OBJECTIVE. Extremely low gestational age neonates have very oxygen saturation in utero and an immature antioxidant defense system. Abrupt increases after birth may cause oxidative stress. We compared achievement of a targeted 85% at 10 minutes life when resuscitation was initiated with or high fractions inspired levels were adjusted according to preductal pulse values. METHODS. A prospective, randomized, clinical trial performed 2 level III neonatal referral units. Patients ≤28 weeks...

Mycophenolate mofetil (MMF) is a widely prescribed immunosuppressive agent after solid organ transplantation. Potential teratogenic effects in utero exposure to MMF experimental studies and clinical observations humans has been postulated recent literature. However, specific pattern of malformation not identified yet. We present newborn patient, born recipient renal transplantation, who became pregnant while taking as therapy. The exhibited cleft lip palate, bilateral microtia atretic...

Plum pox virus (PPV) is able to replicate in inoculated leaves of Nicotiana tabacum, but defective systemic movement this host. However, PPV produces a infection transgenic tobacco expressing the silencing suppressor P1/HC-Pro from etch (TEV). In work we show that move upper non-inoculated plants bacterial salicylate hydroxylase (NahG) degrades salicylic acid (SA). Replication and accumulation higher locally infected deficient SA or TEV suppressor. Accumulation viral derived small RNAs was...

Antenatal steroids have improved the survival of preterm infants; however, mechanism action is not fully understood. We aimed to establish an association between antenatal and antioxidant activity postnatal oxidative stress. In a prospective cohort study, extremely neonates receiving (CORT) or (NOCORT) were enrolled. An activities enzymes glutathione cycle in cord blood was found. addition, reduced stress (GSH/GSSG ratio, CORT vs. NOCORT, 35.68 ± 12.20 28.38 9.92; p < 0.01) and, decreased...

Abstract Purpose: Previous gene expression studies have shown that fibroblast growth factor receptor 3 (FGFR3) is overexpressed in early stages of bladder cancer. To study the potential use therapeutic antibodies against FGFR3, we produced a collection human single-chain Fv (scFv) antibody fragments by using phage display libraries. Experimental Design: Two “naïve” semi-synthetic scFv libraries were used to select extracellular domain FGFR3α(IIIc). The reactivity selected scFvs with...

Abstract Purpose: Fibroblast growth factor receptor 3 (FGFR3) mutations have been associated with achondroplastic syndromes and urinary bladder carcinomas. Here we describe changes in FGFR3 mRNA protein expression transitional carcinomas determine the effect of monoclonal antibodies against RT-112 cell line proliferation. Experimental Design: We used microarray tools to evaluate 22 at different stages (noninvasive pTa, lamina propria invasive pT1, muscular pT2) 7 nonneoplastic tissue...

A full-length genomic cDNA clone of a plum pox potyvirus (PPV) isolate belonging to the M strain (PPV-PS) has been cloned downstream from bacteriophage T7 polymerase promoter and sequenced. Transcripts resulting plasmid, pGPPVPS, were infectious and, in herbaceous hosts, produced symptoms that differed those virus progeny pGPPV, D PPV-R. Viable PPV-R/-PS chimeric viruses constructed by recombination clones vitro. Analysis plants infected with different chimeras indicated sequences encoding...

ABSTRACT Plum pox virus (PPV) is a member of the Potyvirus genus that, in nature, infects trees Prunus genus. Although PPV systemically several species Nicotiana genus, such as N. clevelandii and benthamiana , replicates inoculated leaves tabacum it unable to infect last host. The long-distance movement defect was corrected transgenic tobacco plants expressing 5"-terminal region genome etch (TEV), potyvirus that tobacco. fact move upper noninoculated transformed with same TEV transgene, but...

Plum pox virus (PPV) is a member of the genus Potyvirus that able to infect large variety plant species, including trees Prunus, its natural host. When some PPV isolates are propagated for an extended time in herbaceous plants, their ability reduced. The molecular basis this change host infectivity poorly understood. We report construction hybrid viruses from cDNA clones two D-strain PPV, PPV-D and PPV-R, which differ range. can GF305 peach seedlings efficiently, however, it unable Nicotiana...

The goal of the study was to compare preductal SpO2 in first 10 min after birth preterm infants treated with non-invasive continuous positive airway pressure (CPAP) and air a published nomogram who received no medical intervention, examine gender differences.Prospective observational study.We enrolled ≤32 weeks gestation were spontaneously breathing heart rate >100 bpm, face mask CPAP during postnatal stabilisation. limits targeted at ≥75% 5 ≥85% bpm. FIO2 titrated against SpO2. Preductal...

RNA silencing has a well-established function as an antiviral defence mechanism in plants and insects. Using Agrobacterium-mediated transient assay, we report here that NS1 protein from human influenza A virus suppresses manner similar to P1/HC-Pro of Tobacco etch potyvirus, well-characterized plant suppressor. Moreover, have shown expression strongly enhances the symptoms Potato X three different hosts, suggesting could be inhibiting mechanisms activated on infection. These data provide...

SUMMARY The potyviruses Plum pox virus (PPV) and Tobacco vein mottling (TVMV) have distinct host ranges induce different symptoms in their common herbaceous hosts. To test the relevance of P1 protein compatibility pathogenicity, hybrid viruses were constructed which coding sequence PPV was completely or partially replaced by corresponding sequences from TVMV. Infections induced these chimeric revealed that TVMV a PPV/TVMV proteins are functionally equivalent plants to isolate adapted hosts,...

SUMMARY Subisolates segregated from an M‐type Plum pox virus (PPV) isolate, PPV‐PS, differ widely in pathogenicity despite their high degree of sequence similarity. A single amino acid substitution, K109E, the helper component proteinase (HCPro) protein PPV caused a significant enhancement symptom severity herbaceous hosts, and notably modified infectivity peach seedlings. The presence this substitution certain subisolates that induced mild symptoms hosts did not infect seedlings suggested...

<h3>Background:</h3> Psychological stress of parents preterm infants is aggravated by prolonged hospitalisation. Early discharge programmes (EDPs) have been implemented to alleviate this situation. <h3>Objective:</h3> To evaluate parental psychological in an EDP for the first 3 months after neonatal intensive care unit (NICU) discharge. <h3>Design/methods:</h3> Prospective randomised trial comparing assigned (n = 72) or standard programme (SDP) (standard discharge) 68). At discharge, were...

&lt;b&gt;&lt;i&gt;Background:&lt;/i&gt;&lt;/b&gt; The therapeutic decision to close patent ductus arteriosus in preterm infants entails great uncertainty. Near-infrared spectroscopy is a noninvasive bedside technique used monitor mixed regional oxygen saturation. We hypothesized that near-infrared could identify at risk of mesenteric hypoperfusion associated with hemodynamically significant arteriosus. &lt;b&gt;&lt;i&gt;Methods:&lt;/i&gt;&lt;/b&gt; This prospective observational study...

Several subisolates were separated from a single Plum pox virus (PPV) isolate, PPV-PS. In spite of an extremely high sequence conservation (more than 99.9% similarity), different differed largely in pathogenicity herbaceous hosts and infectivity woody plants. The severity symptomatology did not seem to correlate with accumulation. Sequence analysis site-directed mutagenesis demonstrated that amino acid changes the helper component (HC) protein caused drastic effect on symptoms notably...

Caffeine citrate is the treatment of choice for apnea prematurity (AOP). Regulatory agencies have requested real-world data on drug utilization and safety, a postauthorization safety study, pharmaceutical-grade caffeine citrate, Peyona, to confirm its benefit preterm infants.To investigate clinical use, outcomes, profile this in routine infants with gestational age (GA) <37 weeks.We conducted multicenter, noninterventional, prospective study five European countries. Patients eligible...

ABSTRACT The characterization of pathogenic properties two infectious clones Plum pox virus (PPV) isolates, pGPPV (D group) and pGPPVPS (M group), was investigated in their woody hosts (seedlings Prunus spp.). differed ability to infect plum peach cultivars, from no infection local systemic infection. phenotype determinants were located with a set chimeric viruses the clones. In plum, genomic fragment encoding P3 6K1 proteins, which might influence genome amplification or movement. capacity...