A5034908591- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Osteoarthritis Treatment and Mechanisms
- Respiratory viral infections research
- Viral Infections and Vectors
- HIV/AIDS Research and Interventions
- Bacteriophages and microbial interactions
- HIV Research and Treatment
- Mosquito-borne diseases and control
- CRISPR and Genetic Engineering
- Plant Virus Research Studies
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Tracheal and airway disorders
- COVID-19 and healthcare impacts
- Esophageal and GI Pathology
- Cervical Cancer and HPV Research
- Immune responses and vaccinations
- Viral-associated cancers and disorders
- Neutropenia and Cancer Infections
- Musculoskeletal pain and rehabilitation
- Hematological disorders and diagnostics
- Computational Drug Discovery Methods
- Parvovirus B19 Infection Studies
- Airway Management and Intubation Techniques
- Diverse Scientific Research Studies
Donald & Barbara Zucker School of Medicine at Hofstra/Northwell
2024
University of North Carolina at Chapel Hill
2017-2024
UNC Lineberger Comprehensive Cancer Center
2021-2024
Northwell Health
2024
Segeberger Kliniken
2024
The University of Adelaide
2021
Queen Elizabeth Hospital
2021
Coronaviruses (CoVs) traffic frequently between species resulting in novel disease outbreaks, most recently exemplified by the newly emerged SARS-CoV-2, causative agent of COVID-19. Here, we show that ribonucleoside analog β-d-N
Abstract Mutagenic ribonucleosides can act as broad-based antiviral agents. They are metabolized to the active ribonucleoside triphosphate form and concentrate in genomes of RNA viruses during viral replication. β-d-N4-hydroxycytidine (NHC, initial metabolite molnupiravir) is >100-fold more than ribavirin or favipiravir against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), with activity correlated level mutagenesis virion RNA. However, NHC also displays host mutational...
Abstract Coronaviruses (CoVs) traffic frequently between species resulting in novel disease outbreaks, most recently exemplified by the newly emerged SARS-CoV-2. Herein, we show that ribonucleoside analog β-D-N 4 -hydroxycytidine (NHC, EIDD-1931) has broad spectrum antiviral activity against SARS-CoV 2, MERS-CoV, SARS-CoV, and related zoonotic group 2b or 2c Bat-CoVs, as well increased potency a coronavirus bearing resistance mutations to another nucleoside inhibitor. In mice infected with...
Next generation sequencing (NGS)/deep has become an important tool in the study of viruses. The use unique molecular identifiers (UMI) can overcome limitations PCR errors and PCR-mediated recombination reveal true sampling depth a viral population being sequenced NGS experiment. This approach enhanced sequence data represents ideal to both high low abundance drug resistance mutations more generally explore genetic structure populations. Central UMI/Primer ID is creation template consensus...
The association between low-frequency HIV-1 drug resistance mutations (DRMs) and treatment failure (TF) is controversial. We explore this using NGS methods that accurately sample DRMs.
Next generations sequencing (NGS) has become an important tool in biomedical research. The Primer ID approach combined with the MiSeq platform overcomes limitation of PCR errors and reveals true sampling depth population sequencing, making it ideal to study mutagenic effects potential broad-spectrum antivirals on RNA viruses. In this report we describe a protocol using mutations induced by coronavirus genome from vitro cell culture model vivo mouse model. Viral or total lung tissue is tagged...
A diverse group of RNA viruses have the ability to gain access central nervous system (CNS) and cause severe neurological disease. Current treatment for people with this type infection is generally limited supportive care. To address need reliable antivirals, we utilized a strategy lethal mutagenesis limit virus replication. We evaluated ribavirin (RBV), favipiravir (FAV) N 4 -hydroxycytidine (NHC) against La Crosse (LACV), which one most common causes pediatric arboviral encephalitis cases...
A diverse group of RNA viruses including Rabies, Polio, La Crosse, West Nile, Zika, Nipah, Eastern and Western equine encephalitis, Venezuelan Japanese tick-borne encephalitis have the ability to gain access replicate in central nervous system (CNS), causing severe neurological disease. Current treatment for these patients is generally limited supportive care. To address need a generalizable antiviral, we utilized strategy mutagenesis limit virus replication. We evaluated ribavirin (RBV),...
Background: Elevated levels of systemic inflammation are common in people with osteoarthritis and predict both pain structural outcomes. Krill oil has anti-inflammatory properties reduces severity inflammatory arthritis mice by 50% compared to controls. 1 In humans, krill reduced knee function two short, moderate quality randomised controlled trials (RCTs) osteoarthritis. However, evidence from longer imaging data is lacking. Objectives: The aim this study was compare the efficacy (2g / day)...
Abstract Background The ongoing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic posed an unpreceded threat to the management of other pandemics such as human immunodeficiency virus-1 (HIV-1) in United States. full impact SARS-CoV-2 on HIV-1 needs be evaluated. Methods All individuals with newly reported diagnoses from NC State Laboratory Public Health were enrolled this prospective observational study, 2018–2021. We used a sequencing-based recency assay identify recent...