A5043544940- Bone Metabolism and Diseases
- Bone health and treatments
- Growth Hormone and Insulin-like Growth Factors
- Trace Elements in Health
- Advancements in Battery Materials
- S100 Proteins and Annexins
- Cancer, Hypoxia, and Metabolism
- TGF-β signaling in diseases
- Physics of Superconductivity and Magnetism
- Bone health and osteoporosis research
- Axon Guidance and Neuronal Signaling
- Magnetic properties of thin films
- Estrogen and related hormone effects
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Thyroid Disorders and Treatments
- Cancer-related molecular mechanisms research
- Iron Metabolism and Disorders
- RNA Research and Splicing
- Reproductive Biology and Fertility
- Ubiquitin and proteasome pathways
- Retinoids in leukemia and cellular processes
- Barrier Structure and Function Studies
- Hypothalamic control of reproductive hormones
- Pelvic and Acetabular Injuries
- Dermatological and Skeletal Disorders
VA Loma Linda Healthcare System
2012-2025
Jerry L. Pettis Memorial VA Medical Center
2014-2025
Loma Linda University
2015-2025
Loma Linda University Medical Center
2022
Henan Institute of Science and Technology
2020
Third Affiliated Hospital of Southern Medical University
2018
Immunovaccine (Canada)
2009-2011
Peking University
2010
United States Department of Veterans Affairs
2006
Montreal Clinical Research Institute
2000-2003
Targeted disruption of the receptor for glycoprotein hormone, FSH (FSH-R) causes a gene dose-related endocrine and gametogenic abnormality in female mice. The resulting FSH-R knockout (FORKO) mutants have disordered estrous cycles, ovulatory defects, atrophic uterus. heterozygous animals that initially show reduced fertility undergo early reproductive senescence stop breeding altogether. Lack signaling females severe ovarian underdevelopment producing chronic estrogen deficiency. This was...
Increased intracellular iron spurs mitochondrial biogenesis and respiration to satisfy high-energy demand during osteoclast differentiation bone-resorbing activities. Transferrin receptor 1 (Tfr1) mediates cellular uptake through endocytosis of iron-loaded transferrin, its expression increases differentiation. Nonetheless, the precise functions Tfr1 Tfr1-mediated in biology skeletal homeostasis remain incompletely understood. To investigate role lineage cells vivo vitro, we crossed Tfrc...
Table sugar was pyrolyzed under various conditions. Carbons of demonstrated high capacity for Li intercalation, exceeding that graphite. Experiments were carried out to search optimal pyrolyzing The electrochemical behavior these carbons found depend on the pyrolysis temperature, heating rate, argon gas flow and morphology precursors. Pyrolysis also vacuum, which is equivalent a flow. conditions showed large reversible capacity, ≈650 mAh/g, small irreversible ⩽170 hysteresis between...
Mutations of ephrin B1 in humans result craniofrontonasal syndrome. Because little is known the role and mechanism action bone, we examined function osteoblast-produced vivo identified molecular by which reverse signaling regulates bone formation. Targeted deletion gene type 1alpha2 collagen-producing cells resulted severe calvarial defects, decreased size, mineral density, trabecular volume, caused impairment osterix expression osteoblast differentiation. Coimmunoprecipitation TAZ complex...
Understanding how bone growth is regulated by hormonal and mechanical factors during early periods important for optimizing the attainment of peak mass to prevent or postpone occurrence fragility fractures later in life. Using genetic mouse models that are deficient thyroid hormone (TH) (Tshr(-/-) Duox2(-/-)), (GH) (Ghrhr(lit/lit)), both (Tshr(-/-); Ghrhr(lit/lit)), we demonstrate there an period prior puberty when effects GH surprisingly small TH plays a critical role regulation skeletal...
ABSTRACT Thyroid hormones (THs) are known to regulate endochondral ossification during skeletal development via acting directly in chondrocytes and osteoblasts. In this study, we focused on TH effects the secondary center (SOC) because time of appearance SOCs several species coincides with when peak levels attained. Accordingly, micro–computed tomography (µCT) evaluation femurs tibias at day 21 TH-deficient control mice revealed that is severely compromised owing deficiency treatment for 10...
Abstract To identify the genes and signal pathways responsible for mechanical loading‐induced bone formation, we evaluated differential gene expression on a global basis in tibias of C57BL/6J (B6) mice after four days four‐point bending. We applied loads to right B6 at 9 N, 2 Hz 36 cycles per day, with left used as unloaded controls. RNA from was harvested 24 h last stimulation subjected microarray. Of 20,280 transcripts hybridized array, 346 were differentially expressed loaded bones...
ABSTRACT To assess the roles of Lrrk1 and Lrrk2, we examined skeletal phenotypes in Lrrk2 knockout (KO) mice. KO mice exhibit severe osteopetrosis caused by dysfunction multinucleated osteoclasts, reduced bone resorption endocortical trabecular regions, increased mineralization. have lifelong accumulation respond normally to anabolic actions teriparatide treatment, but are resistant ovariectomy-induced boss. Precursors derived from differentiate into cells response macrophage...
Abstract Mutations in PLEKHM1 cause osteopetrosis humans and rats. The germline osteoclast conditional deletions of Plekhm1 gene mice lead to defective bone resorption increased trabecular mass without overt abnormalities other organs. As an adaptor protein, pleckstrin homology RUN domain containing M1 (PLEKHM1) interacts with the key lysosome regulator small GTPase RAB7 via its C-terminal RUBICON homologous (RH) domain. In this study, we have conducted a structural-functional study RH...
There are three FAM98 family proteins (FAM98A/B/C) in humans and mice. Their physiological functions remain largely unknown. We have previously reported that Fam98a interacts with Plekhm1 murine osteoclasts lysosome trafficking/secretion bone resorption vitro. In this study, we found all Fam98 genes were expressed precursor mature osteoclasts. While the knockdown of Fam98c by a specific short-hairpin RNA (shRNA) osteoclast precursors attenuated osteoclastogenesis, depletion Fam98b an shRNA...
Global knockout (KO) of the Lrrk1 gene in mice causes severe osteopetrosis because failure osteoclasts to resorb bone. The molecular mechanism LRRK1 regulation osteoclast function is not fully understood. Here, we performed a 2D DIGE phosphor-proteomics analysis identify potential targets osteoclasts. Splenocytes from KO and wild-type (WT) were differentiated into for protein extraction. Lysates WT cells labeled with Cy3- Cy5-dye, respectively. Labeled proteins mixed analyzed on same SDS...
Different polysiloxanes were pyrolyzed to make silicon oxycarbide glasses which have been shown be possible candidates for anode materials of Li-ion batteries. The decomposition process during pyrolysis was studied using a thermal gravimetric analyzer connected residual gas three representative polymers. No silicon- or oxygen-containing gases observed pyrolysis. stoichiometry all the final chars measured and found agree well with predictions calculated initial polymer stoichiometry, ceramic...
Previous in vitro studies found that nuclear factor erythroid-derived 2-like 1 (NFE2L1) was involved mediating ascorbic acid-induced osterix expression and osteoblast differentiation via binding to the antioxidant response element of promoter. To test role NFE2L1 regulating bone formation vivo, we disrupted specifically osteoblasts. Mice expressing Cre under control Col1alpha2 promoter were crossed with loxP mice generate Cre+ knockout (KO) Cre- wild-type (WT) mice. Skeletal measurements by...
Mouse genetic studies reveal that ascorbic acid (AA) is essential for osteoblast (OB) differentiation and osterix (Osx) was a key downstream target of AA action in OBs. To determine the molecular pathways regulation Osx expression, we evaluated if regulates expression by regulating production and/or actions local growth factors extracellular matrix (ECM) proteins. Inhibition IGFs inhibitory IGFBP-4, BMPs noggin, ECM-mediated integrin signaling RGD did not block effects on Furthermore,...
1,25-Dihydroxyvitamin D3 (1,25(OH)2D3) suppresses autoimmunity and inflammation; however, the mechanism of its action has not been fully understood. We sought in this study to determine whether anti-immune/anti-inflammatory 1,25(OH)2D3 is part mediated through an interplay between toll-like receptor (TLR)7/8 signaling. treatment prior and/or following experimental autoimmune encephalomyelitis (EAE) induction effectively reduced inflammatory cytokine expression spinal cord ameliorated EAE....
Osteosclerotic metaphyseal dysplasia (OSMD) is a unique form of osteopetrosis characterised by severe osteosclerosis localised to the bone ends. The mode inheritance autosomal recessive. Its genetic basis not known.To identify disease gene for OSMD.By whole exome sequencing in boy with OSMD, we identified homozygous 7 bp deletion (c.5938_5944delGAGTGGT) LRRK1 gene. His skeletal phenotype recapitulated that seen Lrrk1-deficient mouse. shared hallmarks included sclerosis undermodelled...
Surgical treatment of both-column acetabular fractures is challenging because the complex fracture patterns and curved surface acetabulum. Seldom study has compared application three-dimensional (3D) printing technology traditional methods contouring plates intra-operatively for surgical fractures. We presented use both 3D a virtual simulation in pre-operative planning hypothesized that will assist orthopedic surgeons shortening time improving clinical outcomes.Forty patients with were...
Mapping of the magnetic flux distribution in a square-shaped superconducting YBa2Cu3O7 thin film was carried out using scanning micro-Hall probe with spatial resolution about 25 μm. Calculation procedures for converting measured map to current are discussed. The penetration applied field perpendicular surface studied initially zero-field-cooled samples. With full penetration, sheet currents follow sample edges, whereas partial more complex and include multiply connected patterns. In both...