A5011671437- Colorectal Cancer Surgical Treatments
- Hepatocellular Carcinoma Treatment and Prognosis
- Gastric Cancer Management and Outcomes
- Liver Disease Diagnosis and Treatment
- Mycotoxins in Agriculture and Food
- Colorectal Cancer Screening and Detection
- Gastrointestinal Tumor Research and Treatment
- Colorectal Cancer Treatments and Studies
- DNA Repair Mechanisms
- Pancreatic and Hepatic Oncology Research
- Carcinogens and Genotoxicity Assessment
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Glycosylation and Glycoproteins Research
- Advanced Proteomics Techniques and Applications
- Gastrointestinal disorders and treatments
- Diverticular Disease and Complications
- Mass Spectrometry Techniques and Applications
- Plant Pathogens and Fungal Diseases
- Monoclonal and Polyclonal Antibodies Research
- Diet and metabolism studies
- Colorectal and Anal Carcinomas
- Liver Disease and Transplantation
- Nutrition and Health in Aging
- Metastasis and carcinoma case studies
- Insect Resistance and Genetics
Nagasaki Ajisai Hospital
2018-2024
Gifu Prefectural Tajimi Hospital
2017-2021
Mie University
1979-2019
Heisei College of Health Sciences
2019
Protein Research Foundation
2015-2018
University of Tsukuba
2018
University of Shimane
2018
St. Luke's International Hospital
2018
St. Luke's International University
2018
Ono Pharmaceutical (Japan)
2007-2017
Toward the creation of a robust and efficient producer L-arginine L-citrulline (arginine/citrulline), we have performed reengineering Corynebacterium glutamicum strain by using genetic information three classical producers. Sequence analysis their arg operons identified point mutations (argR123, argG92(up), argG45) in one mutation (argB26 or argB31) each other two Reconstitution former argB on wild-type genome led to no production. Combined introduction argB26 argB31 with argR123 into wild...
Periodic paralysis (PP) is a well recognized although rare and peculiar complication of thyrotoxicosis, especially among Chinese Japanese patients. The susceptibility to autoimmune thyroid disease has recently been reported be strongly linked certain immunogenetic factors, increased frequency HLA antigens found in patients with Graves' disease. This study was, therefore, undertaken determine haplotypes men thyrotoxic periodic (TPP). typing 35 TPP 263 normal women demonstrated highly...
Younger gerbils have been found to be more resistant than adults cerebral infarction after carotid ligation. In this study, the perfused area bilateral common occlusion was evaluated in infant, young, and adult Mongolian by carbon black perfusion method assess existence significance of collateral blood vessels between vertebrobasilar circulations. Nineteen were divided into three groups (i.e., young aged 3-4, 5-7, 10-17 weeks, respectively). After artery occlusion, injected directly left...
Abstract We have recently developed a new target plate (BLOTCHIP ® ) for MALDI‐MS. An advantage of this procedure is that it does not require the lowering protein concentrations in test samples prior to analysis. Accordingly, technology enables detection peptides present blood samples, including those would otherwise be adsorbed abundant proteins and thus escape detection. Using technology, we analyzed peripheral patients with pregnancy‐induced hypertension (PIH; most common serious...
Human transmembrane tumor necrosis factor (pro‐TNF) was examined for protein acylation. The cDNA encoding pro‐TNF expressed in both COS‐1 cells and Sf9 metabolic labeling with [ 3 H]myristic or H]palmitic acid attempted. 17 kDa mature TNF secreted from the transfected not labeled, whereas 26 specifically labeled acid. of eliminated by treatment hydroxylamine, indicating that due to palmitoylation a cysteine residue via thioester bond. Site‐directed mutagenesis two residues residing leader...
To report a semiautomated retinal vessel caliber measurement system that measures central artery (vein) equivalent (CRAE [CRVE]) with individual correction for magnification errors under conditions assuming optimal focus.The focusing condition of the subject eye fundus camera optical was individually determined by constructing an model each applying its refractive error, corneal curvature, and axial length (AL) to Gullstrand's schematic eye, adjusting position camera's lenses using eye's...
The in vitro micronucleus (MN) test is an important component of a genotoxicity battery that evaluates chemicals. Although the standard method manually scoring micronucleated (MNed) cells by microscope reliable and method, it laborious time-consuming. A high-throughput assay system for detecting MN automatically has long been desired fields pharmaceutical development or environmental risk monitoring. per se cannot clarify whether mode induction aneugenic clastogenic, this clarification may...
Farnesylation of the activated <i>ras</i> oncogene product by protein farnesyltransferase (FTase) is a critical step for its oncogenic function. Because squalene synthase and FTase recruit farnesyl pyrophosphate as common substrate, we modified (SS) inhibitors to develop inhibitors. Among compounds tested, novel inhibitor termed J-104,871 inhibited rat brain with an IC<sub>50</sub> 3.9 nm in presence 0.6 μm (FPP), whereas it scarcely geranylgeranyltransferase-I or SS. The <i>in vitro</i>...
Background/Purpose Lysine-specific gingipain (Kgp) is a virulence factor secreted from Porphyromonas gingivalis (P. gingivalis), major etiological bacterium of periodontal disease. Keratin intermediate filaments maintain the structural integrity gingival epithelial cells, but are targeted by Kgp to produce novel cytokeratin 6 fragment (K6F). We investigated release K6F and its induction cytokine secretion. Methods present in crevicular fluid disease patients gingipain-treated rat cell...
A patient with IgA-λ-type multiple myeloma who appeared to be secreting salivary-type amylase ectopically is reported. Both the patient's serum and urine contained high levels of amylase. Amylase activity in supernatant cultured cells, obtained from pleural effusion while he had malignant pleuritis, increased almost linearly time cell seeding. The presence IgA S-type cells was demonstrated cytochemically by immunoelectron microscopy. These observations showed that produced these human cells.
(1987). Analysis of Cyclopiazonic Acid by Normal Phase High-performance Liquid Chromatography. Agricultural and Biological Chemistry: Vol. 51, No. 9, pp. 2581-2582.