A5073521352- Effects of Radiation Exposure
- Blood disorders and treatments
- Radiation Therapy and Dosimetry
- Immunodeficiency and Autoimmune Disorders
- Barrier Structure and Function Studies
- Heat shock proteins research
- Spaceflight effects on biology
- Cancer Cells and Metastasis
- Ultrasound and Hyperthermia Applications
- Neonatal Health and Biochemistry
- Nuclear Physics and Applications
- DNA Repair Mechanisms
- Blood groups and transfusion
- Health, Environment, Cognitive Aging
- Climate Change and Health Impacts
- Trypanosoma species research and implications
- Radioactivity and Radon Measurements
- Connective tissue disorders research
- NF-κB Signaling Pathways
- Anesthesia and Neurotoxicity Research
- Genetics, Aging, and Longevity in Model Organisms
- Research on Leishmaniasis Studies
- Pulmonary Hypertension Research and Treatments
- Radiation Dose and Imaging
- Viral Infections and Immunology Research
University of Pavia
2014-2022
Istituto Nazionale di Fisica Nucleare, Sezione di Pavia
2015
The understanding of the impact radiation quality in early and late responses biological targets to ionizing exposure necessarily grounds on results mechanistic studies starting from physical interactions. This is particularly true when, already at stage, field mixed, as it case for neutron exposure. Neutron Relative Biological Effectiveness (RBE) energy dependent, maximal energies ~1 MeV, varying significantly among different experiments. aim this work shed light effectiveness a function...
Abstract The consideration of how a given technique affects results experimental measurements is must to achieve correct data interpretation. This might be challenging when it comes on biological systems, where unrealistic have full control ( e . g through software replica) all steps in the measurement chain. In this work we address effectiveness different radiation qualities inducing damage can assessed measuring DNA foci yields, only provided that artefacts related scoring are adequately...
Colorectal cancer is one of the most frequent type cancer, with a higher incidence in developed countries. usually managed both surgery, chemotherapy and radiotherapy. Radiotherapy has well-known advantage targeting tumor, minimizing normal tissue exposure. Nevertheless, during radiation treatment, exposure healthy tissues great concern, particular because effects on intestinal barrier functions cells belonging to immune system. The functional role avoiding paracellular trafficking...
Abstract Shwachman‐Diamond syndrome (SDS) is a rare inherited recessive disease mainly caused by mutations in the Shwachman‐Bodian‐Diamond ( SBDS ) gene, which encodes for homonymous protein SBDS, whose function still remains to be fully established. SDS affects several organs causing bone marrow failure, exocrine pancreatic insufficiency, skeletal malformations, and cognitive disorders. About 15% of patients develop myelodysplastic (MDS) are at higher risk developing acute myeloid leukemia...
Shwachman–Diamond syndrome (SDS) (OMIM 260400) is a rare autosomal recessive disease characterized by exocrine pancreatic insufficiency, skeletal, and hematological abnormalities bone marrow (BM) dysfunction. Mutations in the SBDS gene cause SDS. Clonal chromosome anomalies are often present BM, i(7)(q10) del(20q) being most frequent ones. We collected 6 SDS cases with del(20q): cluster of imprinted genes, including L3MBTL1 SGK2 deleted region. Only paternal allele expressed for these genes....
The aim of the present work was to investigate mechanisms radiation-induced bystander signalling leading apoptosis in non-irradiated co-cultured cells. Cultured non-transformed cells were irradiated, and effect on rate malignant determined. For this, two different levels investigation are presented, i.e. release proteins transcriptomic profiling irradiated Concerning proteins, this study, attention focussed active latent forms transforming growth factor-β1 protein. Moreover, global gene...
Journal Article Radiosensitivity in lymphoblastoid cell lines derived from Shwachman–Diamond syndrome patients Get access J. Morini, Morini * 1Department of Molecular Medicine, Biology and Medical Genetics Unit, University Pavia, Italy2Department Physics, Italy3INFN National Institute Nuclear Section Italy *Corresponding author: jacopo.morini01@ateneopv.it Search for other works by this author on: Oxford Academic PubMed Google Scholar G. Babini, Babini 2Department L. Mariotti, Mariotti...
Abstract The inflammatory pathway has a pivotal role in regulating the fate and functions of cells after wide range stimuli, including ionizing radiation. However, molecular mechanisms governing such responses have not been completely elucidated yet. In particular, complex activation dynamics Nuclear transcription Factor kB (NF-kB), key molecule pathway, still lacks complete characterization. this work we focused on NF-kB (subunit p65) following different stimuli. Quantitative measurements...
An approach based on track-structure calculations has been developed to take account of artefacts occurring during γ-H2AX foci detection in 2D images samples analyzed through immunocytochemistry. The need this works stems from the observed saturation yields measured after X-ray doses higher than few grays, hindering an unambiguous quantification DNA damage and radiation effectiveness. proposed modelling allows simulate observer's point view for scoring, mimicking selection a slice Δz cell...
Purpose The aim of this study was to explore the effects chronic low-dose-rate gamma-radiation at a multi-scale level. specific objective obtain an overall view endothelial cell response, by integrating previously published data on different cellular endpoints and highlighting possible mechanisms underpinning radiation-induced senescence. Materials methods Different datasets were collected regarding experiments human umbilical vein cells (HUVECs) which chronically exposed low dose rates (0,...
The protocol adopted in this work aims at unraveling how X-rays perturb the functioning of intestinal barrier, focusing on interplay between colorectal tumor cells and immune system.Colorectal carcinoma is among most common type cancer, typically treated by surgery, chemotherapy, radiotherapy.Advantages radiotherapy targeting are well known.However, even limited exposures healthy tissues great concern, particularly regarding effects barrier system.The setup allows to study two cell...
Ionizing radiation is a peculiar perturbation when it comes to damage biological systems: proceeds through discrete energy depositions, over short temporal scale and spatial critical for subcellular targets as DNA, whose complexity determines the outcome of exposure. This lies at basis success track structure (and nanodosimetry) microdosimetry in biology. However, such reductionist approaches cannot account complex network interactions regulating overall response system radiation,...
Glioblastoma multiforme (GBM) is characterized by a poor prognosis and median survival of ~12-18 months. GBM usually managed neurosurgery followed both chemotherapy radiotherapy. Since develops resistance to conventional therapies, treatment with C-ions promising completely eradicate the tumoural mass. During cranial irradiation, exposure healthy tissues inevitable. Because presence neural stem cells, deep investigation on effects C-ion irradiation respect X-ray induced damage mandatory...
We present predictions of neutron relative biological effectiveness (RBE) for cell irradiations with beams at PTB-Braunschweig. A RBE model is adopted to evaluate initial DNA damage induction given the neutron-induced charged particle field. values are predicted exposures quasi-monoenergetic (0.56 MeV, 1.2 MeV) and a broad energy distribution field dose-averaged 5.75 MeV. Results compared what obtained our neutrons similar energies, when 30-cm sphere irradiated in an isotropic experimental...
The protocol adopted in this work aims at unraveling how X-rays perturb the functioning of intestinal barrier, focusing on interplay between colorectal tumor cells and immune system. Colorectal carcinoma is among most common type cancer, typically treated by surgery, chemotherapy, radiotherapy. Advantages radiotherapy targeting are well known. However, even limited exposures healthy tissues great concern, particularly regarding effects barrier setup allows to study two cell populations a...
Journal Article Investigation of radiation-induced multilayered signalling response the inflammatory pathway Get access G. Babini, Babini * 1Dipartimento di Fisica, Universitá degli Studi Pavia, via Bassi 6, I-27100 Italy2Istituto Nazionale Fisica Nucleare (INFN), Sezione Italy *Corresponding author: gabriele.babini@unipv.it Search for other works by this author on: Oxford Academic PubMed Google Scholar M. Ugolini, Ugolini J. Morini, Morini Italy3Dipartimento Medicina Molecolare, Forlanini...