Methicillin resistant

Non-alcoholic fatty liver disease (NAFLD) constitutes a major health problem worldwide and intimately links with obesity diabetes. This study aimed to explore the therapeutic impact of early treatment metformin (MTF) alone or in combination Lactobacillus reuteri DSM 17938 (L. reuteri) + metronidazole (MTZ) male Sprague Dawley rats high-fat diet (HFD)-induced NAFLD. Hepatic steatosis was induced by feeding HFD for 6 weeks. MTF (150 mg/kg/day) L. (2 × 109 colony forming unit/day) were given...

Context: Linum usitatissimum (Linn); known as Flaxseed, is one of the most important medicinal plants traditionally used for health benefits and also nutritional purposes. Objective: Estimation total phenolic flavonoid contents, evaluation antioxidant, antiinflammatory anticancer activities have been performed on hexane, ethyl acetate, n-butanol, methanol extracts fractions extract (hexane, n-butanol). Materials Methods: Phenolic contents were detected using spectrophotometric colorimetric...

Abstract Inflammation is the response of body's immune system to harmful stimuli. The expression phosphodiesterase 4 enzyme (PDE4) was demonstrated in many inflammatory cells. Thus, it considered an attractive therapeutic target for combating disorders. In present study, a novel class quinazolin‐2,4‐dione analogues 1‐17a,b synthesized. Structures newly synthesized compounds were characterized by means spectral and elemental analysis. Additionally, molecular docking studies new synthetic...

Human MxA gene is related to the class of interferon (IFN)-stimulated genes (ISGs) that plays a role in antiviral resistance.Implementation standard curves obtained from designing procedure for data processing relative qPCR between expression and interferon's activity (IU/ml). These can be used detect any new compound rapidly safely.To optimum incubation time maximum human peripheral blood mononuclear cells (PBMC), isolated PBMCs (1x106 cells) were incubated with concentration 1000 IU/ml...

IFN-β (Rebif) is a drug with valuable cancer therapeutic potential as it mediates anti-proliferation and apoptosis induction. Therefore, this study aimed to evaluate the of through assessing gene expression p53 caspase-3 in hepatocellular carcinoma cell (HCC) line (HepG2). The 50% inhibition viability concentration (IC50) was calculated by cytotoxicity assay, tested on normal (Vero). After treating HepG2 cells IFN-β, analyzed, at time intervals 2, 4, 6, 24 hrs, real-time PCR, histopathology...

An amino acid short chain is known as a peptide. Peptide bonds are the connections that hold acids of peptide together in particular order. Characteristically, shorter length peptides helps to identify them from proteins. Different ways used classify peptides, including length, source or their biological functions. The fact serve several purposes suggests there foundation for improvement production and structure enhance action. In addition, many patents on therapeutic diagnostic approaches...

Abstract Egypt has witnessed the emergence of multidrug-resistant (MDR) Klebsiella pneumoniae , which posed a serious healthcare challenge. The proper treatment choice for MDR-KP infections is not well determined renders problem more complicated, thus making control such challenge professionals. This study aims to encapsulate cationic antimicrobial peptide; Cecropin-B (Cec-B), increase its lifetime, drug targeting, and efficacy effect free encapsulated recombinant rCec-B peptide on K....

Peptide-based therapy has emerged as a promising avenue for treating various disorders, and recent research highlighted the potential of anti-cancer peptides (ACPs) in cancer treatment. In this context, study aimed to design novel peptide incorporating tumor-homing (RGD) C-amidation enhance its anticancer activity, particularly against liver (HepG2) colon (HCT-116) cell lines. The primary objective was with improved properties by leveraging capabilities RGD enhancing activity through...

Background: Cecropin-B (Cec-B) is an Antimicrobial Peptide (AMP) found in insects. Objectives: Recombinant production of Cec-B peptide Escherichia coli (Rosetta™ DE3), and studying its anticancer effect on hepatocellular carcinoma cell line (HCC). Methods: The gene Drosophila melanogaster was synthesized by PCR assembly using the simplified synthesis (SGS) method. To express recombinant E. DE3); cloned into pET-15b expression vector. expressed as insoluble aggregates called inclusion bodies...

Streptokinase (SK) is a potent plasminogen activator naturally produced by beta-hemolytic streptococcus bacteria and used as thrombolytic drug. Optimize high yield production of recombinant streptokinase (rSK) in Escherichia coli evaluate its activity. Synthetic gene encoding mature SK protein with optimization for rare codons mRNA secondary structure was cloned into the expression vector pET-3a transformed BL21 (DE3). Seed banks were established rSK clones. The native optimized using IPTG...

This study compares the immunohistochemical reaction of a new experimental tricalcium silicate perforation repair material to mineral trioxide aggregate (MTA) and Biodentine. A total 162 mature premolar teeth from 12 dogs were divided into three groups (n = 54 each) according evaluation period: 1, 2 3 months. Each group was further two equal subgroups 27 time repair: immediate delayed repair. subgroup subdivided used subdivisions 8 each): MTA, Biodentine (Septodont) material, control...

Fish is a good indicator for monitoring of heavy metals risks. The present study was conducted to evaluate the effects Chlorella vulgaris and Spirulina platensis dietary supplementation on Oreochromis niloticus growth under normal conditions. Additionally, evaluation their performance, growth-related genes expression antioxidant enzymes O. exposed mixture metals. results showed that highest performance recorded in groups supplemented with 10 15% S. compared C. control groups. ghrelin, leptin...

Hepatocellular carcinoma (HCC) is a poor-prognosis type of cancer with high resistance to chemotherapy, making the search for safe drugs mandatory issue. Plant-derived products have potential reduce negative side effects treatments. In this work, ability defatted methanolic extract Alocasia gigantea leaves fight HCC was evaluated in an animal model. Overall, treatment HCC-induced mice at 150 mg/kg body weight four consecutive weeks caused induction autophagy through silencing relative...

The current study aims to evaluate the antioxidant, anticancer and antiviral activities of Phyllanthus emblica leaves, as well characterization bioactive chemical constituents.The total phenolic flavonoid contents plant extracts were determined using Folin-Ciocalteu's aluminium trichloride assays, respectively.The antioxidant evaluated by different methods, activity was via MTT assay, while cytopathic effect (CPE) inhibition anti-HCV assays.Moreover, profiling performed reversed phase...