A5048978906- Viral gastroenteritis research and epidemiology
- Escherichia coli research studies
- Clostridium difficile and Clostridium perfringens research
- Salmonella and Campylobacter epidemiology
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- Virus-based gene therapy research
- CAR-T cell therapy research
- Monoclonal and Polyclonal Antibodies Research
- Animal Virus Infections Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Galectins and Cancer Biology
- Cell Adhesion Molecules Research
- Polydiacetylene-based materials and applications
- Biochemical effects in animals
- Probiotics and Fermented Foods
- Veterinary medicine and infectious diseases
- Helicobacter pylori-related gastroenterology studies
- Bacteriophages and microbial interactions
- Vibrio bacteria research studies
- Free Radicals and Antioxidants
- Stress Responses and Cortisol
- Antimicrobial Peptides and Activities
US Biologic (United States)
2020
University of Kansas
2016-2019
Oklahoma State University
2013-2014
Universidad Nacional Autónoma de México
2007-2012
University of Maryland, Baltimore
2011
Shigella spp. are food- and waterborne pathogens that cause severe diarrheal dysenteric disease associated with high morbidity mortality. Individuals most often affected children under 5 years of age in the developing world. The existence multiple serotypes heterogenic distribution pathogenic strains, as well emerging antibiotic resistance, require development a broadly protective vaccine. All utilize type III secretion system (TTSS) to initiate infection. apparatus (TTSA) is molecular...
Effective chimeric antigen receptor (CAR)-T cell therapy is dependent on optimal culture methods conducive to the activation and expansion of T cells ex vivo, as well infection with CAR. Media formulations used in CAR-T manufacturing have not been optimized for gene delivery, expansion, overall potency. Bioactive components derivatives that support generation functionally-competent progeny long-lasting persistence are largely undefined. Current media rely fetal bovine serum (FBS) or human...
Shigellosis is an important disease in the developing world, where about 90 million people become infected with Shigella spp. each year. We previously demonstrated that type three secretion apparatus (T3SA) proteins IpaB and IpaD are protective antigens mouse lethal pulmonary model. In order to simplify vaccine formulation process development, we have evaluated a design incorporates both of these tested into single polypeptide chain. To determine if this fusion protein (DB fusion) retains...
A pressing need exists for autoimmune disease therapies that act in an antigen-specific manner while avoiding global immunosuppression. Multivalent soluble antigen arrays (SAgAPLP:LABL), designed to induce tolerance a specific multiple sclerosis autoantigen, consist of flexible hyaluronic acid (HA) polymer backbone cografted with copies autoantigen peptide (PLP) and cell adhesion inhibitor (LABL). Previous vivo studies revealed copresentation both signals on HA was necessary therapeutic...
Nontyphoidal Salmonella enterica serotypes (NTS) are the leading cause of hospitalization and death due to foodborne illnesses. NTS costliest pathogens ∼$4 billion annually in health care costs. In Africa, new invasive bacteremia, especially HIV-positive children adults. Current vaccines against S. not broadly protective most directed at typhoid-causing serotypes, NTS. All strains require two type III secretion systems (T3SS) for virulence. The T3SS needle tip protein first translocator...
Shigella spp. are causative agents of bacillary dysentery, a human illness with high global morbidity levels, particularly among elderly and infant populations. infects via the fecal-oral route, its virulence is dependent upon type III secretion system (T3SS). Two components exposed needle tip complex T3SS, invasion plasmid antigen D (IpaD) IpaB, have been identified as broadly protective antigens in mouse lethal pneumonia model. A recombinant fusion protein (DB fusion) was created by...
Helicobacter pylori infects around 50% of the world's population and is associated with diverse pathologies. In most severe cases, bacterium causes peptic ulcers gastric cancer. The interplay between H. host's immune response may help to determine specific outcome infection. To study relationship antibody subclasses variation in recognition, we determined immunoglobulin G1 2 (IgG1 IgG2) titres sera obtained from patients different pylori-associated IgG1 IgG2 were by ELISA 44 diseases (peptic...
Achieving cross-protective efficacy against multiple bacterial strains or serotypes is an important goal of vaccine design. Enterotoxigenic Escherichia coli (ETEC) cause diarrheal disease in underdeveloped nations. We have been interested identifying and characterizing ETEC antigens that generate protective immune responses independent colonization factor (CF) expression. Our previous studies used proteomics to identify the MipA, Skp, ETEC_2479 proteins as effective protecting mice from...
Diarrheal diseases are a major cause of morbidity and mortality worldwide. They most prevalent in settings with inadequate sanitation, poor hygiene contaminated water. An important diarrheal pathogen such is Shigella. No commercially available vaccine exists against shigellosis immunity to the serotype-restricted. We have previously shown that polypeptide fusion Type Three Secretion Apparatus (T3SA) proteins IpaB IpaD (named DBF) was efficacious as Vaccination using different administration...