb) infection induces release neutrophil extracellular traps (NETs); however, molecular regulation and impact NET on Mtb pathogenesis unknown. We find that during neutrophils, PAD4 citrullinates histones to decondense chromatin gets released as NETs a manner can maintain viability promote replication. Type I interferon promotes formation chromatin-containing vesicles allow without compromising...
ATG5: autophagy related 5; ATG7: 7; ATG14: 14; ATG16L1: 16-like 1 (S. cerevisiae); BECN1: beclin 1, related; CASP1: caspase 1; CASP4/CASP11: 4, apoptosis-related cysteine peptidase; CIM: conditionally immortalized macrophage; CLP: cecal ligation and puncture; CSS: cytokine storm syndrome; DC: dendritic cell; IFNG/IFNγ: interferon gamma; IFNGR1: gamma receptor ip: intraperitoneal; iv: intravenous; IL12/p70: interleukin 12, p70 heterodimer; IL18: Interleukin 18; ITGAX/CD11c: integrin alpha X;...
Abstract Quantifying the efficiency of particle uptake by host cells is important in fields infectious diseases, autoimmunity, cancer, developmental biology, and drug delivery. Here we present a protocol for high‐throughput analysis imaging flow cytometry, using bacterium Neisseria gonorrhoeae attached to internalized neutrophils as an example. Cells are exposed fluorescently labeled bacteria, fixed, stained with bacteria‐specific antibody different fluorophore. Thus, absence permeabilizing...
The rise in multidrug resistant tuberculosis cases underscores the urgent need to develop new treatment strategies for tuberculosis. Herein, we report discovery and synthesis of a series compounds containing 3-thio-1,2,4-triazole moiety that show inhibition Mycobacterium (Mtb) growth survival. Structure–activity relationship studies led us identify several potent analogs displaying low micromolar nanomolar inhibitory activity, specifically against Mtb. demonstrated no cytotoxicity mammalian...
Polymorphisms in the
Abstract Better understanding of the host responses to Mycobacterium tuberculosis infections is required prevent and develop new therapeutic interventions. The transcription factor BHLHE40 essential for controlling M. infection, in part by repressing Il10 expression, where excess IL-10 contributes early susceptibility Bhlhe40−/− mice infection. Deletion Bhlhe40 lung macrophages dendritic cells sufficient increase but how impacts macrophage cell unknown. In this study, we report that myeloid...
Polymorphisms in the IRGM gene are associated with susceptibility to tuberculosis humans. A murine ortholog of Irgm, Irgm1, is also essential for controlling Mycobacterium (Mtb) infection mice. Multiple processes have been IRGM1 activity that could impact host response Mtb infection, including roles autophagy-mediated pathogen clearance and expansion activated T cells. However, what IRGM1-mediated pathway necessary control vivo mechanistic basis this remains unknown. We dissected...
Abstract Human carcinoembryonic antigen‐related cell adhesion molecules (CEACAMs) are a family of receptors that mediate intercellular interactions. Pathogenic bacteria have ligands bind CEACAMs on human cells. Neisseria gonorrhoeae (Gc) encodes numerous unique outer membrane opacity‐associated (Opa) proteins for one or more CEACAMs. expressed epithelial cells facilitate Gc colonization, while those neutrophils affect phagocytosis and consequent intracellular survival Gc. Since Opa protein...