A5070534269- Bacterial Infections and Vaccines
- Complement system in diseases
- Reproductive tract infections research
- Cell Adhesion Molecules Research
- Pneumonia and Respiratory Infections
- Monoclonal and Polyclonal Antibodies Research
- Advancements in Transdermal Drug Delivery
- Immune cells in cancer
- Thermal Regulation in Medicine
- Nanoparticles: synthesis and applications
- Renal Diseases and Glomerulopathies
- Peptidase Inhibition and Analysis
- Cardiac Arrest and Resuscitation
- Immune Response and Inflammation
- Nanoparticle-Based Drug Delivery
- Virology and Viral Diseases
- Immunotherapy and Immune Responses
- Blood groups and transfusion
- Respiratory Support and Mechanisms
Walter Reed Army Institute of Research
2024
University of Virginia
2020-2023
Abstract CR3 (CD11b/CD18; αmβ2 integrin) is a conserved phagocytic receptor. The active conformation of binds the iC3b fragment complement C3 as well many host and microbial ligands, leading to actin-dependent phagocytosis. There are conflicting reports about how engagement affects fate phagocytosed substrates. Using imaging flow cytometry, we confirmed that binding internalization iC3b-opsonized polystyrene beads by primary human neutrophils was CR3-dependent. did not stimulate neutrophil...
Neisseria gonorrhoeae (Gc) is a human-specific pathogen that causes the sexually transmitted infection gonorrhea. Gc survives in neutrophil-rich gonorrheal secretions, and recovered bacteria predominantly express phase-variable, surface-expressed opacity-associated (Opa) proteins (Opa+). However, expression of Opa like OpaD decreases survival when exposed to human neutrophils ex vivo. Here, we made unexpected observation incubation with normal serum, which found inflamed mucosal enhances...
Neisseria gonorrhoeae infection is characterized by local and abundant recruitment of neutrophils. Despite neutrophils' antimicrobial activities, viable N. recovered from infected individuals, leading to the question how survives neutrophil attack. One feature impacting gonorrhoeae-neutrophil interactions phase-variable opacity-associated (Opa) proteins. Most Opa proteins engage human carcinoembryonic antigen-related cell adhesion molecules (CEACAMs) facilitate bacterial binding invasion....
The bacterial pathogen Neisseria gonorrhoeae is an urgent global health problem due to increasing numbers of infections, coupled with rampant antibiotic resistance. Vaccines against gonorrhea are being prioritized combat drug-resistant N. gonorrhoeae. Meningococcal serogroup B vaccines such as four-component meningococcal vaccine (4CMenB) predicted by epidemiology studies cross-protect individuals from natural infection and elicit antibodies that cross-react Evaluation candidates for...
Abstract Human carcinoembryonic antigen‐related cell adhesion molecules (CEACAMs) are a family of receptors that mediate intercellular interactions. Pathogenic bacteria have ligands bind CEACAMs on human cells. Neisseria gonorrhoeae (Gc) encodes numerous unique outer membrane opacity‐associated (Opa) proteins for one or more CEACAMs. expressed epithelial cells facilitate Gc colonization, while those neutrophils affect phagocytosis and consequent intracellular survival Gc. Since Opa protein...
ABSTRACT Introduction Bloodstream infections are a significant threat to soldiers wounded in combat and contribute preventable deaths. Novel combination therapies that can be delivered on the battlefield or lower roles of care urgently needed address bloodstream infection among military personnel. In this manuscript, we tested antibacterial capability silver ions (Ag+), with long-appreciated properties, against ESKAPEE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae,...
ABSTRACT Introduction Extensive trauma, commonly seen in wounded military Service Members, often leads to a severe sterile inflammation termed systemic inflammatory response syndrome (SIRS), which can progress multiple organ dysfunction (MODS) and death. MODS is serious threat historically causing 10% of all deaths trauma admissions at forward deployed combat hospital. The importance this problem will be exacerbated large-scale operations, evacuation delayed care complex injuries lower...
Abstract The bacterial pathogen Neisseria gonorrhoeae is an urgent global health problem due to increasing numbers of infections, coupled with rampant antibiotic resistance. Vaccines against gonorrhea are being prioritized combat drug-resistant N. gonorrhoeae. Meningococcal serogroup B vaccines such as 4CMenB predicted by epidemiology studies cross-protect individuals from natural infection and elicit antibodies that cross-react Evaluation vaccine candidates for requires a suite assays...
ABSTRACT Complement receptor 3 (CR3; CD11b/CD18; α m β 2 integrin) is a conserved phagocytic receptor. The active conformation of CR3 binds the iC3b fragment complement C3 as well many host and microbial ligands, leading to actin-dependent phagocytosis. There are conflicting reports about how engagement affects fate phagocytosed substrates. Using imaging flow cytometry, we confirmed that binding internalization iC3b-opsonized polystyrene beads by primary human neutrophils was CR3-dependent....
Abstract Neisseria gonorrhoeae (Gc) is a human-specific pathogen that causes the sexually transmitted infection gonorrhea. Gc survives in neutrophil-rich gonorrheal secretions, and recovered bacteria predominantly express phase-variable, surface-expressed opacity-associated (Opa) proteins (Opa+). However, expression of Opa like OpaD decreases survival when exposed to human neutrophils ex vivo . Here, we made unexpected observation incubation with normal serum, which found inflamed mucosal...