Methicillin resistant

Abstract Background The freshwater snails Biomphalaria alexandrina and Bulinus trancatus are key contributors to the transmission of S. mansoni haematobium , respectively, for being their intermediate hosts. Objectives This research study aimed investigate potency nucleoproteins (NPs) extracted from both snail species on host immune reactions as an approach developing a potential vaccine. Methods Three groups six-week-old Swiss-Webster mice ( n = 18; 15–20 g each) were injected...

Pharmacognosy Research,2018,10,4,368-378.DOI:10.4103/pr.pr_89_18Published:October 2018Type:Original ArticleAuthors:Mosad Ghareeb, Amal Saad, Wafaa Ahmed, Laila Refahy, and Sami Nasr Author(s) affiliations:Mosad Ghareeb1, Saad1, Ahmed2, Refahy1, Nasr3 1Department of Medicinal Chemistry, Theodor Bilharz Research Institute, Giza, EGYPT. 2Department Egypt; Department College Science Arts, Sajir, Shaqra University, Shaqra, KSA. 3Department Biochemistry Molecular Biology, Abstract:Background:...

Non-alcoholic fatty liver disease (NAFLD) constitutes a major health problem worldwide and intimately links with obesity diabetes. This study aimed to explore the therapeutic impact of early treatment metformin (MTF) alone or in combination Lactobacillus reuteri DSM 17938 (L. reuteri) + metronidazole (MTZ) male Sprague Dawley rats high-fat diet (HFD)-induced NAFLD. Hepatic steatosis was induced by feeding HFD for 6 weeks. MTF (150 mg/kg/day) L. (2 × 109 colony forming unit/day) were given...

Liver cancer stem cells (LCSCs) are a subpopulation of tumor that can drive initiation and relapses. Because their significance, researchers looking for biomarkers characterize or regulate LCSCs so they be used as targets the diagnosis treatment chronic liver diseases hepatocellular carcinoma (HCC).Six groups patients having hepatitis C virus (HCV), HCV + cirrhosis, HCC, B (HBV), HBV in addition to control group, were subjected measurement levels analysis miR-1290 miR-1825 expression.The...

Chemical constituents of Argyreia speciosa Fam. Convolvulaceae and its role against hyperglycemiaAli S. A, Hamed M.A, El- Rigal N.S, Shabana M.H, Kassem M.E.S.

This work aims at studying Biomphalaria alexandrina snails as a source of bioactive compounds. Adult were maintained in plastic, mud and cement matrices. Three groups prepared; under normal laboratory conditions, slightly low pH high temperature (30°C) conditions. Nine fungal species isolated from soft tissues snails. Ethyl acetate acetone extracts prepared each species, their biological activities investigated. Paecilomyces variotii the most effective antimicrobial agents, while extract...

Sulfadiazine is a drug famous for its anti-inflammatory, antimicrobial, and antitumor effects. Remarkably, biological activity can be further enhanced upon incorporating suitable metal ion. However, these metal-comprising complexes are not widely available. In the current study, sulfadiazine Schiff base derivative was exploited as ligand synthesizing new utilizing Cu(II), Co(II), Ni(II), Fe(III), Cr(III) metals. The structural analytical characteristics of newly prepared were elucidated...

Pharmacognosy Research,2018,10,2,179-180.DOI:10.4103/pr.pr_145_17Published:April 2018Type:Original ArticleAuthors:Sami M. Nasr, Mosad A. Ghareeb, Mona Mohamed, Nehal Elwan, Abd El-Wanes Anter Abdel-Aziz, and Mohamed S. Abdel-Aziz Author(s) affiliations:Sami M Nasr1, A Ghareeb2, Mohamed2, Elwan3, Abdel-Aziz2, S Abdel-Aziz4 1Biochemistry Molecular Biology Department, Theodor Bilharz Research Institute, Kornaish El-Nile, 12411 Warrak El-Hadar, Imbaba (P.O. 30), EGYPT. 2Medicinal Chemistry Egypt...

Background: Cecropin-B (Cec-B) is an Antimicrobial Peptide (AMP) found in insects. Objectives: Recombinant production of Cec-B peptide Escherichia coli (Rosetta™ DE3), and studying its anticancer effect on hepatocellular carcinoma cell line (HCC). Methods: The gene Drosophila melanogaster was synthesized by PCR assembly using the simplified synthesis (SGS) method. To express recombinant E. DE3); cloned into pET-15b expression vector. expressed as insoluble aggregates called inclusion bodies...

Streptokinase (SK) is a potent plasminogen activator naturally produced by beta-hemolytic streptococcus bacteria and used as thrombolytic drug. Optimize high yield production of recombinant streptokinase (rSK) in Escherichia coli evaluate its activity. Synthetic gene encoding mature SK protein with optimization for rare codons mRNA secondary structure was cloned into the expression vector pET-3a transformed BL21 (DE3). Seed banks were established rSK clones. The native optimized using IPTG...

Fasciolosis is one of the neglected tropical diseases that negatively affect farming industry and public health. The present study aimed to evaluate a polymerase chain reaction (PCR) assay for detection infection with Fasciola sp. in field-collected Lymnaea natalensis snails from two rural localities Egypt; compare between it ordinary microscopic methods. A total 1000 lymnaeid were collected, then samples examined by crushing, cercarial shedding molecular average sensitivity methods compared...

A new steroidal saponin, named as (25R)-6α-[(β-D-glucopyranosyl) oxy]-5α-spirostan-3β-yl β-D-glucopyranosyl (1''→3')-O-β-D-glucopyranosid (2) was isolated from the flowers of Cestrum elegans.In addition, a known saponin (1), flavonoid compound (3) along with triterpene (4) were for first time in genus and identified based on their chromatographic properties, chemical spectral data (HR-ESI-MS, 1 H, 13 C NMR, H-1 H COSY, HSQC, HMBC).The four compounds evaluated antibacterial, antifungal...

Herpes simplex virus -2 (HSV-II) increases the prevalence of life-threatening viral infections so; there is a great need to find and develop new compounds combat them. This study aims compare chemical composition, antioxidant potential cell-mediated blocking HSV-II activities Alpinia officinarum (AO) Zingiber officinale (ZO) root extracts. Phenolic flavonoid contents were determined; was evaluated by DPPH• (2,2-diphenyl-1-picryl-hydrazyl-hydrate) as well investigation antiviral activity. The...