Aberrant crypt foci (ACF) in the colon of carcinogen-treated rodents are considered to be earliest hallmark carcinogenesis. In present study relationship between a short-term (4 weeks) and medium-term (30 assay was assessed model carcinogenesis induced by dimethylhydrazine (DMH) rat. Six-week-old male Wistar rats were given subcutaneous injections DMH (40 mg/kg) twice week for 2 weeks killed at end 4th or 30th week. ACF scored number, distribution pattern along multiplicity 0.1%...

Abstract Propolis is a honeybee product with several biological and therapeutical properties. Its effect on the process of colon carcinogenesis DNA damage were evaluated in male Wistar rats using aberrant crypt foci (ACF) assay comet assay, respectively. For both tests, animals treated carcinogen 1,2 dimethylhydrazine (DMH, 40 mg/kg, s.c.) for 2 weeks (two injections/week) order to induce ACF. The divided into groups that received propolis (ethanolic extract) at three different doses (10,...

Choline deficiency (CD) increases susceptability of (he rat liver to a number hepatocellular carcinogens with wide diversity structure and potency. While severe CD results in micronodular cirrhosis enhanced tumor induction, even mild deficiency, without is sufficient result the increased carcinogenic response. The effects are part mediated via modulation microsomal and, possibly, cytosolic enzymes responsible for activation/deactivation carcinogens. B6C3F1 hybrid mouse remarkably sensitive...

The Brazilian Agency of Sanitary Vigilance (ANVISA) conducted a study that demonstrated the presence residues several pesticides in fresh fruits and vegetables were available for purchase by general populace. In order to evaluate potential adverse health effects low-level exposure agrochemicals, reproductive toxicity dicofol, dichlorvos, permethrin, endosulfan, dieldrin was evaluated rats dosed with these chemicals individually or as mixtures. Sixty male Lewis (6 wk old, 200 x g) randomly...

Aims To investigate the prognostic value of expression levels genes STEAP 1 and 2, protein, in prostate carcinomas ( PC a). Methods results 2 transcript were evaluated by RT ‐q PCR samples from 35 a, 24 adjacent non‐neoplastic A dj P ) tissues, five cases benign prostatic hyperplasia BPH ), two histologically normal prostates N ). was assessed immunohistochemistry 198 76 djP, 22 , . The findings compared with clinical pathological parameters patient outcome. analysis showed no differences...

Abstract The morphologic appearance and clinical behavior of the human urinary bladder papillary transitional cell carcinoma (TCC) probably result from a complex interaction between carcinogenic insults host resistance during patient’s life. While main recognized risk factors are environmental origin (e.g. smoking), relatively little information exists about susceptibility to TCC development. leukocyte antigen G (HLA‐G) molecule plays an important role in immune response regulation has been...

Glyphosate has been rigorously and extensively tested for carcinogenicity by administration to mice (five studies) rats (nine studies). Most authorities have concluded that the evidence does not indicate a cancer risk humans. The International Agency Research on Cancer (IARC), however, evaluated some of available data glyphosate probably is carcinogenic expert panel convened Intertek assessed findings used IARC, as well full body found following: (1) renal neoplastic effects in males one...

Two wide‐spectrum initiation models were investigated in F344 male rats. Model I: After sequential treatment with diethylnitrosamine (DEN), N‐methylnitrosourea (MNU) and dihydroxy‐di‐N‐propylnitrosamine (DHPN), animals given phenobarbital (PB) or N, N‐dibutylnitrosamine (DBN) as a test compound for 14 weeks sacrificed at week 18. II: Animals treated DHPN, followed by N‐ethyl‐N‐hydroxyethylnitrosamine (EHEN), then 3,2′‐dimethyl‐4‐aminobiphenyl (DMAB) initiators subsequently...

Abstract Background Epigenetic alterations are a hallmark of human cancer. In this study, we aimed to investigate whether aberrant DNA methylation cancer-associated genes is related urinary bladder cancer recurrence. Methods A set 4 genes, including CDH1 (E-cadherin), SFN (stratifin), RARB (retinoic acid receptor, beta) and RASSF1A (Ras association (RalGDS/AF-6) domain family 1), had their patterns evaluated by MSP (Methylation-Specific Polymerase Chain Reaction) analysis in 49 fresh...

Diuron, a substituted urea herbicide, is carcinogenic to the urinary bladder of rats at high dietary levels. Its proposed mode action (MOA) includes urothelial cytotoxicity and necrosis followed by regenerative cell proliferation sustained hyperplasia. Cytotoxicity could be induced either solids or chemical toxicity diuron and/or metabolites excreted in urine. Diuron was not genotoxic previous single-cell gel (comet) assay, but possible cross-linking activity remained evaluated. The present...

Atualmente, o método mais importante para a identificação de agentes químicos cancerígenos é teste longa duração in vivo, com roedores. No Brasil não há condições sua execução, devido à inexistência especialistas e infra-estrutura adequada. Em conseqüência, país dependente dos conhecimentos gerados no exterior sobre risco que determinados compostos impõem às populações ao meio ambiente. Por outro lado, as desvantagens inerentes protocolo deste teste, como complexidade na tempo prolongado...

Buccal mucosa cells/Cytokinesis-block micronucleus assay/Head-and-neck cancer/Radiotherapy.This study evaluated cytogenetic damage by measuring the frequency of micronucleated cells (MNC) in peripheral blood and buccal head-and-neck cancer patients undergoing radiotherapy.MNC frequencies were assessed 31 before, during, after radiotherapy, 17 healthy controls matched for gender, age, smoking habits.Results showed no statistically significant difference between prior to radiotherapy...

Diuron (3-(3,4-dichlorophenyl)-1,1-dimethylurea) is a substituted urea herbicide that induces rat urinary bladder urothelial tumors at high dietary levels (2500 ppm). The specific mode of action and molecular alterations triggered by diuron, however, have not been clarified. present study evaluated the dose-dependent effects mucosal transcriptional changes in rats exposed to diuron. Six-week-old male Wistar were treated with 0, 60, 125, 1250, 2500 ppm diuron diet for 20 weeks. Histologic...

The Brazilian federal government Agency for Health Surveillance detected pesticide residues in fresh food available consumers all over the country. current study investigated effects of a mixture some those pesticides (dichlorvos, dicofol, dieldrin, endosulfan, and permethrin) on reproductive system Sprague-Dawley (SD), Wistar (WT), Lewis (LEW) rats. Female rats from each strain were randomized into three experimental groups fed control diet or diets added with at their respective...