A5012773452- Genetics, Aging, and Longevity in Model Organisms
- Adipose Tissue and Metabolism
- Fungal and yeast genetics research
- Neuroinflammation and Neurodegeneration Mechanisms
- Circadian rhythm and melatonin
- Genetic Mapping and Diversity in Plants and Animals
- Genomics and Chromatin Dynamics
- Mitochondrial Function and Pathology
- Animal Nutrition and Physiology
- Genetic and phenotypic traits in livestock
- RNA Research and Splicing
- Meat and Animal Product Quality
- Molecular Biology Techniques and Applications
- Gene expression and cancer classification
- CRISPR and Genetic Engineering
- Telomeres, Telomerase, and Senescence
- Biochemical Analysis and Sensing Techniques
- Cancer-related molecular mechanisms research
- Tryptophan and brain disorders
- Genomics, phytochemicals, and oxidative stress
- Growth Hormone and Insulin-like Growth Factors
- Muscle Physiology and Disorders
- RNA and protein synthesis mechanisms
- Biochemical Acid Research Studies
- Birth, Development, and Health
Korea University
2015-2024
National Institute of Animal Science
2009
Chungbuk National University
2009
Kyung Hee University
2008
University of North Carolina at Chapel Hill
2004-2006
Yale University
2005
University of Wisconsin–Madison
1999-2004
University of Alabama
2002
The gene expression profile of the aging process was analyzed in skeletal muscle mice. Use high-density oligonucleotide arrays representing 6347 genes revealed that resulted a differential pattern indicative marked stress response and lower metabolic biosynthetic genes. Most alterations were either completely or partially prevented by caloric restriction, only intervention known to retard mammals. Transcriptional patterns calorie-restricted animals suggest restriction retards causing shift...
High-density DNA microarray measures the activities of several thousand genes simultaneously and gene expression profiles have been used for cancer classification recently. This new approach promises to give better therapeutic measurements patients by diagnosing types with improved accuracy. The Support Vector Machine (SVM) is one methods successfully applied diagnosis problems. However, its optimal extension more than two classes was not obvious, which might impose limitations in...
To provide a global analysis of gene expression in the aging heart, we monitored 9,977 genes simultaneously 5- and 30-month-old male B6C3F 1 mice by using high-density oligonucleotide microarrays several statistical techniques. Aging was associated with transcriptional alterations consistent metabolic shift from fatty acid to carbohydrate metabolism, increased extracellular matrix genes, reduced protein synthesis. Caloric restriction (CR) started at 14 months age resulted 19% inhibition...
Sequence motifs that are potentially recognized by DNA-binding proteins occur far more often in genomic DNA than do observed vivo protein–DNA interactions. To determine how chromatin influences the utilization of particular sites, we compared genome-wide binding location yeast transcription factor Leu3 to on same absence any protein cofactors. We found DNA-sequence motif vitro and was functionally indistinguishable, but bound different locations under two conditions. Accounting for...
The packaging of DNA into nucleosomes influences the accessibility underlying regulatory information. Nucleosome occupancy and positioning are best characterized in budding yeast Saccharomyces cerevisiae, albeit asynchronous cell populations or on individual promoters such as PHO5 GAL1-10. Using FAIRE (formaldehyde-assisted isolation elements) whole-genome microarrays, we examined changes nucleosome throughout mitotic cycle synchronized S. cerevisiae. Perhaps surprisingly, did not exhibit...
Abstract Senescent cells exhibit a reduced response to intrinsic and extrinsic stimuli. This diminished reaction may be explained by the disrupted transmission of nuclear signals. However, this hypothesis requires more evidence before it can accepted as mechanism cellular senescence. A proteomic analysis cytoplasmic fractions obtained from young senescent revealed disruption nucleocytoplasmic trafficking (NCT) an essential feature replicative senescence (RS) at global level. Blocking NCT...
Abstract T cells are vulnerable to age-associated changes. Vitamin E has been shown improve cell functions in the old. We studied gene expression profiles of better understand underlying mechanisms age and vitamin E-induced changes function. Young old C57BL mice were fed diets containing 30 (control) or 500 (supplemented) ppm for 4 wks. Gene assessed using microarray analysis with/without anti-CD3/anti-CD28 stimulation. Genes associated with cytokines/chemokines, transcriptional regulation,...
Mitochondria play a crucial role in eukaryotic cells; the mitochondrial electron transport chain (ETC) generates adenosine triphosphate (ATP), which serves as an energy source for numerous critical cellular activities. However, ETC also deleterious reactive oxygen species (ROS) natural byproduct of oxidative phosphorylation. ROS are considered major cause aging because they damage proteins, lipids, and DNA by oxidation. We analyzed chronological life span, growth phenotype, membrane...
To investigate the effects of electro-acupuncture (EA) treatment on regions remote from application, we measured cellular, enzymatic, and transcriptional activities in various internal tissues healthy rats. The EA was applied to well-identified acupoint ST36 leg. After activity natural killer cells spleen, gene expression hypothalamus, antioxidative enzymes liver red blood cells. increased cell spleen by approximately 44%. It also induced genes related pain, including 5-Hydroxytryptamine...
Rapamycin (RM), a drug that inhibits the mechanistic target of rapamycin (mTOR) pathway and responds to nutrient availability, seemingly mimics effects caloric restriction (CR) on healthy life span. However, extent overlap between RM CR remains incompletely understood. Here, we compared impact cellular metabolic status. Both regimens maintained intracellular ATP through chronological aging process showed enhanced mitochondrial capacity. Comparative transcriptome analysis had stronger global...
To characterize gene expression that is dependent on the strength of calorie restriction (CR), we obtained transcriptome at different levels glucose, which a major energy and carbon source for budding yeast. faithfully mimic mammalian CR in yeast culture, reconstituted grew seeding cells fresh 2% YPD media before inoculating into 2%, 1%, 0.5% 0.25% to reflect strengths. We collected characterized 160 genes responded based rigorous statistical analyses multiple test corrected ANOVA (adjusted...
In seeking aryl acylamidase (EC 3.5.1.13) acting on an amide bond in p-acetaminophenol (Tylenol™), we identified a novel gene encoding 496 residues of protein. The revealed conserved amidase signature region with canonical catalytic triad. was expressed E. coli and characterized for its biochemical properties. optimum pH temperature the activity were 10 37°C, respectively. half-life enzyme at 37°C 192 h 90% remained after 3 incubation 40°C. Divalent metals found to inhibit enzyme. K m values...