ABSTRACT Cell proliferation in the gastrulating chick embryo was assessed using two independent techniques which mark cells S phase of mitotic cycle: nuclear incorporation bromodeoxyuridine (BrdU) detected immunocytochemically and immunolocalization proliferating cell antigen (PCNA). Computer-reconstructed maps were produced showing distribution labelled nuclei primitive streak layers. These distributions also normalized to take into account regional differences density across embryo....

Type-1 diabetes (T1D) is a metabolic disorder associated with massive reduction in mass of adipose tissue. Measuring cell diameter, an index fat metabolism, we determined depot-specific alterations weight tissue, diameter and size heterogeneity distribution at 5 depots streptozotocin (STZ)-induced diabetic rats. T1D was induced by single injection STZ. Seven days after the injection, were isolated, weighted, washed maintained tissue culture medium. Using microscope equipped calibrated...

Cytoskeleton not only controls cell morphology but also regulates growth, migration, differentiation, and gene expression, events which are fundamental to embryogenesis, carcinogenesis, wound healing. We have recently reported that reorganization of cytoskeleton induces expression mRNA for transforming growth factor-beta1 (TGF-β1), collagenase, tissue inhibitor metalloproteinase-I (TIMP-I) in dermal fibroblasts. In this report we examined the role transcription induction. As judged by...

We have previously reported that C-peptide modulates insulin-mediated inhibition of lipolysis and glucose consumption but has no significant effects per se on adipose tissue normal rats. It been repeatedly observed certain actions are restricted to the diabetic states. In present study, therefore, we examined whether alters in Rats were rendered by streptozotocin divided into 2 groups; insulin treated untreated. Retroperitoneal was excised aseptically, subjected organ culture incubated with...

Several mechanisms have been proposed to explain berries' anticancer effects. However, no previously published studies investigated anti-telomerase activity. In this study, the activity of blackberry crude extract was analyzed in six human colorectal cancer (CRC) cell lines by TRAP assay. The peripheral blood mononuclear cells (PBMCs) from a healthy donor were used as normal control. We also examined effect on telomerase RNA (hTR) mRNA level and reverse transcriptase (hTERT) expression...

A severe thermal injury is commonly associated with immune suppression and increased susceptibility to sepsis, frequently leading multiple organ failure. Transforming growth factor-beta (TGF-beta) a potent immunosuppressive cytokine involved in complications major trauma. Interleukin- 4 (IL-4) thought synergize the activity of TGF-beta by promoting naive lymphocytes differentiate generate secreting cells. This study examines alterations serum levels IL-4 after injury. Male Sprague-Dawley...

Fruit-derived polyphenolic compounds have been shown to exert anticancer effects via epigenetic mechanisms. In this study, we investigated the effect of blackberry extract on expression DNMTs (Dnmt1, Dnmt3a, and Dnmt3b) HDACs (HDAC1-4 SIRT1) its influence cellular differentiation promoter DNA methylation tumor-related genes using a panel six human CRC cell lines. Treatment with IC20 IC50 concentrations for 72 h significantly reduced Dnmt1 Dnmt3b transcript levels in HCT116, SW480, HT29/219,...

Existing data show that C-peptide (CP) prevents or ameliorates diabetes-related complications mainly by improving microcirculation and perhaps metabolism. Although effects of CP on muscle glucose consumption are relatively well studied, its adipose tissue, a key organ involved in metabolism, not known. Therefore, the aim this study was to examine basal stimulated lipolysis rat retroperitoneal (RP) using an EX-VIVO culture setting. The RP tissue excised from adult male rats, minced subjected...

The effects of a 60% body surface area thermal injury in rats on the morphology and proliferation epithelium small intestine vitro serum collected from scalded intestinal epithelial cells were investigated. Scald caused significant reductions duodenal villus width crypt dimensions, enterocytes changed shape columnar to cuboidal, number goblet decreased. proportion bromodeoxyuridine-labeled S phase crypts was also diminished. In vitro, incubation (IEC-6) with rat (SRS) at either 12 or 24 h...

We examined the effects of thermal injury on intestinal epithelial cell proliferation and death. recorded histologically identifiable mitotic apoptotic crypt cells in relation to position after a 60% full thickness cutaneous rat. The significantly reduced mitosis (0.53 ± 0.11 vs. 1.50 0.70, P < 0.05) at positions 4–6, stem cells, 6 h injury. A similar reduction (1.13 0.59 3.50 0.80, was observed higher 7–9 12 injury, indicating positional shift. In addition, significant increase number...

Objective: Available data from in vitro studies show that thyroid hormones (THs) regulate herpes simplex virus (HSV) gene expression and may modulate latency/reactivation of the virus. Whether infectivity is also affected by THs not known. Using animal models (in vivo study) Vero cell culture study), we examined effects alterations level on HSV-1 infectivity. Methods: Rats were rendered hypo- hyperthyroid daily addition methimazole l-thyroxine into their drinking water, respectively....

Extracellular matrix serves as a scaffold for cells and can also regulate gene expression ultimately cell behaviour. In this study, we compared the effects of three forms type I collagen matrix, which differed only in their mechanical properties, plastic on transforming growth factor-beta1 (TGF-beta1), metalloproteinase-1 (collagenase), survival human dermal fibroblasts. These were correlated with alterations morphology organization intracellular actin. Cells detached or stress-relaxed...

Deregulation of Thyroid Hormones (THs) system in Colorectal Cancer (CRC) suggests that these hormones may play roles CRC pathogenesis. Flavonoids are polyphenolic compounds, which possess potent antitumor activities and interfere, albeit some them, with all aspects THs physiology. Whether the actions flavonoids affected by is unknown. Therefore, we investigated effects apigenin (Api), a well-known flavone, on tumorigenic properties SW480 cells presence absence L-thyroxine (T4).Cell viability...

Increasingly in recent years, thyroid hormones (THs) have been considered to be important regulators of the immune system. However, their roles host defense against viral infections are not clearly established. Therefore, this study was undertaken examine proliferative activity and cytokine production by lymphocytes isolated from hyperthyroid euthyroid Balb/c mice response herpes simplex virus-1 (HSV-1). Lymphocytes animals showed a significantly higher rate proliferation interferon (IFN)-γ...

Extracellular matrix serves as a scaffold for cells and can also regulate gene expression ultimately cell behaviour. In this study, we compared the effects of three forms type I collagen matrix, which differed only in their mechanical properties, plastic on transforming growth factor-β1 (TGF-β1), metalloproteinase-1 (collagenase), survival human dermal fibroblasts. These were correlated with alterations morphology organization intracellular actin. Cells detached or stress-relaxed matrices...

Trauma and ventilator-induced lung injury is often associated with endothelial-epithelial barriers breakdown, which may lead to multiple system organ failure (MSOF) death in critically ill patients. Although molecular mechanism involved MSOF not known, junctional opening believed happen. In vitro, thyroid hormones inhibit myosin light chain (MLC) phosphorylation may, thus, cellular contraction opening. also tissue hypo-thyroid state. Therefore, we examined the effects of function on...

Severe burn injury is commonly associated with significant changes in intestinal epithelial function. These include mucosal atrophy and increased permeability. To date, the mechanism by which alters epithelium function are not clear. We used an vitro model of epithelium, IEC-6 cells, observed that integrity confluent culture disrupted cell growth migration rates reduced presence serum collected from scald rats (SRS) (6). identify gene products involved mechanisms underlying these effects, we...