A5039994497- Reproductive Physiology in Livestock
- MicroRNA in disease regulation
- Rabbits: Nutrition, Reproduction, Health
- Circular RNAs in diseases
- Reproductive System and Pregnancy
- Cancer-related molecular mechanisms research
- Adipose Tissue and Metabolism
- Genetic and phenotypic traits in livestock
- Human-Animal Interaction Studies
- Veterinary Oncology Research
- Molecular Biology Techniques and Applications
- Reproductive Biology and Fertility
- Stress Responses and Cortisol
- Animal health and immunology
- Regulation of Appetite and Obesity
- Meningioma and schwannoma management
- Animal Behavior and Welfare Studies
- Veterinary Equine Medical Research
- Oral and Maxillofacial Pathology
- RNA modifications and cancer
- Apelin-related biomedical research
- High Altitude and Hypoxia
- Cardiovascular Conditions and Treatments
- Cardiomyopathy and Myosin Studies
- RNA Interference and Gene Delivery
University of Perugia
2016-2025
Wellcome Centre for Infectious Diseases Research in Africa
2017
University of Cape Town
2017
Institute of Social and Preventive Medicine
2017
University of Bern
2017
Università di Camerino
2002
Introduction: Diagnosis of prostate cancer (PCa) is based on biopsy that performed when specific antigen (PSA) persistently altered over time and/or abnormal digital rectal examination found. Serum PSA levels increase in both PCa and benign prostatic hyperplasia, leading to an increased number unnecessary biopsies. There urgent need unravel PCa-specific molecular signatures. Patients methods: This study aimed at characterizing a panel circulating microRNAs (miRNAs) could distinguish from...
There is emerging evidence that microRNAs (miRNAs) dysregulation involved in the genesis and progression of Prostate Cancer (PCa), thus potentially increasing their use urological clinical practice. This first pilot study which utilizes Illumina Deep Sequencing to examine entire miRNAs spectrum existent urine exfoliated prostate cells (UEPCs) PCa patients. A total 11 male patients with histological diagnosis were enrolled present study. First-catch (30 mL) was collected following a massage....
Ghrelin (GhRL) is an orexigenic hormone influenced by nutritional state. It plays a role in skin repair and diseases, though little information exists regarding its function this organ. GhRL receptor were investigated the of sheep under different feeding conditions to explore system presence possible modifications due diet. Three-year-old female free graze from June pasture maximum flowering (MxF group) period dryness addicted (Exp or not (MxD with 600 gr/die/head barley corn. Skin samples...
Nerve growth factor (NGF) plays a critical role in reproduction through paracrine and endocrine mechanisms. However, its autocrine effects on uterine receptivity inflammatory pathways remain unknown. This study is the first to demonstrate NGF's direct action sheep endometrial luminal epithelial cells (SELECs), primary cultures treated with NGF for 12, 24, 48 h, or without NTRK1 antagonist. significantly increased PGE2 (p < 0.0001) PGF2α levels only at 12 no significant changes 24 h. also...
Inflammatory bowel disease (IBD) is a chronic inflammatory disorder for which the current medical therapy not completely effective. Bovine colostrum (BC) biological fluid rich in bioactive molecules that may have beneficial effects on several gastrointestinal disorders. The objectives of this study were to assess preventive BC supplementation mouse model 2,4,6 trinitrobenzene sulfonic acid (TNBS)-induced colitis using multidisciplinary approach. Specifically, following parameters evaluated:...
This study aims to define the personality traits perceived by owners of multiple pets and evaluate how they are modulated experiential-environmental factors. A questionnaire was administered 1270 (dogs cats) collect data on demographics, management, their pets. Data were analysed principal component analysis, bivariate, multivariable models. Five emerged in dogs cats: sociability, reactivity, protectiveness, neuroticism, fearfulness. The differences pet: scored higher while lower neuroticism...
The aim of our study was to monitor serum levels two miRNAs (miR-21 and miR-141) three KLKs (hK3/PSA, hK11, hK13) before 1, 5, 30 days after radical prostatectomy, in order characterize their fluctuations surgery. 38 patients with prostate cancer were included. miR-21 miR-141 quantified through real-time PCR, while ELISA assays used quantify hK3 (PSA), hK13. Both showed a significant increase at the 5th postoperative day, which gradual return preoperative recorded. These findings suggest...
We aimed at assessing the stability of candidate reference genes in urine sediments men subjected to digital rectal examination for suspected prostate cancer (PCa). Two microRNAs (miR-191 and miR-25) 1 small nucleolar RNA (SNORD48) were assayed 35 post-DRE with PCa 26 subjects histologically confirmed benign prostatic hyperplasia (BPH). The was assessed through BestKeeper algorithm equivalence test. miR-200b miR-452 used test effect normalization on target genes. Our results proved miR-191...
MiRNA-based therapies represent an innovative and promising strategy applicable to various medical fields, such as tissue regeneration the treatment of numerous diseases, including cancer, cardiovascular problems, viral infections. MiRNAs, a group small non-coding RNAs, play critical role in regulating gene expression at post-transcriptional level modulate several signaling pathways that maintain cellular homeostasis. The clinical trials discussed review herald new therapeutic era for...
The expression of type I GNRH receptor (GNRHR-I) and the direct role GNRH-I on corpora lutea (CL) function were studied in pseudopregnant rabbit model. Immunohistochemistry evidenced GNRHR-I luteal cells at early (day 4 pseudopregnancy)-, mid 9)-, late 13)-luteal stages. Real-time RT-PCR western blotting revealed mRNA protein three Buserelin vivo treatment days 9 13 decreased plasma progesterone levels for 48 24 h respectively. In vitro cultured CL, buserelin reduced secretion, increased...
CPV1 (also called COPV) is a papillomavirus responsible for oral papillomatosis in young dogs. The involvement of this viral type oncogenesis has been hypothesized squamous cell carcinomas (SCCs), but never investigated other neoplastic and hyperplastic lesions Aim study was to investigate the presence different order assess its role canine oncogenesis; according results obtained, second aim define if dog can be considered valid animal model high risk HPV-induced tumors. Eighty-eight...
A high standard of physical fitness is an essential characteristic drug detection dogs because it affects not only their ability to sustain activity levels but also attention and olfaction efficiency. Nutritional supplements could improve by modulating energy metabolism, oxidative processes, perceived fatigue. The aim this study was investigate the physiological biochemical changes induced submaximal exercise on (German Shepherd breed) assess whether a dietary supplement improves fitness....
Studies were conducted to characterize receptors for prostaglandin (PG) F(2alpha) (PGF(2alpha)) and PGE(2), the signalling pathways regulating total nitric oxide synthase activity progesterone production in rabbit corpora lutea (CL) of different luteal stages. CL obtained at days 4, 9 13 pseudopregnancy cultured vitro 2 h with PGF(2alpha) or PGE(2) activators inhibitors G protein (Gp), phospholipase C (PLC), kinase (PKC), adenylate cyclase (AC) A (PKA). High affinity receptor (K(d)=1.9+/-0.6...
The aim of this study was to better understand the role endothelin-1 (ET-1) system in process controlling corpora lutea (CL) life span rabbits. ET-1 (10 microg iv) administration at d 9 and 12 pseudopregnancy induced a functional luteolysis within 24 h injection, but it ineffective both 4 6. Pretreatments with Bosentan, dual ET(A)/ET(B) receptor antagonist, or cyclooxygenase (COX) inhibitor blocked luteolytic action not that by prostaglandin F2alpha (PGF2alpha). In CL cultured vitro,...
This study investigated the effects of a short-term administration bovine colostrum (BC) in TNBS model induced colitis. Colitis was by treatment after seven days BC (BC group, n = 12) or saline (control mice. Clinical signs, histopathological characteristics, expression levels Toll-like receptor 4 (TLR4), pro- and anti-inflammatory cytokines, microbial composition were assessed. well tolerated did not induce any histological damage clinical symptoms. After treatment, group showed reduction...
The gene expressions for macrophage chemoattractant protein-1 (MCP-1), interleukin (IL)-1β, IL-2 and p53 were examined by semi-quantitative RT-PCR in corpora lutea (CL) of rabbits during spontaneous luteolysis at days 13, 15, 18 22 pseudopregnancy. In the same luteal tissue, total activity nitric oxide (NO) synthase (NOS) genes both endothelial (eNOS) inducible (iNOS) isoforms also analysed. From day 13 to MCP-1 IL-1βmRNA levels rose ( P ≤ 0.01) almost 2-fold, transcript 8-fold, but then all...