The prevalence of large bone defects is still a major problem in surgical clinics. It is, thus, not surprise that bone-related research, especially the field tissue engineering, issue medical research. Researchers worldwide are searching for missing link engineering graft materials mimic bones, and foster osteogenesis remodeling. One approach combination additive manufacturing technology with smart additionally electrically active biomaterials. In this study, we performed three-dimensional...

The treatment of critical size bone defects, for example resulting from large tumor resections, still remains a challenge in clinical practice. It has been demonstrated that the gold standard based on auto- and allografts can provide aid some cases. However, development patient specific 3D printed implants modern biomaterial-based tissue engineering (TE) strategies, avoiding requirement harvesting donor site, promises to be next generation healing. We present PLA-Bioactive glass (BG)...

Electroactive hydrogels can be used to influence cell response and maturation by electrical stimulation. However, hydrogel formulations which are 3D printable, electroactive, cytocompatible, allow adhesion, remain a challenge in the design of such stimuli-responsive biomaterials for tissue engineering. Here, combination pyrrole with high gelatin-content oxidized alginate-gelatin (ADA-GEL) is reported, offering 3D-printability precursors prepare cytocompatible electrically conductive...

Three-dimensional (3D) printing technology enables the design of personalized scaffolds with tunable pore size and composition.Combining decellularization 3D techniques provides opportunity to fabricate high potential mimic native tissue.The aim this study is produce novel decellularized bone extracellular matrix (dbECM)-reinforced composite-scaffold that can be used as a biomaterial for tissue engineering.Decellularized particles (dbPTs, ~100 μm diameter) were obtained from rabbit femur...

Bone healing is a complex process orchestrated by various factors, such as mechanical, chemical and electrical cues. Creating synthetic biomaterials that combine several of these factors leading to tailored controlled tissue regeneration, the goal scientists worldwide. Among those piezoelectricity which creates physiological microenvironment plays an important role in stimulating bone cells fostering regeneration. However, only limited number studies have addressed potential combining...

Composite Extrusion Modeling (CEM) is a screw-based material extrusion (MEX) additive manufacturing process that can produce plastic, metal and ceramic parts based on standard injection molding feedstocks. In this work, an aluminum oxide feedstock (Al2O3) originally developed for processed via MEX the first time by depositing plasticized in layers build platform. order to identify appropriate parameters, characterized estimate printable temperature processing window. Initially, best values...

Additive manufactured porous biomaterials based on triply periodic minimal surfaces (TPMS) are a highly discussed topic in the literature. With their unique properties terms of open porosity, large surface area and curvature, they considered to have bone mimicking remarkable osteogenic potential. In this study, scaffolds gyroid unit cells different sizes consisting Ti6Al4V alloy were additively by electron beam melting (EBM). The analysed micro-computed tomography (micro-CT) determine...

The effect of chemical solvents on the wetting state laser-structured surfaces over time is systematically examined in this paper. By using a 300-fs laser, nanostructures were generated Ti6Al4V, subsequently cleaned an ultrasonic bath with different and stored ambient air. static contact angle showed significant differences for cleaning various solvents, which, depending applied time, amounted up to 100°. X-ray photoelectron spectroscopy analyses reveal that affects surface chemistry aging...

This study presents a detailed characterization of self-organized nano- and microstructures on Ti6Al4V evoked by different scanning strategies fluences with 300 fs laser operating at wavelength 1030 nm. The resulting surface morphology was visualized via field emission electron microscopy (FEG-SEM) images the cross-sections. X-ray diffraction (XRD)-analysis performed to analyse changes in crystal structures. chemical composition near-surface layer determined photoelectron spectroscopy (XPS)....

ABSTRACT Caloric restriction (CR) is a well-described dietary intervention that delays the onset of aging-associated biochemical and physiological changes, thereby extending life span rodents. The influence CR on metabolism, strength, morphology bone has been controversially discussed in literature. Thus, present study evaluated whether lifelong versus short-term late-onset differentially affects development senile osteoporosis C57BL/6 mice. Two different regimens with 40% food were...

Dietary phosphorus frequently exceeds age-specific requirements and pig manure often contains high load which causes environmental burden at regional scales. Therefore, feeding strategies towards improved efficiency reduced have to be developed. A 5-week trial was conducted: piglets received medium, lower (−25%), or higher (+25%) amounts of calcium. responses were reflected by performance parameters, bone characteristics, molecular data retrieved from serum, intestinal mucosa, kidney cortex...

Phosphorus (P) is of vital importance for many aspects metabolism, including bone mineralization, blood buffering, and energy utilization. In order to identify molecular routes affecting intrinsic P utilization, we address processes covering intake, uptake, excretion. particular, the interrelation tissue immune features interest approximate intake animal's physiology health status. German Landrace piglets received different levels digestible phosphorus: recommended, higher, or lower amounts....

Lateral adhesion forces are a fundamental property of liquid-solid interactions and key aspect dynamic droplet mobility. But, commonly applied conventional wetting analysis is limited to static quasi-static methods cannot resolve spatial interactions. However, mobility assumed be affected by chemical topographic surface inhomogeneities introduced femtosecond laser treatment.In this study, we used customized force instrument determine lateral on various laser-structured designs obtain deeper...

Phosphorus (P) is an important element of various metabolic and signalling processes, including bone metabolism immune function. To elucidate the routes P homeostasis utilization, a five-week feeding study was conducted with weaned piglets receiving diet recommended amounts Ca (M), or lower (L) higher (H) values constant Ca:P ratio. Routes utilization were deduced via characteristics (MicroCT), genome-wide transcriptomic profiles peripheral blood mononuclear cells (PBMCs), serum mineral...

The performance of two selective electron beam melting operation modes, namely the manual mode and automatic ‘build theme mode’, have been investigated for case a Ti-6Al-4V alloy (45–105 μm average particle size powder) in terms porosity, microstructure, mechanical properties. modes produced notable differences build quality (porosity), properties over sample thickness. number pores were measured using light microscope entire height. A density measurement provided quantitative index global...

Sufficient supply of pigs with calcium (Ca) and phosphorus (P) is essential for animal health welfare during the growth period. However, P content in manure considered as a cause massive environmental problems soil aquatic ecosystems. To complement previous findings, objective this study investigation effects reduced increased Ca supplementation on bone mineralization structure compared current dietary recommendation. Another aim to find possible serum markers that would allow assessment...

A critical-size bone defect in load-bearing areas is a challenging clinical problem orthopaedic surgery. Titanium alloy (Ti6Al4V) scaffolds have advantages because of their biomechanical stability but lack electrical activity, which hinders further use. This work focused on the fabrication Ti6Al4V-Barium Titanate (BaTiO3) bulk composite to combine Ti6Al4V with activity through BaTiO3. For first time, hollow cylindrical additively manufactured by electron beam melting and combined...

Abstract The deep understanding of cancer and tumor genesis, as well the development new therapy strategies still remains one emerging challenges in modern medicine. To meet these it seems to be absolutely necessary overcome drawbacks established 2D vitro models. Especially missing microenvironment tumor, which means absence stroma immune cells, results a cell-cell cell-stroma interaction disrupted functional communication pathways. Modern 3D culture systems printing or rather bioprinting...

The topology-property relationship of porous structures, especially tested under quasi-static and cyclic loadings, is an essential factor for the design safe application structures in medical context. Within scope this paper, mechanical properties damage behavior additively manufactured were investigated fatigue compression tests taking different designs with cubic unit cells into account. influence structure-determining parameters on highlighted. For example, analytically described as a...

The development of electrically active biomaterials, which create electrical cues on demand to foster cellular stimulation, remains a challenge. Here, we utilized Field-assisted sintering approach densify barium titanate and 45S5 bioactive glass piezoelectric potentially composite. By using sintering, aimed fabricate dense specimens, preserving low degree crystallization the keep bioactivity as high possible. Therefore, have varied compositions materials processed them at different...

Additive manufacturing (AM) of medical implants continues to receive broad attention in regenerative medicine. The application smart materials combined with AM represents a new approach enhance the functionality modern tailor patient-specific therapies. Considering bone be material, this study aims applicate barium titanate (BaTiO3) as smart, piezoelectric bone-mimicking material for AM. We present powder-based 3D printing process manufacture cylindrical, porous scaffolds. Subsequent,...

Bone tissue has the ability to self heal, but treatment of critical size defects and limited regenerative process in osteoporosis or avascular necrosis demand special medical attention. The field bone engineering aims develop new patient-specific solutions for repair. In this study, we focus on fabrication degradable subchondral scaffolds made highly loaded Polycaprolactone (PCL) using direct ink writing. Barium titanate (BTO) bioactive glass 45S5 (BG) were used as filler materials tailor...

Abstract Vascularization of bioprinted constructs to ensure sufficient nutrient supply still remains be a significant task in the tissue engineering community. In order mimic functional tissue, it is necessary able print vessels various size scales, which places particularly high demands on 3D printing technology and materials. this preliminary study, we focused production small hollow structures for application units living tissue. To fabricate structures, freeform reversible embedding...

The usage of biodegradable polymers as implant material instead metal is growing interest because no second surgery needed and the healing time shortened. Combining with hydroxyapatite particles leads to implants osteoconductive properties improved mechanical strength. Using 3D printing it possible generate highly structured complex or even individualized based on a patient data set. This work presents preliminary study commercially available poly(L-lactide)/hydroxyapatite composite using...

Additive manufacturing of novel biomaterials with additional stimulating functions represents a promising strategy for bone repair. In this study, systematic investigation the necessary thermal post-treatment 3D printed barium titanate composite ceramics to achieve desired mechanical properties regeneration is presented. The maximum sintering temperature was varied from 1320 °C 1380 in 10 K intervals. After sintering, samples were systematically characterized respect their porosity and...