A5102855110- Corneal Surgery and Treatments
- Wound Healing and Treatments
- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Mesenchymal stem cell research
- Ocular Surface and Contact Lens
- Tendon Structure and Treatment
- Pancreatic function and diabetes
- Diabetic Foot Ulcer Assessment and Management
- Periodontal Regeneration and Treatments
- Muscle Physiology and Disorders
- Corneal surgery and disorders
- Metabolism, Diabetes, and Cancer
- Neuroendocrine Tumor Research Advances
- Immune cells in cancer
- Pressure Ulcer Prevention and Management
- Histiocytic Disorders and Treatments
- Angiogenesis and VEGF in Cancer
- Marine Ecology and Invasive Species
- Oral and Maxillofacial Pathology
- Science, Research, and Medicine
- Nerve injury and regeneration
- Osteoarthritis Treatment and Mechanisms
McGowan Institute for Regenerative Medicine
2019-2025
University of Pittsburgh
2019-2025
Cedars-Sinai Medical Center
2017-2022
Johns Hopkins University
2019
Johns Hopkins Medicine
2019
Ollscoil na Gaillimhe – University of Galway
2013-2018
National University of Ireland
2013
There is a critical clinical need to develop therapies for nonhealing diabetic foot ulcers. Topically applied mesenchymal stromal cells (MSCs) provide novel treatment augment wound healing. A central pathological factor in ulcers an impaired blood supply. It was hypothesized that topically allogeneic MSCs would improve healing by augmenting angiogenesis. Allogeneic nondiabetic bone-marrow derived were seeded collagen scaffold. The full-thickness cutaneous the alloxan-induced rabbit ear ulcer...
Abstract Limbal epithelial stem cells (LESC) maintenance requires communication between and neighboring stromal keratocytes. Extracellular vesicles (EVs) are important for intercellular in various cell niches. We explored the regulatory roles of limbal (LSC)-derived exosomes (Exos), an EV sub-population, (LEC) normal diabetic niche determined differences Exo cargos from LSC. Wound healing proliferation rates primary LEC were significantly enhanced upon treatment by Exos (N-Exos), but not...
Diabetic foot ulceration is the leading cause of amputation in people with diabetes mellitus. Peripheral vascular disease present majority patients diabetic ulcers. Despite standard treatments there exists a high rate. Circulating angiogenic cells previously known as early endothelial progenitor are derived from peripheral blood and support angiogenesis vasculogenesis, providing potential topical treatment for non-healing A scaffold fabricated Type 1 collagen facilitates cell delivery to...
Small non-coding RNAs, in particular microRNAs (miRNAs), regulate fine-tuning of gene expression and can impact a wide range biological processes. However, their roles normal diseased limbal epithelial stem cells (LESC) remain unknown. Using deep sequencing analysis, we investigated miRNA profiles central regions diabetic human corneas. We identified differentially expressed miRNAs limbus vs. cornea (DM) corneas including both type 1 (T1DM/IDDM) 2 (T2DM/NIDDM) diabetes. Some such as miR-10b...
Abstract Functional angiogenesis is a critical therapeutic goal in many pathological conditions. Logically, the use of pro-angiogenic growth factors has been mainstay approach despite obvious limitations and modest success. Recently, macrophages have identified as key regulators host response to implanted materials. Particularly, our understanding dynamically plastic macrophage phenotypes, their interactions with biomaterials, varied roles different stages angiogenic processes evolving...
MiR-146a is upregulated in the stem cell-enriched limbal region vs. central human cornea and can mediate corneal epithelial wound healing. The aim of this study was to identify miR-146a targets primary cells (LECs) using genomic proteomic analyses. RNA-seq combined with quantitative proteomics based on multiplexed isobaric tandem mass tag labeling performed LECs transfected mimic control. Western blot immunostaining were used confirm expression some targeted genes/proteins. A total 251...
Skeletal muscle has a robust, inherent ability to regenerate in response injury from acute chronic. In severe trauma, however, complete regeneration is not possible, resulting permanent loss of skeletal tissue referred as volumetric (VML). There are few consistently reliable therapeutic or surgical options address VML. A major limitation investigation possible therapies the absence well-characterized large animal model. this study, we present results comprehensive transcriptomic, proteomic,...
ABSTRACT Volumetric muscle loss (VML) is an acute trauma that results in persistent inflammation, supplantation of tissue with fibrotic scarring, and decreased function. The cell types, nature cellular communication locations drive the aberrant VML response have remained elusive. Herein, we used spatial transcriptomics integrated single-cell RNA sequencing on mouse canine models administered VML. We observed engenders a unique pro-fibrotic pattern driven by crosstalk between macrophages...
Background: The ability to reinnervate a muscle in the absence of viable nerve stump is challenging clinical scenario. Direct neurotization (DMN) an approach overcome this obstacle; however, success depends on formation new endplates, process, which often limited due lack appropriate axonal pathfinding cues. Objective: This study explored use porcine extracellular matrix hydrogel as neuroinductive interface between and rat DMN model. goal was establish whether such can be used improve...
The precis continuum manifold of cell state macrophages and fibroblast cells during skin fibrosis, described as a chronic inflammatory tissue remodeling microenvironment, is not well understood. Both populations respond directly to damage present coordinated chain activation states that dictates the reparative outcome. spectrum change in phenotype late important, because it by both autocrine paracrine communication contribute deposition extracellular matrix (ECM) inflammation. Here, we...