A5084726701- Insect symbiosis and bacterial influences
- Insect Resistance and Genetics
- Chromosomal and Genetic Variations
- Genomics and Phylogenetic Studies
- Entomopathogenic Microorganisms in Pest Control
- Mosquito-borne diseases and control
- Insect-Plant Interactions and Control
- Genetic diversity and population structure
- Plant and animal studies
- Plant Virus Research Studies
- Studies on Chitinases and Chitosanases
- Malaria Research and Control
- Ecology and Vegetation Dynamics Studies
- CRISPR and Genetic Engineering
- Pacific and Southeast Asian Studies
- Insect behavior and control techniques
- Genetic Mapping and Diversity in Plants and Animals
- Insect Pest Control Strategies
- Insect and Arachnid Ecology and Behavior
- Plant Disease Resistance and Genetics
- Invertebrate Immune Response Mechanisms
- Genetic and Environmental Crop Studies
- Animal Ecology and Behavior Studies
- Nematode management and characterization studies
Centre National de la Recherche Scientifique
2006-2025
Université Claude Bernard Lyon 1
2005-2025
Laboratoire de Biométrie et Biologie Evolutive
2012-2025
VetAgro Sup
2015-2024
Institut national de recherche en informatique et en automatique
2015
Université de Montpellier
2011
Imperial College London
2009
Repetitive DNA, including transposable elements (TEs), is found throughout eukaryotic genomes. Annotating and assembling the "repeatome" during genome-wide analysis often poses a challenge. To address this problem, we present dnaPipeTE-a new bioinformatics pipeline that uses sample of raw genomic reads. It produces precise estimates repeated DNA content TE consensus sequences, as well relative ages families. We shows dnaPipeTE performs using very low coverage sequencing in different genomes,...
Abstract Inferring the history of isolation and gene flow during species differentiation can inform us on processes underlying their formation. Following recent expansion in Europe, two subspecies house mouse ( Mus musculus domesticus ) have formed a hybrid zone maintained by incompatibilities possibly behavioural reinforcement, offering good model incipient speciation. We reconstruct divergence using an approximate Bayesian computation framework sequence variation at 57 autosomal loci. find...
The rice weevil Sitophilus oryzae is one of the most important agricultural pests, causing extensive damage to cereal in fields and stored grains. S. has an intracellular symbiotic relationship (endosymbiosis) with Gram-negative bacterium Sodalis pierantonius a valuable model decipher host-symbiont molecular interactions.We sequenced genome using combination short long reads produce best assembly for Curculionidae species date. We show that undergone successive bursts transposable element...
Transposable elements (TEs) are ubiquitous and mobile repeated sequences. They major determinants of host fitness. Here, we characterized the TE content spotted wing fly Drosophila suzukii. Using a recently improved genome assembly, reconstructed sequences de novo found that TEs occupy 47% mostly located in gene-poor regions. The majority insertions segregate at low frequencies, indicating recent probably ongoing activity. To explore dynamics context biological invasions, studied variation...
Transposable elements (TEs) are the main components of genomes. However, due to their repetitive nature, they very difficult study using data obtained with short-read sequencing technologies. Here, we describe an efficient pipeline accurately recover TE insertion (TEI) sites and sequences from long reads by Oxford Nanopore Technology (ONT) sequencing. With this pipeline, could precisely landscapes most recent TEIs in wild-type strains
Abstract Transposable elements (TEs) play a pivotal role in genome evolution, yet their detection and annotation remain challenging due to the limitations of current methods. Manual curation is considered gold standard for generating TE libraries, particularly TE-focused studies, although it requires extensive training time. With rapid increase number assembly publications growing need large-scale comparative analyses, automated software has become indispensable. This study compares manual...
In Drosophila, like in many other animal species, females tend to live longer than males, a phenomenon known as sex gap longevity (SGL). One of the possible causes underlying this could be related high content transposable elements (TE) Y chromosome (toxic hypothesis). TE activity is normally repressed by epigenetic mechanisms, but regulation weakens with age. Since rich TEs, age-related should more pronounced old males females, likely affecting patterns. work, we studied natural variation...
Invasive species represent unique opportunities to evaluate the role of local adaptation during colonization new environments. Among these species, Asian tiger mosquito, Aedes albopictus, is a threatening vector several human viral diseases, including dengue and chikungunya, raises concerns about Zika fever. Its broad presence in both temperate tropical environments has been considered reflection great "ecological plasticity." However, no study conducted assess adaptive evolution ecological...
Some species of parasitic wasps have domesticated viral machineries to deliver immunosuppressive factors their hosts. Up now, all described cases fall into the Ichneumonoidea superfamily, which only represents around 10% hymenoptera diversity, raising question whether such domestication occurred outside this clade. Furthermore, biology ancestral donor viruses is completely unknown. Since 1980s, we know that Drosophila parasitoids belonging Leptopilina genus, diverged from superfamily 225 Ma,...
Genome size varies considerably among organisms due to differences in the amplification, deletion, and divergence of various kinds repetitive sequences, including transposable elements, which constitute a large fraction genome. However, while changes genome observed at wide taxonomic level have been thoroughly investigated, we still know little about process involved closely related species. We estimated sizes reverse transcriptase–related sequence (RTRS) content nine species Drosophila...
Abstract The two species of the palm genus Howea (Arecaceae) from Lord Howe Island, a minute volcanic island in Tasman Sea, are now regarded as one most compelling examples sympatric speciation, although this view is still disputed by some authors. Population genetic and ecological data necessary to provide more coherent comprehensive understanding emerging model system. Here, we analyse on abundance, juvenile recruitment, pollination mode variation structure both species. We find that...
The insertion polymorphism of five transposable element (TE) families was studied by Southern blots in several populations the M and S molecular forms mosquito Anopheles gambiae sensu stricto from southern Cameroon. We showed that mean TE site number within-population globally differed between forms. comparison profiles revealed a significant differentiation these two (0.163 < Phi(ST) 0.371). cloned insertions non-LTR retrotransposon (Aara8) were fixed one form absent other one. only could...
Abstract During biological invasion process, species encounter new environments and partially escape some ecological constraints they faced in their native range, while face ones. The Asian tiger mosquito Aedes albopictus is one of the most iconic invasive introduced every inhabited continent due to international trade. It has also been shown be infected by a prevalent yet disregarded microbial entomoparasite Ascogregarina taiwanensis. In this study, we aimed at deciphering factors that...
Abstract Transposable elements are mobile repeated sequences found in all genomes. controlled by RNA interference pathways most organisms, and this control involves the PIWI-interacting pathway small interfering pathway, which is also known to be first line of antiviral defense invertebrates. Using Drosophila, we recently showed that viral infections result modulation transposable element transcript levels through repertoire. The Aedes aegypti mosquito particular interest because almost half...
Abstract Transposable Elements (TEs) are ubiquitous and mobile repeated sequences. They major determinants of host fitness. Here, we portrayed the TE content spotted wing fly Drosophila suzukii . Using a recently improved genome assembly, reconstructed sequences de novo , found that TEs occupy 47% mostly located in gene poor regions. The majority insertions segregate at low frequencies, indicating recent probably ongoing activity. To explore dynamics context biological invasions, studied...
Abstract Background The rice weevil Sitophilus oryzae is one of the most important agricultural pests, causing extensive damage to cereal in fields and stored grains. S. has an intracellular symbiotic relationship (endosymbiosis) with Gram-negative bacterium Sodalis pierantonius a valuable model decipher host-symbiont molecular interactions. Results We sequenced genome using combination short long reads produce best assembly for Curculionidae species date. show that undergone successive...
To circumvent host immune response, some hymenopteran endo-parasitoids produce virus-like structures in their reproductive apparatus that are injected into the together with eggs. These viral-like absolutely necessary for reproduction of these wasps. The viral evolutionary origin particles has been demonstrated only a few cases wasp species all belonging to Ichneumonoidea superfamily. In addition, nature initial virus-wasp association remains unknown all. This is either because no closely...
Abstract Invasive species represent unique opportunities to evaluate the role of local adaptation during colonization new environments. Among these species, Asian tiger mosquito, Aedes albopictus , is a threatening vector several human viral diseases, including dengue and chikungunya, raises concerns about Zika fever. Its broad presence in both temperate tropical environments has been considered reflection great “ecological plasticity”. However, no study conducted assess adaptive evolution...