A5018527380- Neuroscience and Neuropharmacology Research
- Physics of Superconductivity and Magnetism
- Cell Adhesion Molecules Research
- Neurogenesis and neuroplasticity mechanisms
- Advanced Fluorescence Microscopy Techniques
- Theoretical and Computational Physics
- Luminescence Properties of Advanced Materials
- Advanced Condensed Matter Physics
- Protease and Inhibitor Mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- 3D Printing in Biomedical Research
- Neurobiology and Insect Physiology Research
- Cellular Mechanics and Interactions
- Advanced Photocatalysis Techniques
- Click Chemistry and Applications
- Biosensors and Analytical Detection
- Insect and Arachnid Ecology and Behavior
- Retinal Development and Disorders
- Genetic Syndromes and Imprinting
- Copper-based nanomaterials and applications
- Advanced biosensing and bioanalysis techniques
- Genetics and Neurodevelopmental Disorders
- Autism Spectrum Disorder Research
- Ammonia Synthesis and Nitrogen Reduction
- Protein Kinase Regulation and GTPase Signaling
The University of Tokyo
2007-2021
International Superconductivity Technology Center
1992-2018
Tokyo Medical and Dental University
2009-2016
Japan Science and Technology Agency
2015
IHI Corporation (Czechia)
2013
University of Washington
2001-2011
NEC (Japan)
2009
Dalhousie University
2009
Osaka City University
2001-2007
Harborview Medical Center
2002
Insulin activates the ras proto-oncogene product p21ras (Ras) by stimulating conversion of inactive GDP-bound form Ras to active GTP-bound form. The protein ASH (for abundant Src homology) (Matuoka, K., Shibata, M., Yamakawa, A., and Takenawa, T. (1992) Proc. Natl. Acad. Sci. U. S. A. 89, 9015-9019) is composed one homology (SH)2 two SH3 domains highly homologous Caenorhabditis elegans sem-5 that couples a tyrosine kinase protein. We have studied an interaction with insulin-stimulated...
Abstract The Escherichia coli lac Z gene encoding β‐galactosidase is a widely used reporter, but few synthetic substrates are available for detecting its activity with single‐cell resolution in living samples. Our recently reported fluorogenic substrate SPiDER‐βGal suitable this purpose, hydrolysis product shows green fluorescence emission, and red‐shifted analogue therefore required use combination fluorescent protein (GFP) markers. Herein, we describe the development of β‐galactosidase,...
Abstract Neuroligin 3 (NLGN3) and neurexins (NRXNs) constitute a canonical transsynaptic cell-adhesion pair, which has been implicated in autism. In autism spectrum disorder (ASD) development of sociality can be impaired. However, the molecular mechanism underlying NLGN3-mediated social is unclear. Here, we identify non-canonical interactions between NLGN3 protein tyrosine phosphatase δ (PTPδ) splice variants, competing with NRXN binding. NLGN3-PTPδ complex structure revealed...
Abstract —Proliferation and α v β 3 integrin–dependent migration of vascular smooth muscle cells are suppressed on polymerized type I collagen. To identify genes specifically regulated in human by collagen, we used the suppressive subtraction hybridization technique. Compared with cultured monomer collagen suppresses following: (1) a number other extracellular matrix proteins, including fibronectin, thrombospondin-1, tenascin-C, cysteine-rich protein 61; (2) actin binding proteins α-actinin;...
Abstract Centrosome number is associated with the chromosome segregation and genomic stability. The ubiquitin–proteasome system considered to be main regulator of centrosome number. However, here we show that autophagy also regulates centrosomes. Autophagy-deficient cells carry extra autophagic regulation dependent on a centrosomal protein 63 (Cep63) given lacking contain multiple Cep63 dots are engulfed digested by in wild-type cells, upregulation increases recruited autophagosomes via...
The local structure of superconducting ${\mathrm{YBa}}_{2}$${\mathrm{Cu}}_{4}$${\mathrm{O}}_{8}$ is studied by atomic pair-distribution analysis pulsed-neutron-scattering data. Collective displacements chain oxygen atoms forming polarized microdomains are found at low temperatures, but the domains disappear above spin-gap temperature. size smaller than 20 \AA{}. These observations suggest an active role lattice in charge localization and formation spin gap.
Highlights•BMP4 is transported in dense-core vesicles along the axon•BMP4 release triggered by activity induces its perisynaptic clustering•Surface BMP4 clusters destabilize nearby synapses through type I receptors•BMP4 expression levels presynaptic neurons critically regulates synapse stabilitySummaryIn vitro screening of signaling molecules involved neural circuit formation has identified a large number synaptogenic proteins. However, factors that drive elimination remain elusive. Here, we...
A fast, high-sensitivity photothermal microscope was developed by implementing a spatially segmented balanced detection scheme into laser scanning microscope.We confirmed 4.9 times improvement in signal-to-noise ratio the compared with that of conventional detection.The system demonstrated simultaneous bi-modal and confocal fluorescence imaging transgenic mouse brain tissue pixel dwell time 20 μs.The image visualized neurons expressing yellow proteins, while signal detected endogenous...
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Cell transplantation after enclosing in microcapsules has been studied as an alternative approach for treatment of wide variety diseases. In the present study, we examined feasibility using agarose microcapsules, having a cell-enclosing hollow core 100–150 μm diameter and gel membrane about 20 thickness, device methodology. We enclosed cells that had genetically engineered to express cytochrome P450 2B1, enzyme activates anticancer prodrug ifosfamide. The were shown enzymatic function they...
Vascular gene therapy could potentially complement or replace current therapies for human atherosclerosis, while avoiding their side effects. However, development of vascular is limited by lack a useful vector. Helper-dependent adenovirus (HDAd) shows promise to overcome this barrier because, unlike first-generation adenovirus, HDAd achieves durable transgene expression in the artery wall with minimal inflammation. To begin test whether HDAd, delivered wall, can limit atherosclerosis we...
Abstract Maternally inherited duplication of chromosome 15q11-q13 (Dup15q) is a pathogenic copy number variation (CNV) associated with autism spectrum disorder (ASD). Recently, paternally derived has also been shown to contribute the development ASD. The molecular mechanism underlying paternal Dup15q remains unclear. Here, we conduct genetic and overexpression-based screening identify Necdin ( Ndn ) as driver gene for resulting in ASD-like phenotypes mice. An excess amount results enhanced...
Cylindromatosis tumor suppressor protein (CYLD) was initially identified as a deubiquitylating in familial cylindromatosis patients. Proteomic analyses using rodent brain samples revealed enrichment of CYLD purified postsynaptic density fractions. Here, we report that regulates dendritic growth and differentiation mouse hippocampal neurons. showed diffuse localization rapidly growing dendrites, but gradually concentrated spines. Overexpression knockdown the early stage cultured neurons...
Objective— Vascular smooth muscle cells (SMCs) cultured on polymerized type I collagen fibrils are arrested in the G 1 phase of cell cycle, and their phenotypic markers pattern expressed genes markedly altered. In this study, we examined regulation plasminogen activator inhibitor (PAI)-1 its involvement SMC migration. Methods Results— We demonstrate that secretion surface accumulation PAI-1 suppressed SMCs compared with monomer collagen. replated vitronectin after culture result at focal...
The roles of calcium-calmodulin-dependent protein kinase II-alpha (CaMKIIα) in the expression long-term synaptic plasticity adult brain have been extensively studied. However, how increased CaMKIIα activity controls maturation neuronal circuits remains incompletely understood. Herein, we show that pyramidal neurons without upregulate rate spine addition, resulting elevated density. Genetic elimination specifically eliminated observed maturation-dependent suppression formation. Enhanced...
Microglia regulate synapse stability and remodeling through multiple molecular pathways. Regulated spatial distribution of microglia within nervous tissues may affect dynamics. Here, we focused on the relationship between spine synapses in mouse neocortex found that distance microglial cell bodies (MCBs) spines is a critical parameter stability. The region close to MCBs contains processes with higher density more contacts. This also shows extensive exploration tissue space by processes. To...
Abstract The Escherichia coli lac Z gene encoding β‐galactosidase is a widely used reporter, but few synthetic substrates are available for detecting its activity with single‐cell resolution in living samples. Our recently reported fluorogenic substrate SPiDER‐βGal suitable this purpose, hydrolysis product shows green fluorescence emission, and red‐shifted analogue therefore required use combination fluorescent protein (GFP) markers. Herein, we describe the development of β‐galactosidase,...
We provide evidence for a genetic link between the adrenocortical X zone and agouti (a) locus on chromosome 2 in segregating inbred mouse strain SM/J. This has been established maintained while keeping heterozygosity (Aw/a) at is, therefore, of advantage assessing effects these alleles upon physiological phenomena. In Aw/a (white-bellied agouti) or a/a (black) virgins, zones were composed exclusively nonvacuolated cells, they contained vacuolated cells only Aw/Aw. suggested that might...