A5050346339- Veterinary Oncology Research
- Virus-based gene therapy research
- Infectious Diseases and Mycology
- Cancer Cells and Metastasis
- Oral and Maxillofacial Pathology
- Microbial infections and disease research
- Skin and Cellular Biology Research
- Nail Diseases and Treatments
- Sarcoma Diagnosis and Treatment
- Immunotherapy and Immune Responses
- Cervical Cancer and HPV Research
- Herpesvirus Infections and Treatments
- Bone Tumor Diagnosis and Treatments
- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- Hedgehog Signaling Pathway Studies
- Cancer and Skin Lesions
- Tumors and Oncological Cases
- melanin and skin pigmentation
- Genital Health and Disease
- Cutaneous lymphoproliferative disorders research
- Cell Adhesion Molecules Research
- T-cell and B-cell Immunology
- T-cell and Retrovirus Studies
- Wnt/β-catenin signaling in development and cancer
University of Perugia
2016-2025
University of Illinois Urbana-Champaign
2004
Istituto Superiore di Sanità
1986-1993
Moredun Research Institute
1993
In humans, the presence of an even distribution melanocytes within epidermal basal layer allows for uniform pigmentation in healthy and young individuals. Moreover, despite high variability skin colours tones, interindividual melanocyte density is low. However, dogs display a intraindividual pigmentary different anatomical areas.
Aging involves progressive physiological changes, including the dysregulation of water homeostasis, essential for cellular function, neuronal signaling, and musculoskeletal integrity. This review explores emerging role loss as a central underestimated driver functional decline in aging, with focus on dog, both clinically relevant target species model human aging. Age-related alterations metabolism—driven by changes body composition, aquaporin (AQP) expression, electrolyte imbalances, reduced...
Feline injection-site sarcoma (FISS) is an aggressive tumor believed to arise from the proliferation of fibroblasts and myofibroblasts in areas chronic inflammation, particularly at sites injection. Local recurrence frequent after surgical excision. Gelatinases (MMP-2 MMP-9) their inhibitor (TIMP-2) are endopeptidases pivotal extracellular matrix remodeling therefore invasiveness. The aim this study was investigate immunohistochemical expression MMP-2, MMP-9, TIMP-2 FISS assess usefulness as...
The aim of our study was to monitor serum levels two miRNAs (miR-21 and miR-141) three KLKs (hK3/PSA, hK11, hK13) before 1, 5, 30 days after radical prostatectomy, in order characterize their fluctuations surgery. 38 patients with prostate cancer were included. miR-21 miR-141 quantified through real-time PCR, while ELISA assays used quantify hK3 (PSA), hK13. Both showed a significant increase at the 5th postoperative day, which gradual return preoperative recorded. These findings suggest...
Abstract The study of the immune response in several types tumours has been rapidly increasing recent years with dual aim understanding interactions between neoplastic and cells their importance cancer pathogenesis progression, as well identifying targets for immunotherapy. Despite being considered one most immunogenic tumour types, melanoma can progress presence abundant lymphocytic infiltration, therefore suggesting that is not able to efficiently control growth. purpose this was...
Despite promising immunotherapy strategies in human melanoma, there are few studies on the immune environment of canine melanocytic tumors. In humans, activation immunosuppressive cell subpopulations, such as regulatory T cells (Tregs) that express forkhead box protein P3 (FoxP3), engagement surface receptors like cytotoxic lymphocyte antigen (CTLA-4), and secretion molecules inhibiting activation, indoleamine-pyrrole 2,3-dioxygenase (IDO), recognized immunoescape mechanisms allow tumor...
The tumor microenvironment is a complex system, where neoplastic cells interact with immune and stromal cells. Tumor-associated macrophages (TAMs) are considered among the most numerically biologically noteworthy cellular components in tumors attention on this population has been growing during last decade, both for its prognostic role as potential future therapeutic target. Melanoma, particularly oral form, despite being one of immunogenic tumors, bears poor prognosis dogs humans, due to...
Interactions between tumor cells and microenvironment are considered critical in carcinogenesis, invasion metastasis. To examine transcriptome changes to explore the relationship with canine cutaneous melanocytoma melanoma, we extracted RNA from formalin-fixed, paraffin-embedded (FFPE) specimens analyzed them by means of RNA-seq for transcriptional analysis. Melanocytoma melanoma samples were compared detect differential gene expressions significant enriched pathways explored reveal...
Human melanoma is one of the deadliest forms cancer, with poor prognosis and high resistance to chemotherapy radiotherapy. The discovery immunosuppressive mechanisms in human microenvironment led use new prognostic markers development immunotherapies targeting immune checkpoint molecules. Immunoescape canine have not yet been investigated, no such immunotherapy has tested. aim this study was provide preliminary data on expression transcription factor forkhead box protein P3 (FoxP3)...
CPV1 (also called COPV) is a papillomavirus responsible for oral papillomatosis in young dogs. The involvement of this viral type oncogenesis has been hypothesized squamous cell carcinomas (SCCs), but never investigated other neoplastic and hyperplastic lesions Aim study was to investigate the presence different order assess its role canine oncogenesis; according results obtained, second aim define if dog can be considered valid animal model high risk HPV-induced tumors. Eighty-eight...
Equine penile squamous cell carcinoma (epSCC) is the most frequent tumor of external male genitalia, representing 67.5% equine genital cancers. epSCC associated with papilloma virus (PV) infection and has been recently proposed as a model for human PV-induced carcinomas. It already suggested that might undergo epithelial-to-mesenchymal transition (EMT). This work aims to investigate in detail this process possible role PV oncoproteins epSCC. For purpose, 18 SCCs were retrospectively selected...
Papillomaviruses (PVs) are small, non-enveloped viruses, ubiquitous across the animal kingdom. PVs induce diverse forms of infection, such as cutaneous papillomas, genital papillomatosis, and carcinomas. During a survey on fertility status mare, novel Equus caballus PV (EcPV) has been identified using Next Generation Sequencing, it was further confirmed with genome-walking PCR Sanger sequencing. The complete circular genome 7607 bp long shares 67% average percentage identity EcPV9, EcPV2,...
A 14-year-old female spayed Dachshund was presented with generalized scaling, erythema, pruritus, poor quality of hair coat, and progressive weight loss. Cutaneous epitheliotropic T-cell lymphoma (CETCL) suspected. Skin biopsies were suggestive CETCL. However, immunohistochemistry revealed the presence numerous CD20+ CD3+ cells. Clonality assay demonstrated a clonal receptor gamma rearrangement polyclonal IgH gene rearrangement. Double-label immunofluorescence confirmed coexpression CD3 CD20...
Breslow thickness and Clark level are prognostic factors for human cutaneous melanomas. is measured with an ocular micrometer from the top of granular layer epidermis to deepest invasive cell across broad base tumor, while based on anatomical invasion through layers dermis. Because differences between humans dogs, we evaluated tumor a modified in 77 canine primary melanocytic tumors. Tumor (using both traditional more convenient system) were associated histological diagnosis clinical...
Background – Although cutaneous stem cells have been implicated in skin tumourigenesis humans, no studies conducted to elucidate the presence and possible role of hair follicle tumours dog. Hypothesis Stem cell markers are expressed canine epidermal follicular can be used better understand biology origin these tumours. Animals Methods In present study, normal sections 44 were retrospectively investigated for immunohistochemical expression keratin 15 (K15) nestin. addition, 30 squamous...
Equine genital squamous cell carcinomas (egSCCs) are among the most common equine tumors after sarcoids, severely impairing animal health and welfare. Equus caballus papillomavirus type 2 (EcPV2) infection is often related to these tumors. The aim of this study was clarify molecular mechanisms behind egSCCs associated with EcPV2 infection, investigating receptor activator nuclear factor-kappa B ligand (RANKL) signaling in NF-kB pathway, together Wnt IL17 pathways. We analyzed innate immune...
Melanoblasts originate in the neural crest from where they migrate to peripheral tissues and differentiate into melanocytes. Alteration during melanocyte development life can cause different diseases, ranging pigmentary disorders decreased visual auditory functions, tumours such as melanoma. Location phenotypical features of melanocytes have been characterised species, yet data on dogs are lacking.This study investigates expression melanocytic markers Melan A, PNL2, TRP1, TRP2, SOX-10 MITF...
Zinc deficiency causes skin diseases both in humans and animals. The underlying pathogenic mechanisms remain unclear, but a growing body of evidence indicates role for zinc protection against free radical-induced oxidative damage. immunohistochemical expression heat shock proteins (HSPs; Hsp27, Hsp72, Hsp73 Hsp90), Cu/Zn superoxide dismutase (SOD), metallothionein (MT), Ki-67 antigen active caspase-3 were evaluated normal canine samples from eight dogs with zinc-responsive dermatosis. All...