A5063991133- Calcium signaling and nucleotide metabolism
- Autophagy in Disease and Therapy
- PARP inhibition in cancer therapy
- Toxin Mechanisms and Immunotoxins
- Lysosomal Storage Disorders Research
- Cardiac Valve Diseases and Treatments
- Cellular transport and secretion
- Parkinson's Disease Mechanisms and Treatments
- RNA Interference and Gene Delivery
- Microtubule and mitosis dynamics
- Lipid metabolism and biosynthesis
- Metabolism, Diabetes, and Cancer
- Genetic Neurodegenerative Diseases
- Adipose Tissue and Metabolism
- Photoreceptor and optogenetics research
- Plant nutrient uptake and metabolism
- Electrostatic Discharge in Electronics
- Ion Channels and Receptors
- Piperaceae Chemical and Biological Studies
- Photosynthetic Processes and Mechanisms
- Cardiac tumors and thrombi
- Alzheimer's disease research and treatments
- Cardiac and Coronary Surgery Techniques
- Congenital Heart Disease Studies
- Plant responses to water stress
Institute for Experimental Endocrinology and Oncology
2022-2024
University of Toronto
2024
Telethon Institute Of Genetics And Medicine
2015-2024
National Research Council
2022-2023
Harvard University
2021
Boston Children's Hospital
2021
Mario Negri Sud Foundation
2010-2014
Abstract The lysosomal calcium channel TRPML1, whose mutations cause the storage disorder (LSD) mucolipidosis type IV (MLIV), contributes to upregulate autophagic genes by inducing nuclear translocation of transcription factor EB (TFEB). Here we show that TRPML1 activation also induces vesicle (AV) biogenesis through generation phosphatidylinositol 3-phosphate (PI3P) and recruitment essential PI3P-binding proteins nascent phagophore in a TFEB-independent manner. Thus, formation requires...
Background Protein mono-ADP-ribosylation is a reversible post-translational modification that modulates the function of target proteins. The enzymes catalyze this reaction in mammalian cells are either bacterial pathogenic toxins or endogenous cellular ADP-ribosyltransferases. latter include members three different families proteins: well characterized arginine-specific ecto-enzymes ARTCs, two sirtuins and, more recently, novel poly(ADP-ribose) polymerase (PARP/ARTD) family have been...
Abstract Selective removal of dysfunctional mitochondria via autophagy is crucial for the maintenance cellular homeostasis. This event initiated by translocation E3 ubiquitin ligase Parkin to damaged mitochondria, and it requires Serine/Threonine-protein kinase PINK1. In a coordinated set events, PINK1 operates upstream in linear pathway that leads phosphorylation Parkin, Ubiquitin, mitochondrial substrates, promote ubiquitination outer membrane proteins. Ubiquitin-decorated are selectively...
Intracellular mono-ADP-ribosyltransferases (mono-ARTs) catalyze the covalent attachment of a single ADP-ribose molecule to protein substrates, thus regulating their functions. PARP10 is soluble mono-ART involved in modulation intracellular signaling, metabolism and apoptosis. also participates regulation G1- S-phase cell cycle. However, role this enzyme G2/M progression not defined. In study, we found that genetic ablation, depletion pharmacological inhibition cause delay transition...
Mono-ADP-ribosylation is a reversible post-translational modification that can modulate the functions of target proteins. We have previously demonstrated β subunit heterotrimeric G proteins endogenously mono-ADP-ribosylated, and once modified, βγ dimer inactive toward its effector enzymes. To better understand physiological relevance this modification, we studied hormonal regulation. Here, report Gβ mono-ADP-ribosylation differentially modulated by protein-coupled receptors. In intact cells,...
An established hallmark of cancer cells is metabolic reprogramming, largely consisting in the exacerbated glucose uptake. Adipocytes tumor microenvironment contribute toward breast (BC) progression and are highly responsive to fluctuations. Metabolic conditions characterizing obesity and/or diabetes associate with increased BC incidence mortality. To explore BC-adipocytes interaction define impact such dialogue, Mammary Adipose-derived Mesenchymal Stem Cells (MAd-MSCs) were differentiated...
Abstract The GTPase Rab14 is localized at the trans-Golgi network and intermediate compartment associated to sorting/recycling endosomes-like structures of transferrin-recycling pathway: as other Rab family members, it involved in regulation intracellular vesicle trafficking, though its role functional relationship with effector/endosomal proteins still incomplete. We have analysed whether post-translational modifications could affect activity: results obtained define mono-ADP-ribosylation...
Abstract Selective removal of dysfunctional mitochondria via autophagy is crucial for the maintenance cellular homeostasis. This event initiated by translocation E3 ubiquitin ligase Parkin to damaged mitochondria, and it requires Serine/Threonine-protein kinase PINK1. In a coordinated set events, PINK1 operates upstream in linear pathway that leads phosphorylation Parkin, Ubiquitin, mitochondrial substrates, promote ubiquitination outer membrane proteins. Ubiquitin decorated are selectively...