A5004876274- 3D Printing in Biomedical Research
- Microfluidic and Bio-sensing Technologies
- Cancer Cells and Metastasis
- Microfluidic and Capillary Electrophoresis Applications
- Cancer Genomics and Diagnostics
- Biosensors and Analytical Detection
- Hereditary Neurological Disorders
- Muscle Physiology and Disorders
- Neuroscience and Neural Engineering
- Innovative Microfluidic and Catalytic Techniques Innovation
- Genomics and Phylogenetic Studies
- Advanced Biosensing Techniques and Applications
- Single-cell and spatial transcriptomics
- Advanced biosensing and bioanalysis techniques
- Nanofabrication and Lithography Techniques
- Nerve injury and regeneration
- Immune Cell Function and Interaction
- Adipose Tissue and Metabolism
- Pluripotent Stem Cells Research
- CAR-T cell therapy research
- Plant Pathogens and Fungal Diseases
- Botulinum Toxin and Related Neurological Disorders
- Advanced Proteomics Techniques and Applications
- Nanopore and Nanochannel Transport Studies
- Gene expression and cancer classification
Daegu Gyeongbuk Institute of Science and Technology
2017-2025
NovaCell Technology (South Korea)
2022-2024
Konyang University
2015-2017
Samsung (South Korea)
2010-2015
Yong In University
2015
Government of the Republic of Korea
2015
Korea Advanced Institute of Science and Technology
2006-2012
Isolation of circulating tumor cells (CTCs) by size exclusion can yield poor purity and low recovery rates, due to large variations in CTCs, which may overlap with leukocytes render size-based filtration methods unreliable. This report presents a very sensitive, selective, fast, novel method for isolation detection CTCs. Our assay platform consists three steps: (i) capturing CTCs anti-EpCAM conjugated microbeads, (ii) removal unwanted hematologic (e.g., leukocytes, erythrocytes, etc.)...
Circulating tumor cells (CTCs) have gained increasing attention as physicians and scientists learn more about the role these extraordinarily rare play in metastatic cancer. In developing CTC technology, critical criteria are high recovery rates purity. Current isolation methods suffer from an inherent trade-off between two goals. Moreover, ensuring minimal cell stress robust reproducibility is also important for clinical application of CTCs. this paper, we introduce a novel technology using...
Analysis of cancer cells: A new technique isolates rare circulating tumor cells (CTCs) and analyzes their protein expression by the use detachable beads high-pore-density filters. This shows significantly improved efficiency in isolation CTCs from peripheral blood enables accurate measurement situ protein-expression levels.
Background Biomarkers play a key role in risk assessment, assessing treatment response, and detecting recurrence the investigation of multiple biomarkers may also prove useful accurate prediction prognosis cancers. Immunohistochemistry (IHC) has been major diagnostic tool to identify therapeutic subclassify breast cancer patients. However, there is no suitable IHC platform for multiplex assay toward personalized therapy. Here, we report microfluidics-based multiplexed (MMIHC) that...
Abstract This work demonstrates a novel microfluidic in vitro cultivation system for embryos that improves their development using partially constricted channel mimics peristaltic muscle contraction. Conventional photolithography and PDMS replica molding process were used to make straight or microchannels. To investigate the effects of constriction geometry on embryonic development, different widths designed. Bovine loaded incubated by simply placing them tilting machine provide embryo...
Full automation with high purity for circulating tumor cell (CTC) isolation has been regarded as a key goal to make CTC analysis "bench-to-bedside" technology. Here, we have developed novel centrifugal microfluidic platform that can isolate the rare cells from large volume of whole blood. To CTCs blood, introduce disc device having biggest sample capacity well manipulating blood first time. The fully automated could handle 5 mL by designing chamber triangular obstacle structure (TOS) lateral...
Understanding cancer heterogeneity is essential to finding diverse genetic mutations in metastatic cancers.Thus, it critical isolate all types of CTCs identify accurate information from patients.Moreover, full automation robustly capturing the spectrum an urgent need for CTC diagnosis be routine clinical practice.Methods: Here we report capture heterogeneous populations using fully automated, negative depletion-based continuous centrifugal microfluidics (CCM).Results: The CCM system...
Abstract Background Muscle wasting, resulting from aging or pathological conditions, leads to reduced quality of life, increased morbidity, and mortality. Much research effort has been focused on the development exercise mimetics prevent muscle atrophy weakness. In this study, we identified indoprofen a screen for peroxisome proliferator‐activated receptor γ coactivator α (PGC‐1α) inducers report its potential as drug wasting. Methods The effects treatment dexamethasone‐induced in mice...
Peroxisome proliferator–activated receptor-γ coactivator-1α (PGC-1α) is a master regulator of mitochondrial biogenesis. Reduced PGC-1α abundance linked to skeletal muscle weakness in aging or pathological conditions, such as neurodegenerative diseases and diabetes; thus, elevating might be promising strategy treat aging. Here, we performed high-throughput screening identified natural compound, farnesol, potent inducer PGC-1α. Farnesol administration enhanced oxidative capacity strength,...
Abstract Human colorectal cancer cell lines (HT29 and HCT116) were exposed to dielectric barrier discharge (DBD) plasma at atmospheric pressure investigate the anticancer capacity of plasma. The dose- time-dependent effects DBDP on viability, regulation transcription factor Sp1, cell-cycle analysis, colony formation investigated by means MTS assay, DAPI staining, propidium iodide annexin V–FITC Western blot RT-PCR fluorescence microscopy, anchorage-independent transformation assay. By...
Nutritional status is a determining factor for growth during development and homeostatic maintenance in adulthood. In the context of muscle, hormone (GH) coordinates with nutritional status; however, detailed mechanisms remain to be fully elucidated. Here, we show that transcriptional repressor B cell lymphoma 6 (BCL6) maintains muscle mass by sustaining GH action. Muscle-specific genetic deletion BCL6 at either perinatal or adult stages profoundly reduces compromises strength. Conversely,...
Kahweol, a diterpene molecule, has antiproliferative effects on several types of human cancer cells, but whether it apoptotic effect in non-small cell lung (NSCLC) is not known. To explore this possibility, we incubated cells from two NSCLC lines, NCI-H358 and NCI‑H1299, with different concentrations kahweol used the MTS assay, DAPI staining, propidium iodide Annexin V immunocytochemical test, western blot analysis to characterize molecule signaling pathway underlying its effects. The...
In spite of recent developments in mass spectrometry imaging techniques, high-resolution multiplex protein bioimaging techniques are required to unveil the complex inter- and intracellular biomolecular interactions for accurate understanding life phenomena disease mechanisms. Herein, we report with secondary ion (SIMS) using metal oxide nanoparticle (MONP)-conjugated antibodies <300 nm spatial resolution low dose without beam damage because high yields MONPs, which can provide simultaneous...
A Gram-reaction-positive, non-motile, non-spore-forming, aerobic rod, designated BXN5-15(T), was isolated from the soil of a ginseng field on Baekdu Mountain in China. Strain BXN5-15(T) grew optimally at 30 °C and pH 6.0-7.0 absence NaCl R2A agar. displayed β-glucosidase activity, which allowed it to transform ginsenoside Rb(1) (one dominant active components ginseng) via Rd minor F(2). On basis 16S rRNA gene sequence analysis, strain shown belong genus Solirubrobacter. The closest...
This communication presents a microfluidic method for size-based cell sorting, which provides simple and robust approach cycle synchronization by manual stand-alone operation.
Abstract Circulating tumor cells (CTCs), though exceedingly rare in the blood, are nonetheless becoming increasingly important cancer diagnostics. Despite this keen interest and growing number of potential clinical applications, there has been limited success developing a CTC isolation platform that simultaneously optimizes recovery rates, purity, cell compatibility. Herein, novel tracheal carina‐inspired bifurcated (TRAB) microfilter system is reported, which uses an optimal filter gap size...
Smart microfluidic pipette tip: A method for removing cells and recovering liquid medium is presented applied blood cell rejection cytotoxicity assay. This enables continuous by manual operation, potentially providing the means rapid, inexpensive sample preparation personalized diagnostics mobile laboratory.
A Gram-stain-negative, rod-shaped, non-spore-forming, oxidase and catalase-positive, strictly aerobic bacterium, designated strain Gsoil 524T, was isolated from the soil of a ginseng field subjected to polyphasic taxonomic analysis. Phylogenetic analysis, based on 16S rRNA gene sequence, placed 524T in distinct lineage family Sphingobacteriaceae, sharing 87.2-88.0 % sequence similarity with members closely related genera Pedobacter, Mucilaginibacter Solitalea. Strain contained MK-7 as...
As paper-based diagnostics has become predominantly driven by more advanced microfluidic technology, many of the research efforts are still focused on developing reliable and versatile fluidic control devices, apart from improving sensitivity reproducibility. In this work, we introduce a novel robust paper system enabling control. The comprises linear push-pull solenoid an Arduino Uno microcontroller. precisely controlled pressure exerted stops flow. We first determined stroke distance to...
A Gram-stain-negative, strictly aerobic, rod-shaped bacterium, designated W1-2-1T, was isolated from tap water in South Korea. The strain characterized by a polyphasic approach to clarify its taxonomic position. Strain W1-2-1T grew at 18-42 °C and pH 6.0-10.0 on R2A medium. Phylogenetic analysis based 16S rRNA gene sequences indicated that the isolate belongs genus Sphingomonas is most closely related oligophenolica JCM 12082T (97.2 % similarity), asaccharolyticaNBRC 15499T (96.8 %),...