A5020372110- Telomeres, Telomerase, and Senescence
- Education, Safety, and Science Studies
- DNA Repair Mechanisms
- Real-time simulation and control systems
- Mitochondrial Function and Pathology
- Genomics and Chromatin Dynamics
- Nuclear Structure and Function
- Genetics, Aging, and Longevity in Model Organisms
- Hydraulic and Pneumatic Systems
- Magnetic Bearings and Levitation Dynamics
- Marine and Coastal Research
- Robotic Path Planning Algorithms
- Adipose Tissue and Metabolism
- Prosthetics and Rehabilitation Robotics
- Advanced Control Systems Optimization
- Angiogenesis and VEGF in Cancer
- Control and Dynamics of Mobile Robots
- Metabolism, Diabetes, and Cancer
- Diverse Approaches in Healthcare and Education Studies
- Retinal Development and Disorders
- Autophagy in Disease and Therapy
- Cancer, Hypoxia, and Metabolism
- Wound Healing and Treatments
- Robotics and Sensor-Based Localization
- Internet of Things and Social Network Interactions
Daegu Gyeongbuk Institute of Science and Technology
2016-2024
Institute of Science and Technology
2024
Johns Hopkins University
2012-2022
Inha University
2006-2017
Samsung (South Korea)
2005-2017
Howard Hughes Medical Institute
2007
Samsung Medical Center
2004-2005
Sungkyunkwan University
2004-2005
Pyruvate kinase isoform M2 (PKM2) plays an important role in the growth and metabolic reprogramming of cancer cells stress conditions. Here, we report that SAICAR (succinylaminoimidazolecarboxamide ribose-5'-phosphate, intermediate de novo purine nucleotide synthesis pathway) specifically stimulates PKM2. Upon glucose starvation, cellular concentration increased oscillatory manner stimulated PKM2 activity cells. Changes amounts altered energy level, uptake, lactate production. The...
Endothelial progenitor cells (EPCs) act as endothelial precursors that promote new blood vessel formation and increase angiogenesis by secreting growth factors cytokines in ischemic tissues. These facts prompt the hypothesis EPC transplantation should accelerate wound-repair process facilitating neovascularization production of various molecules related to wound healing. In a murine dermal excisional model, accelerated re-epithelialization compared with mature (ECs) control mice. When wounds...
Recent developments in genome sequencing have expanded the knowledge of genetic factors associated with late-onset Alzheimer's disease (AD). Among them, variant ε4 APOE gene (APOE4) confers greatest risk. Dysregulated glucose metabolism is an early pathological feature AD. Using isogenic ApoE3 and ApoE4 astrocytes derived from human induced pluripotent stem cells, we find that increases glycolytic activity but impairs mitochondrial respiration astrocytes. Ultrastructural autophagy flux...
Scaffolds for the production of cultivated meat, a promising sustainable meat alternative, should exhibit physical and chemical properties that enable three-dimensional animal cell culture, along with biological characteristics support attachment, proliferation, differentiation. Additionally, scaffold be crafted from edible materials offer textural similarities to have minimal influence on flavor taste. Herein, an alginate-based alginate-cellulose hydrogel (ACe-gel) derived brown alga...
Hutchinson-Gilford progeria syndrome (HGPS) constitutes a genetic disease wherein an aging phenotype manifests in childhood. Recent studies indicate that reactive oxygen species (ROS) play important roles HGPS progression. Thus, pharmacological reduction ROS levels has been proposed as potentially effective treatment for patient with this disorder. In study, we performed high-throughput screening to find compounds could reduce fibroblasts and identified rho-associated protein kinase (ROCK)...
Abstract Senescent cells exhibit a reduced response to intrinsic and extrinsic stimuli. This diminished reaction may be explained by the disrupted transmission of nuclear signals. However, this hypothesis requires more evidence before it can accepted as mechanism cellular senescence. A proteomic analysis cytoplasmic fractions obtained from young senescent revealed disruption nucleocytoplasmic trafficking (NCT) an essential feature replicative senescence (RS) at global level. Blocking NCT...
Abstract Selective removal of senescent cells, or senolytic therapy, has been proposed to be a potent strategy for overcoming age-related diseases and even reversing aging. We found that nintedanib, tyrosine kinase inhibitor, selectively induced the death primary human dermal fibroblasts undergoing RS. Similar ABT263, well-known agent, nintedanib triggered intrinsic apoptosis in cells. Additionally, at concentration producing effect, arrested cell cycle nonsenescent cells G1 phase without...
Abstract Background Muscle wasting, resulting from aging or pathological conditions, leads to reduced quality of life, increased morbidity, and mortality. Much research effort has been focused on the development exercise mimetics prevent muscle atrophy weakness. In this study, we identified indoprofen a screen for peroxisome proliferator‐activated receptor γ coactivator α (PGC‐1α) inducers report its potential as drug wasting. Methods The effects treatment dexamethasone‐induced in mice...
The maintenance of mitochondrial function is closely linked to the control senescence. In our previous study, we uncovered a novel mechanism in which senescence amelioration normal aging cells mediated by recovered upon
Human pyruvate kinase isoform M2 (PKM2) is a glycolytic enzyme implicated in cancer. Malignant cancer cells have higher levels of dimeric PKM2, which regarded as an inactive form tetrameric kinase. This perceived inactivity has fueled controversy about how the might contribute to Here we investigate enzymatic properties PKM2(G415R), variant derived from patient, show by size-exclusion chromatography and small-angle X-ray scattering be dimer that cannot tetramer solution. Although PKM2(G415R)...
The selective removal of senescent cells by senolytics is suggested as a potential approach to reverse aging and extend lifespan.Using high-throughput screening with replicative senescence human diploid fibroblasts (HDFs), we identified novel senolytic drug R406 that showed toxicity in cells.Using flow cytometry caspase expression analysis, confirmed caused apoptotic cell death along morphological changes cells.Interestingly, altered the survival-related molecular processes including...
Abstract Background Perturbation in cell adhesion and growth factor signalling satellite cells results decreased muscle regenerative capacity. Cdon (also called Cdo) is a component of complexes implicated myogenic differentiation, but its role regeneration remains to be determined. Methods We generated inducible cell‐specific ablation mice by utilizing conditional allele Pax7 CreERT2 . To induce ablation, were intraperitoneally injected with tamoxifen (tmx). Using cardiotoxin‐induced injury,...
An effective healing response is critical to healthy aging. In particular, energy homeostasis has become increasingly recognized as a factor in skin regeneration. ANT2 mediator of adenosine triphosphate import into mitochondria for homeostasis. Although and mitochondrial integrity are wound healing, the role played by repair process had not been elucidated date. our study, we found that expression decreased aged cellular senescence. Interestingly, overexpression mouse accelerated...
The biological process of aging is thought to result in part from accumulation senescent cells organs. However, the present study identified a subset fibroblasts and smooth muscle which are major constituents organ stroma neither proliferative nor tissues elderly, we termed "mid-old status" cells. Upregulation pro-inflammatory genes (IL1B SAA1) downregulation anti-inflammatory (SLIT2 CXCL12) were detected mid-old In stroma, SAA1 promotes development inflammatory microenvironment via...
Pulmonary hypertension (PH) is characterized by structural and functional changes in the lung including proliferation of vascular smooth muscle cells (VSMCs) excessive collagen synthesis. Although connective tissue growth factor (CTGF) known to promote cell proliferation, migration, adhesion, extracellular matrix production various tissues, studies on role CTGF pulmonary have been limited. Here, we examined expression tissues male Sprague Dawley rats treated with monocrotaline (MCT, 60...