A5002988736- Zebrafish Biomedical Research Applications
- Environmental Toxicology and Ecotoxicology
- Anesthesia and Sedative Agents
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Anesthesia and Neurotoxicity Research
- Animal Nutrition and Physiology
- Pesticide and Herbicide Environmental Studies
- Pharmaceutical and Antibiotic Environmental Impacts
- Treatment of Major Depression
- Genomics, phytochemicals, and oxidative stress
- Diet and metabolism studies
- Meat and Animal Product Quality
- Aquaculture disease management and microbiota
- Phytochemicals and Antioxidant Activities
- Rabbits: Nutrition, Reproduction, Health
- Aquaculture Nutrition and Growth
- Veterinary Pharmacology and Anesthesia
- Physiological and biochemical adaptations
- Agriculture Sustainability and Environmental Impact
- Pluripotent Stem Cells Research
- Optical Imaging and Spectroscopy Techniques
- Free Radicals and Antioxidants
- Nuts composition and effects
- Carcinogens and Genotoxicity Assessment
- Animal Genetics and Reproduction
University of Trás-os-Montes and Alto Douro
2014-2025
Portuguese League Against Epilepsy
2024
Universidade do Porto
2011-2024
Cooperativa de Ensino Superior Politécnico e Universitário
2023
Association for the Development of Douro Viticulture
2021
Instituto de Biologia Molecular e Celular
2016
Polytechnic Institute of Viseu
2011-2013
Marymount University
2011
Ketamine is often associated with altered mitochondrial function and oxidative stress. Nevertheless, limited data are still available regarding the in vivo action of ketamine bioenergetics redox state. Accumulating evidence supports a role for nitric oxide (NO) as possible modulator ketamine's side effects. In present study, we investigated NO modulation on anesthesia at level brain status.Adult male rats received single dose (50, 100, or 150 mg/kg IP) combination N-nitro-L-arginine (3 IP)....
Glyphosate-based herbicides (GBH) are the most used in world, carrying potentially adverse consequences to environment and non-target species due their massive inadequate use. This study aimed evaluate effects of acute exposure a commercial formulation glyphosate, Roundup® Flex (RF), at environmentally relevant higher concentrations zebrafish larvae through assessment inflammatory, oxidative stress cell death response. Transgenic Tg(mpxGFP)i114 wild-type (WT) (72 h post-fertilisation) were...
Human Papillomavirus (HPV) is the main cause of cervical cancer, and formulations have been widely used to treat vaginal lesions caused by HPV. Herein, liposomes with acridine orange derivative C8 were produced functionalized AT11 aptamer. Subsequently, they incorporated into a formulation, prepared based on universal placebo which included Thymus vulgaris (TEO) or Origanum vulgare (OEO) essential oils. The formulation was technologically characterized permeation tissue determined. To assess...
In the last decade, a considerable number of studies have broadened our knowledge nociceptive mechanisms pain, global health problem in both humans and animals. The use herbal compounds such as eugenol, menthol, thymol, carvacrol analgesic agents has accompanied growing interest this area, offering possible solution for complex problem. Here, we aimed to explore how these natural substances—at three different concentrations (2, 5 10 mg/L)—affect pain responses zebrafish (Danio rerio) larvae...
Background The permutation entropy, the approximate and index of consciousness are some most recently studied electroencephalogram-derived indexes. In this work, a thorough comparison these indexes was performed using propofol anesthesia in rabbit model. Methods Six rabbits were anesthetized with three infusion rates: 70, 100, 130 mg · kg⁻¹ h⁻¹, each maintained for 30 min, random order animal. Data recording awake animals 20, 25, min after rate begun recovered consisted electroencephalogram...
Ketamine is a noncompetitive antagonist of the NMDA-receptors, used as dissociative anesthetic, presently included in category psychoactive substances known "club drugs". administration was associated with impaired working memory and increased psychopathological symptoms, but there lack information regarding effects chronic sub-anesthetic doses. Adult Wistar rats were administered ketamine, 5 10 mg/kg twice daily, subcutaneously for 14 days. One week later, tested an object...
This study aimed to define appropriate humane endpoints (HEs) for an animal model of colorectal carcinogenesis (CRC). Twenty-nine male Wistar rats were divided into two control groups (CTRL1 and CTRL2) injected with ethylenediamine tetraacetic acid (EDTA)–saline solutions induced (CRC1 CRC2) 1,2-dimethylhydrazine (DMH) seven weeks. A score sheet 14 biological parameters was used assess welfare. Groups CRC1 CTRL1 CRC2 CTRL2 euthanized 11 17 weeks after the first DMH administration,...
Azoxystrobin is a broad-spectrum strobilurin fungicide for use on wide range of crops available to end-users as formulated products. Due its extensive application, it has been detected in aquatic ecosystems, raising concerns about environmental impact, which still poorly explored. The objective this work was study the effects commercial formulation azoxystrobin zebrafish embryo model. Sublethal and lethal were monitored during exposure period from 2 h post fertilisation (hpf) 96 hpf after...
Thymol (THY) and 24-epibrassinolide (24-EPI) are two examples of plant-based products with promising therapeutic effects. In this study, we investigated the anti-inflammatory, antioxidant anti-apoptotic effects THY 24-EPI. We used zebrafish (Danio rerio) larvae transgenic line (Tg(mpxGFP)i114) to evaluate recruitment neutrophils as an inflammatory marker site injury after tail fin amputation. another experiment, wild-type AB were exposed a well known pro-inflammatory substance, copper...
Cancers induced by HPV remain a significant public health threat, fueling the study of new therapies.
Brassica by-products are a source of natural bioactive molecules such as glucosinolates and isothiocyanates, with potential applications in the nutraceutical functional food industries. However, effects oral sub-chronic exposure to broccoli by-product flour (BF) have not yet been evaluated. The objective this pilot study was analyse BF intake physiological parameters FVB/N mice fed 6.7% BF-supplemented diet for 21 days. Glucosinolates their derivatives were also quantified plasma urine....
The production of chestnut (Castanea sativa Miller) is mostly concentrated in Europe. Chestnut recognized by its high content antioxidants and phytosterols. This work aimed to evaluate the effects dietary consumption over physiological variables FVB/n mice. Eighteen male 7-month-old mice were randomly divided into three experimental groups (n = 6): 1 (control group) fed a standard diet; 2 diet supplemented with 0.55% (w/w) chestnut; 3 1.1% chestnut. Body weight, water, food intake recorded...
The use of anesthetics has been suggested as a strategy to hamper live fish transport-induced stress. Still, there is insufficient data available on the alternative MS-222. This study investigated propofol mitigate stress in Nile tilapia (Oreochromis niloticus, 143.8 ± 20.9 g and 20.4 0.9 cm) during 6 h simulated transport. Individuals (n = 7) were divided into three groups: control, 40 mg L−1 MS-222, 0.8 propofol. A naïve group non-transported was also considered. During transport 24 after,...