A5069172989- CRISPR and Genetic Engineering
- PI3K/AKT/mTOR signaling in cancer
- Genetics, Aging, and Longevity in Model Organisms
- Pluripotent Stem Cells Research
- Single-cell and spatial transcriptomics
- Biochemical Analysis and Sensing Techniques
- Renal and related cancers
- Genomics, phytochemicals, and oxidative stress
- Fungal and yeast genetics research
- Hearing, Cochlea, Tinnitus, Genetics
- Calcium signaling and nucleotide metabolism
- Protein Kinase Regulation and GTPase Signaling
- Invertebrate Immune Response Mechanisms
- Cellular transport and secretion
- Neurobiology and Insect Physiology Research
- Vestibular and auditory disorders
- Autophagy in Disease and Therapy
Max Planck Institute for Biology of Ageing
2022-2024
University of Washington
2017-2019
Seattle University
2017
Abstract Amino acid (AA) availability is a robust determinant of cell growth through controlling mechanistic/mammalian target rapamycin complex 1 (mTORC1) activity. According to the predominant model in field, AA sufficiency drives recruitment and activation mTORC1 on lysosomal surface by heterodimeric Rag GTPases, from where it coordinates majority cellular processes. Importantly, however, teleonomy proposed regulation acts its effector proteins remain enigmatic. Here, using multiple...
To reveal how cells exit human pluripotency, we designed a CRISPR-Cas9 screen exploiting the metabolic and epigenetic differences between naïve primed pluripotent cells. We identify tumor suppressor, Folliculin(FLCN) as critical gene required for from pluripotency. Here show that FLCN Knock-out (KO) hESCs maintain state but cannot since transcription factor TFE3 remains active in nucleus. targets up-regulated KO assay are members of Wnt pathway ESRRB. Treatment hESC with inhibitor, not...
Abstract Rapamycin is a macrolide antibiotic that functions as an immunosuppressive and anti‐cancer agent, displays robust anti‐ageing effects in multiple organisms including humans. Importantly, rapamycin analogues (rapalogs) are of clinical importance against certain cancer types neurodevelopmental diseases. Although widely perceived allosteric inhibitor mTOR (mechanistic target rapamycin), the master regulator cellular organismal physiology, its specificity has not been thoroughly...
Abstract Intermittent fasting and fasting-refeeding regimens can slow biological aging across taxa 1 . Shifts between fed fasted states activate ancient nutrient-sensing pathways which alter cellular epigenetic to promote longevity 2–4 Yet how age trajectories progress during fasting-refeeding, reprogram state remain largely unknown. Here we observe increases in predicted of Caenorhabditis elegans prolonged adult reproductive diapause, followed by extraordinary reduction refeeding. We...
Aging stem cells lose the capacity to properly respond injury and regenerate their residing tissues. Here, we utilized ability of Drosophila melanogaster germline (GSCs) survive exposure low doses ionizing radiation (IR) as a model adult cell identified regeneration defect in aging GSCs: while GSCs IR, they fail reenter cycle timely manner. Mechanistically, identify foxo mTOR homologue, Tor important regulators GSC quiescence following radiation. is required for entry quiescence, essential...
To easily edit the genome of naïve human embryonic stem cells (hESC), we introduced a dual cassette encoding an inducible Cas9 into AAVS1 site hESC (iCas9). The iCas9 line retained karyotypic stability, expression pluripotency markers, differentiation potential, and stability in 5iLA EPS conditions. induced efficient homology–directed repair (HDR) non-homologous end joining (NHEJ) based mutations through CRISPR-Cas9 system. We utilized to study epigenetic regulator, PRC2 early pluripotency....
Abstract Amino acid (AA) availability is a robust determinant of cell growth, through controlling mTORC1 activity 1 . According to the predominant model in field, AA sufficiency drives recruitment and activation on lysosomal surface by heterodimeric Rag GTPases, from where it coordinates majority cellular processes (reviewed 2,3 ). Importantly, however, 15 years after its initial discovery, teleonomy proposed regulation mTORC1, acts effector proteins remain enigmatic 4 Here, using multiple...
Abstract Rapamycin is a macrolide antibiotic that functions as an immunosuppressive and anti-cancer agent, displays robust anti-ageing effects in multiple organisms including humans. Importantly, rapamycin analogs (rapalogs) are of clinical importance against certain cancer types neurodevelopmental diseases. Although widely perceived allosteric inhibitor mTOR (mechanistic target rapamycin), the master regulator cellular organismal physiology, its specificity has not been thoroughly evaluated...