A5084765656- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Gene expression and cancer classification
- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Streptococcal Infections and Treatments
- Astronomy and Astrophysical Research
- Geophysics and Gravity Measurements
- Bacterial Identification and Susceptibility Testing
- vaccines and immunoinformatics approaches
- Plant Pathogens and Fungal Diseases
- Lipoproteins and Cardiovascular Health
- Chromosomal and Genetic Variations
- Oral Health Pathology and Treatment
- Genetic diversity and population structure
- Garlic and Onion Studies
- Genomics and Rare Diseases
- Plant-based Medicinal Research
- Yersinia bacterium, plague, ectoparasites research
- Enterobacteriaceae and Cronobacter Research
- Forensic and Genetic Research
- Methane Hydrates and Related Phenomena
- Agricultural pest management studies
- Protein Kinase Regulation and GTPase Signaling
- Diagnosis and treatment of tuberculosis
Nazarbayev University
2020-2024
L. N. Gumilyov Eurasian National University
2024
National Center for Biotechnology
2018-2021
Cultivated grapevine (Vitis vinifera L. ssp. sativa D.C.) is one of the oldest agricultural crops, each variety comprising an array clones obtained by vegetative propagation from a selected vine grown single seedling. Most within are identical, but some show different form accession, giving rise to new divergent phenotypes. Understanding associations among genotypes crucial efficient management and effective improvement. Inter-primer binding-site (iPBS) markers may aid in determining inside...
Abstract Objectives The data presented in this study were collected with the aim of obtaining complete genomes specific strains Bacillus bacteria, namely, licheniformis T5. This strain was chosen based on its enzymatic activities, particularly amylolytic activity. In study, nanopore sequencing technology employed to obtain genome sequences strain. It is important note that these represent a focused objective within larger research context, which involves exploring biochemical features...
Kazakhstan has a high burden of multidrug-resistant tuberculosis in the Central Asian region. This study aimed to perform genomic characterization Mycobacterium strains obtained from Kazakhstani patients with pre-extensively drug-resistant diagnosed Kazakhstan.Whole-genome sequencing was performed on 10 M. different regions Kazakhstan. All had high-confidence resistance mutations according grading system previously established by World Health Organization. The genome analysis using...
We developed a comprehensive multiplexed set of primers adapted for the Oxford Nanopore Rapid Barcoding library kit that allows universal SARS-CoV-2 genome sequencing. This primer is designed to up any variants pool whole-genome sequencing using single- or double-tiled amplicons from 1.2 4.8 kb with Nanopore. also applicable tasks like targeted proposed here an optimized protocol synthesize cDNA Maxima H Minus Reverse Transcriptase specific primers, which has high yields template RNA and...
Bacteroides fragilis is one of the most important human anaerobic pathogens often found in various clinical infections. The purpose this study was to determine susceptibility a B. strain (BFR_KZ01) from Kazakhstan commonly used anti-anaerobic drugs at local level and detect genes associated with resistance these antibiotics. Species identification bacterial isolate performed by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF/MS) 16S rRNA gene...
Tuberculosis (TB) is an infectious disease that remains essential public health problem in many countries. Despite decreasing numbers of new cases worldwide, the incidence antibiotic-resistant forms (multidrug resistant and extensively drug-resistant) TB increasing. Next-generation sequencing technologies provide a high-throughput approach to identify known novel potential genetic variants are associated with drug resistance Mycobacterium tuberculosis (Mtb). There limited reports data...
High-throughput sequencing platforms generate a massive amount of high-dimensional genomic datasets that are available for analysis. Modern and user-friendly bioinformatics tools analysis interpretation genomics data becomes essential during the data. Different standard types file formats have been developed to store analyze sequence Variant Call Format (VCF) is most widespread type format containing information variants sequenced samples.Existing processing VCF files don't usually an...
Drug-resistant tuberculosis (TB) is a major public health problem. Clinical Mycobacterium (MTB) isolate with Extensively drug-resistant (MTB-XDR) profile was subjected to whole-genome sequencing using next-generation platform (NGS) Roche 454 GS FLX+ followed by bioinformatics sequence analysis. Quality of read checked FastQC, paired-end reads were trimmed Trimmomatic. De novo genome assembly conducted Velvet v.1.2.10. The assembled XDR-TB-1599 strain functionally annotated the PATRIC...
ABSTRACT Fusobacterium varium is a Gram-negative, invasive, obligate anaerobe in the gastrointestinal tract microbiota, associated with various clinical conditions, including colorectal cancer (CRC). Here, we announce draft genome sequence of two F. strains Fv36kaz and Fv63kaz from patients CRC Kazakhstan.
The horse (Equus caballus) is a domesticated animal with great significance in human civilization and history, having played crucial role transportation, agriculture, warfare. Over millennia, intentional breeding has resulted the creation of approximately 500 distinct breeds, each selected for specific performance qualities, appearance, behavior (Petersen et al., 2013). earliest evidence domestication dates back to Eneolithic Botai culture (3500 BCE) prehistoric Northern Kazakhstan, where...
Familial hypercholesterolemia is an autosomal hereditary disease defined by increased level of low-density lipoprotein cholesterol (LDL-C), which predisposes significant risks for premature cardiovascular disorders. We present a family trio study: proband, 13-year-old Kazakh girl with homozygous familial (HoFH) and her parents. HoFH much more rare severe than heterozygous form the disorder. patients generally LDL-C levels exceeding 13 mmol/L, resulting in early life-threatening events within...
Fusobacterium nucleatum is an invasive obligate anaerobe found in the oral microbiota and associated with colorectal cancer. Here, we announce draft genome sequence of strain Fn11kaz from a patient cancer Kazakhstan.
Independent Component Analysis is a matrix factorization method for data dimension reduction. ICA has been widely applied the analysis of transcriptomic blind separation biological, environmental, and technical factors affecting gene expression. The study aimed to analyze publicly available esophageal cancer using identification comprehensive reproducible signaling pathways molecular signatures involved in this type. In study, four independent datasets from GEO databases were used. A...
Kazakhstan is a Central Asian crossroad of European and populations situated along the way Great Silk Way. The territory has historically been inhabited by nomadic tribes today multi-ethnic country with dominant Kazakh ethnic group. We sequenced analyzed whole-genomes five healthy individuals high coverage using next-generation sequencing platform. This whole-genome sequence data can be valuable reference for biomedical studies investigating disease associations population-wide genomic...
Abstract Objectives: The data presented in this study were collected with the aim of obtaining complete genomes specific strains Bacillus bacteria, namely, licheniformis T5. This strain was chosen based on its enzymatic activities, particularly amylolytic activity. In study, nanopore sequencing technology employed to obtain genome sequences strain. It is important note that these represent a focused objective within larger research context, which involves exploring biochemical features...
In Kazakhstan, the total incidence rate of tuberculosis in 2021 was 74 cases per 100,000 population. The aim this study characterization drug-resistant (DR) <i>M. tuberculosis</i> (MTB) collected Kazakhstan and to perform pilot whole genome sequencing for drug resistance detection. Drug susceptibility testing first/second-line anti-TB drugs done by using BACTEC MGIT-960 system 144 clinical isolates. Pilot (WGS) performed MiSeq (Illumina) platform 22 isolates tuberculosis</i>. Data analysis...