A5056529326- Salmonella and Campylobacter epidemiology
- Animal Nutrition and Physiology
- Viral gastroenteritis research and epidemiology
- Listeria monocytogenes in Food Safety
- Probiotics and Fermented Foods
- Gut microbiota and health
- Food Safety and Hygiene
- Pharmaceutical and Antibiotic Environmental Impacts
- Essential Oils and Antimicrobial Activity
- Vibrio bacteria research studies
- Escherichia coli research studies
- Bacteriophages and microbial interactions
- Bacterial biofilms and quorum sensing
- Livestock and Poultry Management
- Identification and Quantification in Food
- Aquaculture disease management and microbiota
- Microbial infections and disease research
- Clostridium difficile and Clostridium perfringens research
- Antibiotic Resistance in Bacteria
- Antibiotics Pharmacokinetics and Efficacy
- Protein Hydrolysis and Bioactive Peptides
- Trace Elements in Health
- Plant Virus Research Studies
- Antimicrobial Resistance in Staphylococcus
- Genomics and Phylogenetic Studies
University of Tennessee at Knoxville
2011-2019
University of Arkansas at Fayetteville
2008-2012
Tecnológico de Monterrey
2012
Fayetteville State University
2011
Data indicate that prevalence of specific serovars Salmonella enterica in human foodborne illness is not correlated with their feed. Given feed a suboptimal environment for S. , it appears survival poultry may be an independent factor unrelated to virulence . Additionally, appear have different host specificity and the ability cause disease those hosts also serovar dependent. These differences among related gene presence or absence expression levels genes. With better understanding...
The objective of this study was to determine if survival culturable Campylobacter jejuni outside the host increased by entrapment in pre-established biofilms.Campylobacter inoculated into four biofilm populations isolated from poultry environments and cultured at three temperatures. Survival Camp. some biofilms extended vs broth. But were detrimental jejuni. Denaturing gradient gel electrophoresis analysis indicated differences bacterial profiles depending on initial source temperature...
Journal Article Salmonella enterica isolates from pasture‐raised poultry exhibit antimicrobial resistance and class I integrons Get access S.N. Melendez, Melendez The Department of Food Science, University Arkansas, Fayetteville, AR, USA Search for other works by this author on: Oxford Academic Google Scholar I. Hanning, Hanning Irene B. Science Department, Division Agriculture, 2650 North Young Avenue, AR 72704‐5690, USA. E‐mail: ihanning@uark.edu J. Han, Han U.S. Drug Administration,...
Three pathogens, Campylobacter, Salmonella, and Shiga-toxin-producing Escherichia coli, are leading causes of bacterial gastroenteritis in the United States worldwide. Although these three bacteria typically considered food-borne outbreaks have been reported due to contaminated drinking water irrigation water. The aim this research was develop two types PCR assays that could detect quantify Campylobacter spp., E. coli O157:H7, Salmonella watershed samples. In conventional PCR, target strains...
Much work has been dedicated to identifying members of the microbial gut community that have potential augment growth rate agricultural animals including chickens. Here, we assessed any correlations between fecal microbiome, a proxy for and feed efficiency or weight gain at pedigree chicken level, highest tier production process. Because selective breeding is conducted our aim was determine if microbiome profiles could be used predict conversion in order improve breeding. Using 16s rRNA...
Abstract: The growing interest in organic and natural foods warrants a greater need for information on the food safety of these products. In this study, samples were taken from 2 pasture flock farms ( N = 178; feed, water, drag swabs, insect traps), retail carcasses 48) 1 processing facility 16) over period 8 mo. A total 105 Campylobacter isolates obtained 53 (30%), 36 (75%), 16 (100%) farms, carcasses, facility, respectively. Of collected, 65 C. jejuni, 31 coli , 9 other spp. Using PCR,...
Feed components have low water activity, making bacterial survival difficult. The mechanisms of Salmonella in feed and subsequent colonization poultry are unknown. purpose this research was to compare the ability serovars strains survive broiler evaluate molecular associated with by measuring expression genes (hilA, invA) via fatty acid synthesis (cfa, fabA, fabB, fabD). inoculated 1 15 enterica consisting 11 (Typhimurium, Enteriditis, Kentucky, Seftenburg, Heidelberg, Mbandanka, Newport,...
Salmonella is a leading cause of foodborne illness in the United States, with poultry and products being primary source infection to humans. Poultry may carry some serovars without any signs or symptoms disease causing adverse effects health bird. be introduced flock by multiple environmental sources, but feed suspected source. Detecting can challenging because low levels bacteria not recovered using traditional culturing techniques. Numerous detection methodologies have been examined over...
Listeria monocytogenes is a ubiquitous, saprophytic, Gram-positive bacterium and occasional food-borne pathogen, often associated with ready-to-eat meat products. Because of the increased consumer interest in organic, all natural, free range poultry products, it important to understand L. context such systems. Pasture-reared were surveyed over course two 8-wk rearing periods. Cecal, soil, grass samples collected for isolation characterization. Seven 399 cecal (or 1.75%) Listeria-positive....
Campylobacter jejuni is a leading cause of foodborne illness, with poultry and products being sources infection. Epidemiological efforts to trace can be challenging because the extreme genetic diversity this bacterium relative other pathogens. To enhance tracking epidemiological efforts, whole-genome sequencing has been used for pathogens but not yet evaluated practicality Campylobacter. Thus, purpose study was evaluate as genotyping method C. by comparing it 2 commonly methods, namely...
When prebiotics and other fermentation substrates are delivered to animals as feed supplements, the typical goal is improve weight gain conversion. In this work, we examined pasture flock chicken cecal contents using next generation sequencing (NGS) identify understand composition of microbiome when were supplemented. We generated 16S rRNA data for 120 separate samples from groups chickens receiving one 3 or fiber additives. The indicated that respective additives enrich specific bacterial...
The objectives of this work were to evaluate immunomagnetic beads and a reverse transcriptase (RT)-PCR method for the detection Salmonella inoculated into feed. In addition, was evaluated quantifying virulence gene hilA expression ssp. in poultry feed matrices utilized determine influence environmental factors on expression.An separation technique increased recovery from cultures samples exposed heat treatments 70°C sampled periodically. From these samples, RNA collected measured relative...