A5001709379- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Cancer-related gene regulation
- Cancer-related Molecular Pathways
- Microtubule and mitosis dynamics
- Genetic factors in colorectal cancer
- Cellular Mechanics and Interactions
- Lung Cancer Treatments and Mutations
- Hippo pathway signaling and YAP/TAZ
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Esophageal Cancer Research and Treatment
- Protein Kinase Regulation and GTPase Signaling
- Ferroptosis and cancer prognosis
- Genomic variations and chromosomal abnormalities
- Helicobacter pylori-related gastroenterology studies
- Signaling Pathways in Disease
- Telomeres, Telomerase, and Senescence
- Genetics and Neurodevelopmental Disorders
- Lung Cancer Research Studies
- Nerve injury and regeneration
- Wnt/β-catenin signaling in development and cancer
- Melanoma and MAPK Pathways
- Pancreatic function and diabetes
- RNA and protein synthesis mechanisms
Sungkyunkwan University
2008-2023
Samsung (South Korea)
2007-2016
Samsung Medical Center
2004-2013
Pusan National University
2010
Biomedical Research Institute
2004-2009
Samsung (United Kingdom)
2006
Abstract Purpose: To identify epigenetic molecular makers in plasma for the early detection of colorectal cancer. Experimental Design: We retrospectively analyzed methylation status 10 genes fresh-frozen tissues and corresponding samples from 243 patients with stage I II sporadic cancer, 276 healthy individuals, 64 adenoma using methylation-specific PCR. The score (Mscore) was used to find markers high sensitivity specificity. Results: Of cancer tissues, detected 18% p14, 34% p16, 27% APC,...
Purpose The aim of this study was to identify tumor-specific methylation in bronchial lavage for the early detection non-small-cell lung cancer (NSCLC) by differentiating age-related from NSCLC. Patients and Methods Eighty-five NSCLC patients 127 cancer-free subjects participated study. Aberrant at promoters p16, Ras association domain family 1A (RASSF1A), fragile histidine triad (FHIT), H-cadherin, retinoic acid receptor β (RARβ) genes were evaluated resected tumor tissues samples...
Abstract BACKGROUND. Despite many reports about the involvement of DNA methyltransferases (DNMTs) in human cancers, including nonsmall cell lung cancer (NSCLC), clinicopathologic significance DNMTs primary NSCLC remains to be elucidated. METHODS. The relation between mRNA levels (1 and 3b) promoter methylation p16, RARβ2, H‐cadherin, GSTP1, RIZ, FHIT genes features 102 fresh‐frozen tissues paraffin blocks were retrospectively studied. assessed via semiquantitative reverse‐transcription...
Abstract Despite advances in the detection and treatment of lung cancer, prognosis for patients with cancer is poor, partly as a result recurrences. We retrospectively analyzed relationship between recurrence survival non–small cell cancers (NSCLC), promoter methylation p16, GSTP1, FHIT, H-cadherin, RARβ2 genes to identify prognostic molecular marker associated NSCLC. Methylation status from 335 paraffin blocks was determined by methylation-specific PCR. Of NSCLC samples, detected 35% 39%...
Peroxisome proliferator–activated receptor-γ coactivator-1α (PGC-1α) is a master regulator of mitochondrial biogenesis. Reduced PGC-1α abundance linked to skeletal muscle weakness in aging or pathological conditions, such as neurodegenerative diseases and diabetes; thus, elevating might be promising strategy treat aging. Here, we performed high-throughput screening identified natural compound, farnesol, potent inducer PGC-1α. Farnesol administration enhanced oxidative capacity strength,...
The prognosis of esophageal squamous cell carcinoma (ESCC) patients remains very poor, which is partially due to a high rate recurrence. This study was aimed at identifying recurrence-associated epigenetic prognostic marker in with ESCC. We retrospectively analyzed the CpG island hypermethylation p16, Wif-1, sFRP1, integrin alpha4, CDH1, DAP kinase and RARbeta2 genes 251 ESCCs. methylation status determined by methylation-specific PCR. Hypermethylation detected 52% for 25% RARbeta2, 43% 21%...
// Dong-Hao Jin 1 , Jeeyun Lee 2 Kyoung Mee Kim 3 Sung 4 Duk-Hwan and Joobae Park Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School Medicine, Suwon, Korea Internal Medical Center, Seoul, Pathology Translational Genomics, Surgery, Correspondence to: Kim, email: Park, Keywords : gastric cancer; copy number alteration; MAPK15; c-Jun; stability Received December 01, 2014 Accepted May 02, 2015 Published 19, Abstract This study was aimed at...
Abstract This study was aimed at investigating the involvement of SUV39H1 histone methyltransferase on epigenetic change euchromatic promoter in colorectal cancer. We retrospectively analyzed mRNA levels and methylation p14 ARF , p16 INK4a HLTF genes as well DNA 1 (DNMT1) fresh frozen tissues from 219 cancer patients. The DNMT1 were assessed via quantitative real‐time PCR profiles CpG islands determined using methylation‐specific PCR. elevated 25% 42% cancers, respectively. hypermethylation...
When we were studying phosphorylated proteins in the rat brain after electroconvulsive shock (ECS), observed rapid phosphorylation of a 75-kDa protein, which cross-reacted with anti-phospho-p70 S6 kinase antibody. The protein was purified and identified as moesin, member ezrin/radixin/moesin (ERM) family general cross-linker between cortical actin filaments plasma membranes. moesin from at serine threonine residues. Moesin rapidly 558 residue ECS hippocampus, peaked 1 min, returned to basal...
Cytoskeleton-associated protein 2 (CKAP2), also known as tumor-associated microtubule-associated (TMAP), is a novel that frequently upregulated in various malignances. However, its cellular functions remain unknown. A previous study has shown level begins to increase during G(1)/S and peaks at G(2)/M, after which it decreases abruptly. Ectopic overexpression of TMAP/CKAP2 induced microtubule bundling related increased stability. resulted cell cycle arrest mitosis due defect centrosome...
Abstract BACKGROUND: The objective of this was to identify functional single nucleotide polymorphisms (SNPs) in cyclin‐dependent kinases (CDKs) and cyclins that are associated with risk human cancer. METHODS: First, 45 SNPs CDKs were analyzed 106 lung cancers 108 controls for a pilot study. One SNP (reference [rs] 769236, +1 guanine adenine [G→A]) at the promoter region cyclin A2 ( CCNA2 ) also 1989 (300 breast cancers, 450 colorectal gastric 367 hepatocellular carcinomas, 422 cancers) 1096...
Background The level of proliferation activity is a strong prognostic or predictive indicator in breast cancer, but its optimal measurement still debate, necessitating new markers. In the present study, significance CKAP2-positive cell count (CPCC), marker, was evaluated, and results were compared with those for mitotic index (MAI). Methods This study included 375 early-stage cancer samples collected from two institutions between 2000 2006. Immunohistochemical staining performed using CKAP2...
Purpose To investigate whether the promoter hypermethylation of retinoic acid receptor beta 2 (RARβ2) is associated with development second primary lung cancers (SPLCs) differentially according to smoking status in non–small-cell cancer (NSCLC). Patients and Methods We retrospectively analyzed relationship between RARβ2 methylation SPLC a total 342 NSCLCs. The was determined by using methylation-specific polymerase chain reaction. difference time log-rank test Cox proportional hazards model....
Long-lived people may have a unique genetic makeup that makes them more resistant than the general population to prevalent age-related diseases; however, not much is known about genes involved in longevity. To identify susceptibility variants controlling longevity, we performed high-throughput candidate gene study using 137 Koreans over 90 yr old and 213 young healthy Koreans. We evaluated 463 informative markers located 176 mostly for diabetes mellitus, cardiovascular disease cancer under...
Abstract This study was aimed at understanding the effects of histone modifications on recurrence-free survival (RFS) after esophagectomy in esophageal squamous cell carcinoma (ESCC). The acetylation H3 lysine (H3K9Ac), 18 (H3K18Ac), and H4 12 (H4K12Ac), dimethylation 9 (H3K9diMe) arginine 3 (H4R3diMe) were analyzed by immunohistochemistry 237 ESCCs. K-means clustering algorithm used to identify unique patterns modifications. At a median follow-up 5.1 years, 109 (46%) patients had developed...
Abstract Purpose: This study was aimed at analyzing the recurrence-related prognostic significance of 12 candidate molecular biomarkers in node-negative stage I–II non–small cell lung cancer (NSCLC). Experimental Design: We retrospectively analyzed promoter methylation eight genes using methylation-specific PCR formalin-fixed and paraffin-embedded tissues from 328 NSCLCs. The expression Bcl-2, E-cadherin, p53, p63 proteins also assessed by immunohistochemistry. Results: Recurrence found 145...
Abstract Despite significant advances in the detection and treatment of hepatocellular carcinoma, prognosis patients with carcinoma remains very poor, part due to high incidence recurrence. This study was aimed at identifying a prognostic indicator recurrence carcinoma. We retrospectively analyzed CpG island hypermethylation p14, p15, p16, GSTP1, integrin α4, SYK, CDH1 genes fresh-frozen tissues from 265 using methylation-specific PCR. The expression levels p16 p53 were evaluated by...
Gastric carcinoma is considered to be one of the most prevalent cancers worldwide. We have performed differential-display polymerase chain reaction (DD-PCR) in order compare gene expression profile gastric and that a normal stomach, an attempt identifiy differentially expressed genes associated with primary human cancers. One down-regulated was identified as regenerating islet-derived 3 α (REG3A), also known hepatocarcinoma-intestine-pancreas/ pancreatitis-associated protein (HIP/PAP). REG3A...