A5110995653- Liver physiology and pathology
- Parasites and Host Interactions
- Mesenchymal stem cell research
- MicroRNA in disease regulation
- SARS-CoV-2 and COVID-19 Research
- Parasite Biology and Host Interactions
- Cancer, Hypoxia, and Metabolism
- Organ Transplantation Techniques and Outcomes
- Parasitic Diseases Research and Treatment
- SARS-CoV-2 detection and testing
- Circular RNAs in diseases
- Liver Disease Diagnosis and Treatment
- Pancreatic function and diabetes
- Advanced biosensing and bioanalysis techniques
- Nanoparticles: synthesis and applications
- Biosensors and Analytical Detection
- Chemotherapy-induced cardiotoxicity and mitigation
- Curcumin's Biomedical Applications
- Therapeutic Uses of Natural Elements
- Complement system in diseases
- Helminth infection and control
- Aldose Reductase and Taurine
- Moringa oleifera research and applications
- Advances in Cucurbitaceae Research
- Electrospun Nanofibers in Biomedical Applications
Theodor Bilharz Research Institute
2013-2025
National Research Centre
2022
Cairo University
2022
Zagazig University
2020
Doxorubicin (DOX) is an anti-neoplastic therapy, but its use limited by deleterious toxic effects including nephrotoxicity and cardiotoxicity. This work aimed at assessing the potential protective effect of Ceratonia siliqua methanol extract (CME) on DOX-induced in 5 groups Wistar rats. Nephrotoxicity was induced experimentally intraperitoneal (IP) injection DOX (15 mg/kg). increased serum creatinine, urea, sodium, potassium levels. It elevated MDA levels renal tissue decreased concentration...
Abstract Background The freshwater snails Biomphalaria alexandrina and Bulinus trancatus are key contributors to the transmission of S. mansoni haematobium , respectively, for being their intermediate hosts. Objectives This research study aimed investigate potency nucleoproteins (NPs) extracted from both snail species on host immune reactions as an approach developing a potential vaccine. Methods Three groups six-week-old Swiss-Webster mice ( n = 18; 15–20 g each) were injected...
Recently, nanotherapy has been considered a promising and rapidly evolving field to combat cancer. Both gold nanoparticles chitosan are interesting materials with biological antitumor properties. The present study aimed synthesise chitosan/gold nanocomposites (Cs/Au) using gamma irradiation technique their anticancer, antibiofilm antimicrobial activities. synthesized Cs/Au were characterized various analytical tools such as UV-vis, XRD, TEM. UV-vis absorption spectra exhibited surface...
Abstract Background Defining the protective thresholds against severe-acute-respiratory-syndrome-related corona virus-2 pandemic is a crucial challenge. To reduce risks of severe disease, hospitalization, and death, various COVID-19 vaccines have been rapidly developed. Aim work This study aimed to assess impact three common vaccine types; two mRNA vaccines: (Pfizer/BioNTech’s BNT162b2 Moderna’s mRNA-1273), one adenoviral vector vaccine: Oxford/AstraZeneca’s ChAdOx1, inactivated (Sinovac...
The aim of the present research is to elucidate anti-oxidant and anti-tumor activities mucin extracted from Ereminia desertorum snails´ mucus against two types tumor cell lines; human colon adenocarcinoma (CACO-2) cells hepatoma (HepG-2) cells.Both lines were treated with oxidative markers measured in culture media by biochemical gene expression analysis using RT-PCR. suppressor was also evaluated RT-PCR.The HepG-2 or CACO-2 extract have high significant increased levels catalase, SOD, GSH...
Diabetes is usually associated with oxidative stress that causes hepatic and pancreatic tissue injury. This work was carried out to evaluate the effect of Cucumis sativus Cucurbita maxima methanol extracts on streptozotocin-induced diabetic injury in rats. induced seven equal groups rats by a single intraperitoneal injection streptozotocin (40 mg/kg), addition non-diabetic control group. Two were treated extract two maxima, each at 200 400 mg/kg for 21 days after diabetes. Another group both...
Despite the transfer of COVID-19 from pandemic to control, we are still in a state uncertainty about long-term success. Therefore, there is great need for rapid and sensitive diagnostics sustain control status. After several optimization trials, developed lateral flow test (LFT) strips detection SARS-CoV-2 spike 1 (S1) antigen saliva samples. For signal enhancement our strips, applied dual gold conjugates. Gold-labeled anti-S1 nanobodies (Nbs) were employed as S1 detector conjugate, while...
Mesenchymal stem cell (MSC)-based liver tissue engineering on nanofibrous scaffold holds great promise for cell-based therapy in injuries and end-stage failure treatments. MSCs were generated from umbilical cord blood. Hepatogenic differentiation was induced two-dimensional (2D) three-dimensional (3D) culture system characterized by morphology, scanning electron microscopy, immunocytochemistry, gene expression. Albumin α-1 antitrypsin (AAT) supernatants measured. Differentiated cells...
Background. The liver is one of the major target organs for which cell-based therapies are very promising. limitations various cellular therapies, including bone marrow (BM)-derived mesenchymal stem cells (MSCs), urges exploration cell sources more suitable transplantation. Human umbilical cord blood (HUCB) can overcome these drawbacks with a favorable reparative outcome.Objectives. aim this study was to evaluate therapeutic potential MSCs in 2 groups chronic injury experimental...
Objective: To develop a new sandwich based lateral flow immunochromatographic strip for rapid detection of circulating Schistosoma mansoni antigen in serum and urine samples patients with active schistosomiasis.Methods: This was prepared by using anti-Schistosoma soluble egg monoclonal antibody conjugated gold nanoparticles (MAb-AuNPs) as antigen-detecting antibody, while crystalline material (MCM)-41-MAb bioconjugate immobilized at the test line antigen-capturing antibody. Both capturing...
Abstract Background Hepatocellular carcinoma (HCC) is the leading cause of death worldwide. Nanoparticles allow early detection tumor and delivery chemotherapeutic drugs to specific site. This study aimed assess therapeutic role dumbbell-like nanoparticles conjugated with monoclonal antibodies (mAbs) against both vascular endothelial growth factor (VEGF) cluster differentiation (CD) 90 (a cancer stem cell marker) in hepatocellular experimental model. included 100 mice; HCC was induced...
Abstract Compared to other infectious diseases, for which LFT development can take years, SARS-CoV-2 antigen LFTS were developed and deployed within months. detection adopted on an unprecedented scale during the COVID-19 pandemic, but many of them lack sensitivity especially samples with low viral load. In our previous work, we enhanced signal strip SARS CoV-2 SI antigens in saliva. Here introduce some modification improve sensitivity, specificity, lower cost strip, by using biotin...
Liver transplant is the cornerstone line of treatment for chronic liver diseases; however, long list complications and obstacles stand against this operation. Searching new modalities illness a must. In present research, we aimed to compare effects undifferentiated human mesenchymal stem cells, in vitro differentiated adult hepatocytes an experimental model failure.Undifferentiated cord blood cells were isolated, pro-pagated, characterized by morphology, gene expression analysis, flow...
Abstract Objective To evaluate the cytotoxic activity and cytopathological changes of Vitex agnus-castus L. (V. agnus-castus) leaves extracts characterize their bioactive chemical constituents. Methods The dried powder V. was extracted using 85% methanol (MeOH). methanolic extract defatted petroleum ether fractionated ethyl acetate (EtOAc) butanol (BuOH). anticancer potential different evaluated by neutral red assay, apoptosis caspase-3 expression in hepatoma cell line (HepG2). constituents...
Orthotopic liver transplantation is an established treatment for patients with severe acute and end-stage chronic disease.The shortage of donor organs continues to be the rate-limiting factor throughout world.Hepatocyte a promising several diseases can, also, used as 'bridge' in cases failure.
<b><i>Introduction:</i></b> Malignant ascites results from imbalance between protein in the peritoneal cavity and absorption of fluids via lymphatic system. More than 20 interleukins (ILs) are known to play an important role protection against tumors. <b><i>Materials Methods:</i></b> Ascitic fluid IL-1B, IL-2, IL-4, IL-6, IL-10, TNF-α, IFN-γ levels were assessed 45 patients with liver cirrhosis as judged by histopathological ultrasonographic...
Hepatocellular carcinoma is one of the major health problems throughout world with a very poor prognosis. MicroRNAs are small regulatory non-protein-coding RNA molecules. We aimed at investigating microRNA-199 as potential therapeutic tool for HCC both in vitro and an experimental model. A strategy based on effect microRNAs to target genes responsible liver cancer was adopted this work. The ability these RNAs potently influence cellular behavior also investigated. role miR-199a development...
Liver transplantation is the well-known treatment for chronic liver diseases; however, postoperative complications and lack of donors continue to be limitations with this treatment. Investigating new modalities illness a must. In present study, we aimed clarify effects an in vitro hepatocyte-differentiated human unrestricted somatic stem cell transplant as cell-based therapy experimental model failure.Human umbilical cord blood-derived cells were isolated, cultured, propagated,...
Objectiveto assess the production of Anti-Schistosoma Monoclonal Antibodies by growing IgM producing hybridoma cell line in serum low medium and to evaluate their diagnostic potential replace old conventional method animal ascetic fluid.MethodsHighly reactive specific MAb secreting S. mansoni SEA was cultured three different media; rich replacement (conventional), (SLRM) free (SFRM).ResultsCell count viability were better SLRM than SFRM. The lower detection limit assay Mab culture 1.1 ng/ml...