A5061631416- Parasites and Host Interactions
- Helminth infection and control
- Parasite Biology and Host Interactions
- Drug-Induced Hepatotoxicity and Protection
- Research on Leishmaniasis Studies
- Liver Disease Diagnosis and Treatment
- Drug Transport and Resistance Mechanisms
- Trypanosoma species research and implications
- Hepatitis C virus research
- Child Nutrition and Water Access
- Trace Elements in Health
- Phytochemistry and biological activities of Ficus species
- Pharmacological Effects and Toxicity Studies
- Phytochemistry and Bioactive Compounds
- Diet and metabolism studies
- Hepatitis B Virus Studies
- Liver physiology and pathology
- Glutathione Transferases and Polymorphisms
- Pharmacogenetics and Drug Metabolism
- Gut microbiota and health
- Phytochemistry and Bioactivity Studies
- Biotechnology and Related Fields
- Global Maternal and Child Health
- Nigella sativa pharmacological applications
- Body Image and Dysmorphia Studies
Theodor Bilharz Research Institute
2014-2025
Birmingham Women's Hospital
2012-2013
Ministry of Health and Population
2005
World Health Organization
2005
Ain Shams University
1990
Institut de Biologie et de Chimie des Protéines
1987-1988
Recent evidence suggest that resistance to praziquantel (PZQ) may be developing. This would not surprising in countries like Egypt where the drug has been used aggressively for more 10 years. The classic phenotype of is a significant increase 50% effective dose value isolates retrieved from patients responding drug. In previous publication, we reported such phenotypes have isolated humans infected with Schistosoma mansoni. Since action PZQ dependent upon and host factors, most notably immune...
Results from infected patients, not cured by multiple doses of praziquantel (PZQ), have been reported different geographic locations, suggesting that resistance to the drug may be present. This has coupled with several in vivo (e.g., studies on mice 'resistant isolates') and vitro tests direct application measurement effects schistosomes maintained culture) demonstrating a significant reduction drug's efficacy. Despite little field evidence are becoming less sensitive drug, 100% cure after...
Cyclophosphamide (CP) is a potent anti-neoplastic and immunosuppressive agent; however, it causes multi-organ toxicity. We elucidated the protective activities of Eucalyptus globulus (EG) leaf extract against CP-induced hepato-renal Mice were treated with EG for 15 days plus CP on day 12 13 experiment. Using HPLC-DAD-ESI-MS/MS, 26 secondary metabolites identified in extract. Out them, 4 polyphenolic compounds isolated: (1) 4-(O-β-d-xylopyranosyloxy)-3,5-di-hydroxy-benzoic acid, (2)...
The aggressive use of praziquantel to combat schistosomiasis in Egpyt raises concern about the possible emergence resistance. Eggs from Egyptian patients with praziquantel-resistant infections (not cured by 3 doses praziquantel) have been used establish infection-specific schistosome isolates mice. response these worms drug was observed vitro, order determine if obtained resistant were, fact, less responsive praziquantel. One hallmark effects on schistosomes vitro is a disruption worm's...
Schistosomiasis, a high volume neglected tropical disease affecting more than 200 million people worldwide, can only be effectively treated by the tetrahydroisoquinoline drug praziquantel (PZQ). Herein, we describe an efficient approach to access PZQ derivatives Ugi 4-component reaction followed Pictet-Spengler in two-step, one-pot procedure. 30 novel are described based on and X-ray structure of derivative revealing different conformation compared with is discussed. Several analogues...
Abstract Schistosomiasis drastically affects human health, where S. mansoni -induced hepatic fibrosis remains a serious problem with no available drug yet. The current study aimed to evaluate the hepatoprotective effects of Vildagliptin (Vilda), Diaminodiphenyl Sulfone (DDS), and their combination (Vilda/DDS) against elucidate underlying molecular mechanisms. S.mansoni -infected mice were administered praziquantel (PZQ) for two consecutive days, or Vilda, DDS, Vilda/DDS 14 days....
A pool of 38 pan-African Centres Excellence (CoEs) in health innovation has been selected and recognized by the African Network for Drugs Diagnostics Innovation (ANDI), through a competitive criteria based process. The process identified number opportunities challenges R&D continent: i) it provides direct evidence existence capability that can be leveraged to fill specific gaps continent; ii) revealed research financing pattern is largely fragmented uncoordinated, iii) highlights most...
Few studies reported the antifibrotic effects of gallic acid (GA) despite its known hepatoprotective and antioxidant activities. Accordingly, this study investigated GA through clarifying mechanisms on hepatic stellate cells' (HSCs) activation, proliferation and/or apoptosis. In vitro HSC-T6 activation/proliferation, morphology safety hepatocytes were assessed. vivo, fibrosis was induced via chronic thioacetamide (TAA)-intoxication. TAA-intoxicated rats treated with silyamrin or GA. At end...
Hibiscus sabdariffa L. (Malvaceae) is a common traditional tea that has many biological activities.To evaluate the hepatoprotective effect and study metabolic profile of anthocyanin-rich extract H. calyces (HSARE).The activity HSARE was assessed (100 mg/kg/d for 4 weeks) by examining hepatic, inflammatory, oxidative stress markers performing histopathological examination in rats with thioacetamide (TAA)-induced hepatotoxicity. analyzed using ultra-performance liquid...
Schistosomiasis is responsible for a considerable global disease burden. This work aimed to improve the therapeutic outcome of only available antischistosomal drug worldwide, praziquantel (PZQ), by incorporating it into novel carrier, "solid lipid nanoparticles (SLNs)", enhance its solubility, bioavailability and efficacy. A simple, cost-effective method was used prepare SLN-PZQ. Compared market PZQ (M-PZQ), SLN-PZQ more bioavailable, as denoted higher serum concentrations in both normal...
Background Fascioliasis is an emerging zoonotic disease of considerable veterinary and public health importance. Triclabendazole the only available drug for treatment. Laboratory studies have documented promising fasciocidal properties artemisinins (e.g., artemether). Methodology We carried out two exploratory phase-2 trials to assess efficacy safety oral artemether administered at (i) 6×80 mg over 3 consecutive days, (ii) 3×200 within 24 h in 36 Fasciola-infected individuals Egypt. Efficacy...
In a multicenter investigation of the potential antischistosomal activity myrrh, resin obtained from an African plant, different derivatives resin, including commercial preparation Mirazid, were tested at doses in mice and hamsters infected with Schistosoma mansoni. Egyptian (CD) strain S. mansoni, four six groups treated Mirazid did not show significant worm reduction, while remaining showed but trivial reductions. Puerto Rican (Mill Hill) solution was toxic for high produced modest or no...
This trial investigated the anti-schistosomal activity of mirazid in comparison with that praziquantel Schistosoma mansoni-infected Egyptian patients. The sample population was composed 1,131 individuals (459 school children and 672 household members). Screening for S. mansoni conducted using standard Kato Katz technique. Four slides from a single stool were examined before treatment, four per samples obtained on three consecutive days post-treatment. All positive eligible subjects randomly...
Resveratrol is a naturally occurring polyphenol, possesses several pharmacological activities including anticancer, antioxidant, antidiabetic, antinociceptive, and antiasthmatic activity. Little known about its hepatoprotective action mechanisms. This study was conceived to explore the possible protective mechanisms of resveratrol compared with silymarin in thioacetamide (TAA)-induced hepatic injury rats. Thirty-two rats were equally divided into four groups; normal control (i), TAA (100...
Non-alcoholic fatty liver disease (NAFLD) constitutes a major health problem worldwide and intimately links with obesity diabetes. This study aimed to explore the therapeutic impact of early treatment metformin (MTF) alone or in combination Lactobacillus reuteri DSM 17938 (L. reuteri) + metronidazole (MTZ) male Sprague Dawley rats high-fat diet (HFD)-induced NAFLD. Hepatic steatosis was induced by feeding HFD for 6 weeks. MTF (150 mg/kg/day) L. (2 × 109 colony forming unit/day) were given...
The activity of the acyclic nucleotide analogue 9-(S)-[3-hydroxy-2-(phosphonomethoxy)propyl]adenine [(S)-HPMPA] against Schistosoma mansoni was investigated in mice. compound injected intraperitoneally, usually on two or five consecutive days, at 10 to 20 mg/kg body weight/day. treatment started before, time of, and after onset egg laying (oviposition) by S. mansoni. animals were killed from 7 40 days cessation treatment. Significant reductions total numbers female coupled worms found....